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• W2095694857 abstract "Abstract We have screened chromosome arm 3L for ethyl methanesulfonate−induced mutations that disrupt localization of fluorescently labeled gurken (grk) messenger (m)RNA, whose transport along microtubules establishes both major body axes of the developing Drosophila oocyte. Rapid identification of causative mutations by single-nucleotide polymorphism recombinational mapping and whole-genomic sequencing allowed us to define nine complementation groups affecting grk mRNA localization and other aspects of oogenesis, including alleles of elg1, scaf6, quemao, nudE, Tsc2/gigas, rasp, and Chd5/Wrb, and several null alleles of the armitage Piwi-pathway gene. Analysis of a newly induced kinesin light chain allele shows that kinesin motor activity is required for both efficient grk mRNA localization and oocyte centrosome integrity. We also show that initiation of the dorsoanterior localization of grk mRNA precedes centrosome localization, suggesting that microtubule self-organization contributes to breaking axial symmetry to generate a unique dorsoventral axis." @default.
• W2095694857 created "2016-06-24" @default.
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• W2095694857 date "2014-04-01" @default.
• W2095694857 modified "2023-09-27" @default.
• W2095694857 title "A Genetic Screen Based on<i>in Vivo</i>RNA Imaging Reveals Centrosome-Independent Mechanisms for Localizing<i>gurken</i>Transcripts in<i>Drosophila</i>" @default.
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• W2095694857 doi "https://doi.org/10.1534/g3.114.010462" @default.
• W2095694857 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4059244" @default.
• W2095694857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24531791" @default.
• W2095694857 hasPublicationYear "2014" @default.
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