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• W2095591940 endingPage "2231" @default.
• W2095591940 startingPage "2224" @default.
• W2095591940 abstract "The bacterium Rhizobium meliloti, which forms N2-fixing root nodules on alfalfa, has two distinct malic enzymes; one is NADP+ dependent, while a second has maximal activity when NAD+ is the coenzyme. The diphosphopyridine nucleotide (NAD+)-dependent malic enzyme (DME) is required for symbiotic N2 fixation, likely as part of a pathway for the conversion of C4-dicarboxylic acids to acetyl coenzyme A in N2-fixing bacteroids. Here, we report the cloning and localization of the tme gene (encoding the triphosphopyridine nucleotide [NADP+]-dependent malic enzyme) to a 3.7-kb region. We constructed strains carrying insertions within the tme gene region and showed that the NADP+ -dependent malic enzyme activity peak was absent when extracts from these strains were eluted from a DEAE-cellulose chromatography column. We found that NADP+ -dependent malic enzyme activity was not required for N2 fixation, as tme mutants induced N2-fixing root nodules on alfalfa. Moreover, the apparent NADP+ -dependent malic enzyme activity detected in wild-type (N2-fixing) bacteroids was only 20% of the level detected in free-living cells. Much of that residual bacteroid activity appeared to be due to utilization of NADP+ by DME. The functions of DME and the NADP+ -dependent malic enzyme are discussed in light of the above results and the growth phenotypes of various tme and dme mutants." @default.
• W2095591940 created "2016-06-24" @default.
• W2095591940 creator A5000015314 @default.
• W2095591940 creator A5028472018 @default.
• W2095591940 date "1996-04-01" @default.
• W2095591940 modified "2023-09-26" @default.
• W2095591940 title "NADP+ -dependent malic enzyme of Rhizobium meliloti" @default.
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• W2095591940 doi "https://doi.org/10.1128/jb.178.8.2224-2231.1996" @default.
• W2095591940 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/177929" @default.
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