Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095530524> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2095530524 endingPage "198" @default.
- W2095530524 startingPage "190" @default.
- W2095530524 abstract "The phenolic compound, 2-hydroxy-4-methoxy benzoic acid (HMBA), is one of the major phytoconstituents of Decalepis arayalpathra (Joseph & Chandra.) Venter, a rare and endemic medicinal plant found in the Western Ghats of India. HMBA has been attributed to possess several biological effects including anti-inflammatory, anti-pyretic, anti-oxidant and anti-diabetic. The present article describes a rapid and sensitive liquid chromatography–tandem mass spectrometric method (HPLC–MS/MS) for the determination of HMBA in rat plasma. In brief, the developed assay involves pre-treatment of the plasma samples by an optimized solid phase extraction method (recoveries for HMBA greater than 90%) followed by chromatographic separation on a Cosmosil C18 (150 mm × 4.6 mm i.d.; 5 μm particle size) analytical column with mobile phase of methanol and 10 mM ammonium formate (95:5 v/v; 0.2% formic acid) delivered at a constant flow rate of 1.0 mL/min. The detection and quantification was performed using an Applied Biosystems Hybrid Q-Trap API 2000 mass spectrometer equipped with electrospray ionization source (ESI) functioning in negative mode. The developed assay was validated as per the US FDA bioanalytical guidelines with the calibration curve linear over the concentration range of 5.05–2019.60 ng/mL (r2 ≥ 0.9936) for HMBA from rat plasma. Further, the validated HPLC–MS/MS method was successfully applied to pharmacokinetic study of HMBA after oral administration of D. arayalpathra tuber extracts to female albino Wistar rats using sparse sampling methodology. Following oral administration, the maximum mean concentration in rat plasma (Cmax −1301.57 ± 128.22 ng/mL) was achieved at 1.5 h (Tmax) and the area under the curve (AUC0−48h) was 8985.02 ± 229.54 ng h/mL. The elimination half-life (t1/2) and terminal elimination rate constant (Kel) were 2.48 h and 0.28 L/h, respectively." @default.
- W2095530524 created "2016-06-24" @default.
- W2095530524 creator A5043920091 @default.
- W2095530524 creator A5064648238 @default.
- W2095530524 creator A5068702139 @default.
- W2095530524 creator A5080898467 @default.
- W2095530524 date "2014-11-01" @default.
- W2095530524 modified "2023-09-27" @default.
- W2095530524 title "A validated HPLC-ESI–MS/MS method for quantification of 2-hydroxy-4-methoxy benzoic acid from rat plasma and its application to pharmacokinetic study using sparse sampling methodology" @default.
- W2095530524 cites W1577653758 @default.
- W2095530524 cites W1673495827 @default.
- W2095530524 cites W1894074801 @default.
- W2095530524 cites W1984282278 @default.
- W2095530524 cites W2032788111 @default.
- W2095530524 cites W2050226999 @default.
- W2095530524 cites W2054180830 @default.
- W2095530524 cites W2066291439 @default.
- W2095530524 cites W2067587401 @default.
- W2095530524 cites W2071424589 @default.
- W2095530524 cites W2103201248 @default.
- W2095530524 cites W2113261696 @default.
- W2095530524 cites W2176918598 @default.
- W2095530524 cites W1967063555 @default.
- W2095530524 doi "https://doi.org/10.1016/j.jpba.2014.08.002" @default.
- W2095530524 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25168218" @default.
- W2095530524 hasPublicationYear "2014" @default.
- W2095530524 type Work @default.
- W2095530524 sameAs 2095530524 @default.
- W2095530524 citedByCount "6" @default.
- W2095530524 countsByYear W20955305242019 @default.
- W2095530524 countsByYear W20955305242020 @default.
- W2095530524 countsByYear W20955305242021 @default.
- W2095530524 crossrefType "journal-article" @default.
- W2095530524 hasAuthorship W2095530524A5043920091 @default.
- W2095530524 hasAuthorship W2095530524A5064648238 @default.
- W2095530524 hasAuthorship W2095530524A5068702139 @default.
- W2095530524 hasAuthorship W2095530524A5080898467 @default.
- W2095530524 hasConcept C112705442 @default.
- W2095530524 hasConcept C119128265 @default.
- W2095530524 hasConcept C137535248 @default.
- W2095530524 hasConcept C162356407 @default.
- W2095530524 hasConcept C178790620 @default.
- W2095530524 hasConcept C179998833 @default.
- W2095530524 hasConcept C185592680 @default.
- W2095530524 hasConcept C194451485 @default.
- W2095530524 hasConcept C23531484 @default.
- W2095530524 hasConcept C2775986502 @default.
- W2095530524 hasConcept C2777379063 @default.
- W2095530524 hasConcept C2778844314 @default.
- W2095530524 hasConcept C31827203 @default.
- W2095530524 hasConcept C40684141 @default.
- W2095530524 hasConcept C43617362 @default.
- W2095530524 hasConcept C4725764 @default.
- W2095530524 hasConcept C71043704 @default.
- W2095530524 hasConcept C71924100 @default.
- W2095530524 hasConcept C98274493 @default.
- W2095530524 hasConceptScore W2095530524C112705442 @default.
- W2095530524 hasConceptScore W2095530524C119128265 @default.
- W2095530524 hasConceptScore W2095530524C137535248 @default.
- W2095530524 hasConceptScore W2095530524C162356407 @default.
- W2095530524 hasConceptScore W2095530524C178790620 @default.
- W2095530524 hasConceptScore W2095530524C179998833 @default.
- W2095530524 hasConceptScore W2095530524C185592680 @default.
- W2095530524 hasConceptScore W2095530524C194451485 @default.
- W2095530524 hasConceptScore W2095530524C23531484 @default.
- W2095530524 hasConceptScore W2095530524C2775986502 @default.
- W2095530524 hasConceptScore W2095530524C2777379063 @default.
- W2095530524 hasConceptScore W2095530524C2778844314 @default.
- W2095530524 hasConceptScore W2095530524C31827203 @default.
- W2095530524 hasConceptScore W2095530524C40684141 @default.
- W2095530524 hasConceptScore W2095530524C43617362 @default.
- W2095530524 hasConceptScore W2095530524C4725764 @default.
- W2095530524 hasConceptScore W2095530524C71043704 @default.
- W2095530524 hasConceptScore W2095530524C71924100 @default.
- W2095530524 hasConceptScore W2095530524C98274493 @default.
- W2095530524 hasLocation W20955305241 @default.
- W2095530524 hasLocation W20955305242 @default.
- W2095530524 hasOpenAccess W2095530524 @default.
- W2095530524 hasPrimaryLocation W20955305241 @default.
- W2095530524 hasRelatedWork W2004432487 @default.
- W2095530524 hasRelatedWork W2021034129 @default.
- W2095530524 hasRelatedWork W2035757008 @default.
- W2095530524 hasRelatedWork W2059341053 @default.
- W2095530524 hasRelatedWork W2082766191 @default.
- W2095530524 hasRelatedWork W2090990910 @default.
- W2095530524 hasRelatedWork W2551393718 @default.
- W2095530524 hasRelatedWork W2604691314 @default.
- W2095530524 hasRelatedWork W2972429458 @default.
- W2095530524 hasRelatedWork W2980563832 @default.
- W2095530524 hasVolume "100" @default.
- W2095530524 isParatext "false" @default.
- W2095530524 isRetracted "false" @default.
- W2095530524 magId "2095530524" @default.
- W2095530524 workType "article" @default.