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• W2095443384 abstract "The occurrence and form of selenium and mercury were investigated in Indian Mustard, Brassica juncea, a selenium accumulating plant, which had been co-exposed to varying concentration levels of these two elements. Plants were grown and exposed in hydroponic solutions. Following exposure, root exudates were collected in fresh solutions and the head-space around the aerial portions of the plants was sampled. These samples and the harvested plant tissues were then processed for determination of Se and Hg-containing compounds. For the plant tissues, roots, stems and leaves were separated and extracted using a sequential procedure that removed water-soluble species, water-soluble proteins, and dodecyl sulfate-soluble proteins. Size exclusion chromatography allowed further fractionation. High molecular-weight selenium/mercury-containing compounds were found primarily in the plant root extract. Evidence suggests that a Se and Hg complex of high molecular weight may be protein associated. For the analysis of exudate solutions, ion-pairing reversed phase chromatography coupled to ICP-MS was used. Multiple selenium and mercury species were detected, with one mercury-containing compound observed eluting near selenocystine. Plant head-space was sampled with solid phase microextraction and analyzed with GC-ICP-MS and GC-TOFMS. Apart from the primary selenium volatiles and elemental mercury, no volatile species simultaneously containing Se and Hg could be detected." @default.
• W2095443384 created "2016-06-24" @default.
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• W2095443384 date "2006-01-01" @default.
• W2095443384 modified "2023-10-04" @default.
• W2095443384 title "Localization and speciation of selenium and mercury in Brassica juncea—implications for Se–Hg antagonism" @default.
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• W2095443384 doi "https://doi.org/10.1039/b514954a" @default.
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