Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095482110> ?p ?o ?g. }
- W2095482110 endingPage "1939" @default.
- W2095482110 startingPage "1930" @default.
- W2095482110 abstract "Metalloenzymes and electron transfer proteins influence the electrochemical properties of metal cofactors by controlling the second-sphere environment of the protein active site. Properties that tune this environment include the dielectric constant, templated charge structure, van der Waals interactions, and hydrogen bonds. By systematically varying the binding of a redox-active ligand with a protein, we can evaluate how these noncovalent interactions alter the electronic structure of the bound metal complex. For this study, we employ the well-characterized avidin−biotin conjugate as the protein−ligand system, and have synthesized solvatochromic biotinylated and desthiobiotinylated iron(II) bipyridine tetracyano complexes ([Fe(BMB)(CN)4]2− (1) and [Fe(DMB)(CN)4]2− (2)). The binding affinities of 1 and 2 with avidin are 3.5 × 107 M−1 and 1.5 × 106 M−1, respectively. The redox potentials of 1 and 2 (333 mV and 330 mV) shift to 193 mV and 203 mV vs Ag/AgCl when the complex is bound to avidin and adsorbed to a monolayer-coated gold electrode. Upon binding to avidin, the MLCT1 band red-shifts 20 nm for 1 and 10 nm for 2. Similarly, the MLCT2 band for 1 red-shifts 7 nm and the band for 2 red-shifts 6 nm. For comparison, the electronic properties of 1 and 2 were investigated in organic solvents, and similar shifts in the MLCT bands and redox potentials were observed. An X-ray crystal structure of 1 bound to avidin was obtained, and molecular dynamics simulations were performed to analyze the protein environment of the protein-bound transition metal complexes. Our studies demonstrate that changes in the binding affinity of a ligand-receptor pair influence the outer-sphere coordination of the ligand, which in turn affects the electronic properties of the bound complex." @default.
- W2095482110 created "2016-06-24" @default.
- W2095482110 creator A5000721117 @default.
- W2095482110 creator A5003818033 @default.
- W2095482110 creator A5007664443 @default.
- W2095482110 creator A5015139711 @default.
- W2095482110 creator A5026490818 @default.
- W2095482110 creator A5045168608 @default.
- W2095482110 creator A5073216266 @default.
- W2095482110 creator A5082900597 @default.
- W2095482110 creator A5089524481 @default.
- W2095482110 date "2009-09-29" @default.
- W2095482110 modified "2023-10-17" @default.
- W2095482110 title "Protein Binding and the Electronic Properties of Iron(II) Complexes: An Electrochemical and Optical Investigation of Outer Sphere Effects" @default.
- W2095482110 cites W1707385548 @default.
- W2095482110 cites W1971826830 @default.
- W2095482110 cites W1975814391 @default.
- W2095482110 cites W1976916999 @default.
- W2095482110 cites W1981521205 @default.
- W2095482110 cites W1983831270 @default.
- W2095482110 cites W1986191025 @default.
- W2095482110 cites W1987220178 @default.
- W2095482110 cites W1988774285 @default.
- W2095482110 cites W1989408099 @default.
- W2095482110 cites W1993922491 @default.
- W2095482110 cites W1994286350 @default.
- W2095482110 cites W1995017064 @default.
- W2095482110 cites W1999684045 @default.
- W2095482110 cites W2006642056 @default.
- W2095482110 cites W2007899730 @default.
- W2095482110 cites W2009485848 @default.
- W2095482110 cites W2015314580 @default.
- W2095482110 cites W2015318741 @default.
- W2095482110 cites W2017162622 @default.
- W2095482110 cites W2017446414 @default.
- W2095482110 cites W2022950330 @default.
- W2095482110 cites W2024183979 @default.
- W2095482110 cites W2025710579 @default.
- W2095482110 cites W2027950614 @default.
- W2095482110 cites W2028758769 @default.
- W2095482110 cites W2029066841 @default.
- W2095482110 cites W2036311595 @default.
- W2095482110 cites W2041340670 @default.
- W2095482110 cites W2048526989 @default.
- W2095482110 cites W2052034051 @default.
- W2095482110 cites W2056303381 @default.
- W2095482110 cites W2060470134 @default.
- W2095482110 cites W2063651343 @default.
- W2095482110 cites W2063771259 @default.
- W2095482110 cites W2068617439 @default.
- W2095482110 cites W2073426494 @default.
- W2095482110 cites W2075683473 @default.
- W2095482110 cites W2080376391 @default.
- W2095482110 cites W2080916701 @default.
- W2095482110 cites W2081945699 @default.
- W2095482110 cites W2082948336 @default.
- W2095482110 cites W2083474417 @default.
- W2095482110 cites W2085976686 @default.
- W2095482110 cites W2093161659 @default.
- W2095482110 cites W2094327761 @default.
- W2095482110 cites W2094694122 @default.
- W2095482110 cites W2104554134 @default.
- W2095482110 cites W2106108899 @default.
- W2095482110 cites W2112388751 @default.
- W2095482110 cites W2114926334 @default.
- W2095482110 cites W2118316865 @default.
- W2095482110 cites W2122199548 @default.
- W2095482110 cites W2131713387 @default.
- W2095482110 cites W2133439000 @default.
- W2095482110 cites W2133587541 @default.
- W2095482110 cites W2135839939 @default.
- W2095482110 cites W2149313525 @default.
- W2095482110 cites W2331129251 @default.
- W2095482110 cites W269759422 @default.
- W2095482110 cites W2949726001 @default.
- W2095482110 cites W4211027922 @default.
- W2095482110 cites W67741758 @default.
- W2095482110 doi "https://doi.org/10.1021/bc900270a" @default.
- W2095482110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19788194" @default.
- W2095482110 hasPublicationYear "2009" @default.
- W2095482110 type Work @default.
- W2095482110 sameAs 2095482110 @default.
- W2095482110 citedByCount "14" @default.
- W2095482110 countsByYear W20954821102012 @default.
- W2095482110 countsByYear W20954821102013 @default.
- W2095482110 countsByYear W20954821102016 @default.
- W2095482110 countsByYear W20954821102017 @default.
- W2095482110 countsByYear W20954821102020 @default.
- W2095482110 countsByYear W20954821102021 @default.
- W2095482110 countsByYear W20954821102023 @default.
- W2095482110 crossrefType "journal-article" @default.
- W2095482110 hasAuthorship W2095482110A5000721117 @default.
- W2095482110 hasAuthorship W2095482110A5003818033 @default.
- W2095482110 hasAuthorship W2095482110A5007664443 @default.
- W2095482110 hasAuthorship W2095482110A5015139711 @default.
- W2095482110 hasAuthorship W2095482110A5026490818 @default.
- W2095482110 hasAuthorship W2095482110A5045168608 @default.
- W2095482110 hasAuthorship W2095482110A5073216266 @default.