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- W2095632026 abstract "Abstract Persistently infected (PI) BHK 21 and Vero cell lines were established by infection with wild-type (wt) Tacaribe virus and passage of surviving cells. These cells release defective interfering (DI) Tacaribe virus particles, which caused complete inhibition of plaque formation by wt Tacaribe virions. This interference activity was reduced when the DI virus was previously uv inactivated or treated with antiviral serum, and no interferon-like activity was detected. Cells pretreated with DI virus were only partially resistant to challenge with heterotypic arenaviruses, and no interference was detected with plaque formation by vesicular stomatitis virus. PI-BHK and PI-Vero cells contained Tacaribe virus-specific polypeptides as demonstrated by immunofluorescence, and by immunoprecipitation and analysis by SDS-polyacrylamide gel electrophoresis; the major virus-specific polypeptide observed was related to the N protein. PI-Vero cells were resistant to homologous superinfection and slightly resistant to superinfection by heterotypic arenaviruses, but supported normal plaque formation by vesicular stomatitis virus. Purified DI virus had a polypeptide profile similar to wt Tacaribe virions, although the DI virus released from PI-BHK cells had a nucleoprotein which migrated slower than the nucleoprotein of wt virions. The L and S segments of standard virions were not detected in DI particles from PI-BHK cells; but five discrete smaller RNA species were observed (MW ∼ 1.1−0.2 × 106)." @default.
- W2095632026 created "2016-06-24" @default.
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- W2095632026 date "1980-11-01" @default.
- W2095632026 modified "2023-09-27" @default.
- W2095632026 title "Defective interfering Tacaribe virus and persistently infected cells" @default.
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- W2095632026 doi "https://doi.org/10.1016/0042-6822(80)90288-3" @default.
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