Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095471664> ?p ?o ?g. }
- W2095471664 abstract "Incubation of monolayer cultures of pig hepatocytes with furazolidone resulted in increased intracellular oxidized glutathione (GSSG) levels but no decreased reduced glutathione (GSH) levels. However, a clear decrease in GSH levels was observed at the higher drug concentrations when either the synthesis of GSH was blocked with buthionine-d,l-sulphoximine (BSO) or the reduction of GSSG by 1,3-bis(2-chloroethyl)-1-nitrosourea. Furthermore, when cells containing (35)S-labelled GSH were exposed to furazolidone, a dose-related loss of radiolabelled GSH was observed. The increased loss was balanced by elevated levels in the medium of GSSG and a second compound, probably the disulphide of cysteine and GSH. The biotransformation of (14)C-furazolidone by cells resulted partly in the formation of the cyano metabolite as well as a large number of more hydrophilic unknown metabolites. No evidence was obtained for the excretion by the cells of the glutathione conjugate that was previously detected in microsomal incubations. This could, however, be due to the unstable nature of the conjugate, as demonstrated by its rapid disappearance when added to the medium. In the presence but not the absence of cells, this partly resulted in the formation of the cyano metabolite, which on prolonged incubation was further metabolized into more polar metabolites. Increased LDH leakage at high drug concentrations could be observed only when GSH levels were kept at a low level with BSO. Contrary to previous observations with microsomes, decreased GSH levels did not result in increased formation of protein-bound metabolites of furazolidone and the cyano metabolite. In addition, GSH depletion did not result in increased inhibition of the pyruvate metabolism by furazolidone. No evidence was obtained for the presence of reactive thiol conjugates of furazolidone in the protein fraction of cells incubated with the drug. It is concluded that GSH has an active role in the protection of cells against the cytotoxic effects of furazolidone related to oxidative stress, but that no evidence could be obtained for the formation and excretion of reactive thiol conjugates." @default.
- W2095471664 created "2016-06-24" @default.
- W2095471664 creator A5003469918 @default.
- W2095471664 creator A5027390486 @default.
- W2095471664 creator A5056204176 @default.
- W2095471664 creator A5089727041 @default.
- W2095471664 date "1992-05-01" @default.
- W2095471664 modified "2023-09-27" @default.
- W2095471664 title "Study on the role of glutathione in the biotransformation and toxicity of furazolidone using pig hepatocytes" @default.
- W2095471664 cites W1535367955 @default.
- W2095471664 cites W1535828429 @default.
- W2095471664 cites W1563428629 @default.
- W2095471664 cites W1580968270 @default.
- W2095471664 cites W1583993235 @default.
- W2095471664 cites W1699447737 @default.
- W2095471664 cites W1965596803 @default.
- W2095471664 cites W1970749702 @default.
- W2095471664 cites W1974932331 @default.
- W2095471664 cites W1977344741 @default.
- W2095471664 cites W1983678579 @default.
- W2095471664 cites W1984905958 @default.
- W2095471664 cites W1984942439 @default.
- W2095471664 cites W2005156172 @default.
- W2095471664 cites W2017816384 @default.
- W2095471664 cites W2018831104 @default.
- W2095471664 cites W2019351386 @default.
- W2095471664 cites W2025357993 @default.
- W2095471664 cites W2027376850 @default.
- W2095471664 cites W2063925889 @default.
- W2095471664 cites W2068062638 @default.
- W2095471664 cites W2073400155 @default.
- W2095471664 cites W2081559554 @default.
- W2095471664 cites W2094406725 @default.
- W2095471664 cites W2102131116 @default.
- W2095471664 cites W2118420081 @default.
- W2095471664 cites W2138184713 @default.
- W2095471664 cites W2164276159 @default.
- W2095471664 cites W2343269492 @default.
- W2095471664 cites W2422561637 @default.
- W2095471664 cites W2460875008 @default.
- W2095471664 cites W2489005718 @default.
- W2095471664 cites W250485630 @default.
- W2095471664 doi "https://doi.org/10.1016/0887-2333(92)90036-q" @default.
- W2095471664 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20732118" @default.
- W2095471664 hasPublicationYear "1992" @default.
- W2095471664 type Work @default.
- W2095471664 sameAs 2095471664 @default.
- W2095471664 citedByCount "10" @default.
- W2095471664 countsByYear W20954716642015 @default.
- W2095471664 crossrefType "journal-article" @default.
- W2095471664 hasAuthorship W2095471664A5003469918 @default.
- W2095471664 hasAuthorship W2095471664A5027390486 @default.
- W2095471664 hasAuthorship W2095471664A5056204176 @default.
- W2095471664 hasAuthorship W2095471664A5089727041 @default.
- W2095471664 hasConcept C178790620 @default.
- W2095471664 hasConcept C181199279 @default.
- W2095471664 hasConcept C185592680 @default.
- W2095471664 hasConcept C202751555 @default.
- W2095471664 hasConcept C25642318 @default.
- W2095471664 hasConcept C2777476445 @default.
- W2095471664 hasConcept C2777477808 @default.
- W2095471664 hasConcept C2778043744 @default.
- W2095471664 hasConcept C27881333 @default.
- W2095471664 hasConcept C29730261 @default.
- W2095471664 hasConcept C501593827 @default.
- W2095471664 hasConcept C538909803 @default.
- W2095471664 hasConcept C55493867 @default.
- W2095471664 hasConcept C62231903 @default.
- W2095471664 hasConcept C79879829 @default.
- W2095471664 hasConcept C87644729 @default.
- W2095471664 hasConceptScore W2095471664C178790620 @default.
- W2095471664 hasConceptScore W2095471664C181199279 @default.
- W2095471664 hasConceptScore W2095471664C185592680 @default.
- W2095471664 hasConceptScore W2095471664C202751555 @default.
- W2095471664 hasConceptScore W2095471664C25642318 @default.
- W2095471664 hasConceptScore W2095471664C2777476445 @default.
- W2095471664 hasConceptScore W2095471664C2777477808 @default.
- W2095471664 hasConceptScore W2095471664C2778043744 @default.
- W2095471664 hasConceptScore W2095471664C27881333 @default.
- W2095471664 hasConceptScore W2095471664C29730261 @default.
- W2095471664 hasConceptScore W2095471664C501593827 @default.
- W2095471664 hasConceptScore W2095471664C538909803 @default.
- W2095471664 hasConceptScore W2095471664C55493867 @default.
- W2095471664 hasConceptScore W2095471664C62231903 @default.
- W2095471664 hasConceptScore W2095471664C79879829 @default.
- W2095471664 hasConceptScore W2095471664C87644729 @default.
- W2095471664 hasLocation W20954716641 @default.
- W2095471664 hasLocation W20954716642 @default.
- W2095471664 hasOpenAccess W2095471664 @default.
- W2095471664 hasPrimaryLocation W20954716641 @default.
- W2095471664 hasRelatedWork W1537037928 @default.
- W2095471664 hasRelatedWork W1566748116 @default.
- W2095471664 hasRelatedWork W1775749144 @default.
- W2095471664 hasRelatedWork W1977344741 @default.
- W2095471664 hasRelatedWork W1982895423 @default.
- W2095471664 hasRelatedWork W1984905958 @default.
- W2095471664 hasRelatedWork W1999796979 @default.
- W2095471664 hasRelatedWork W2000328589 @default.
- W2095471664 hasRelatedWork W2001787114 @default.
- W2095471664 hasRelatedWork W2004397560 @default.