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• W2095413963 abstract "A new general J-HMQC-based technique is presented, which allows an accurate determination of heteronuclear coupling constants. The most important feature of this new approach includes acquisition of the two data sets with and without the additional pi(S)-pulse at the end of coupling evolution period. This enables preservation and separation of the two orthogonal terms of coupling evolution, which are manifested by in- and antiphase cross-peaks, respectively. The coupling magnitudes are evaluated by the nonlinear least-squares fitting of the ratios of integrated signal volumes for both kinds of signals. The effectiveness of the new sequence is demonstrated by determination of the 3J(H3'(i),P(i+1)) couplings in DNA octamer duplex d(GCGTACGC)(2) sample. Additionally, the ability of the new method for the measurement at the natural abundance level of 13C nuclei is presented for the beta-cyclodextrin." @default.
• W2095413963 created "2016-06-24" @default.
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• W2095413963 date "2003-02-01" @default.
• W2095413963 modified "2023-09-25" @default.
• W2095413963 title "The new HMQC-based technique for the quantitative determination of heteronuclear coupling constants. Application for the measurement of in DNA oligomers" @default.
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• W2095413963 doi "https://doi.org/10.1016/s1090-7807(02)00142-8" @default.
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