FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2095765587> ?p ?o ?g. }

• W2095765587 endingPage "M110.006403" @default.
• W2095765587 startingPage "M110.006403" @default.
• W2095765587 abstract "One approach to the functional characterization of the lysosome lies in the use of proteomic methods to identify proteins in subcellular fractions enriched for this organelle. However, distinguishing between true lysosomal residents and proteins from other cofractionating organelles is challenging. To this end, we implemented a quantitative mass spectrometry approach based on the selective decrease in the buoyant density of liver lysosomes that occurs when animals are treated with Triton-WR1339. Liver lysosome-enriched preparations from control and treated rats were fractionated by isopycnic sucrose density gradient centrifugation. Tryptic peptides derived from gradient fractions were reacted with isobaric tag for relative and absolute quantitation eight-plex labeling reagents and analyzed by two-dimensional liquid chromatography matrix-assisted laser desorption ionization time-of-flight MS. Reporter ion intensities were used to generate relative protein distribution profiles across both types of gradients. A distribution index was calculated for each identified protein and used to determine a probability of lysosomal residence by quadratic discriminant analysis. This analysis suggests that several proteins assigned to the lysosome in other proteomics studies are not true lysosomal residents. Conversely, results support lysosomal residency for other proteins that are either not or only tentatively assigned to this location. The density shift for two proteins, Cu/Zn superoxide dismutase and ATP-binding cassette subfamily B (MDR/TAP) member 6, was corroborated by quantitative Western blotting. Additional balance sheet analyses on differential centrifugation fractions revealed that Cu/Zn superoxide dismutase is predominantly cytosolic with a secondary lysosomal localization whereas ATP-binding cassette subfamily B (MDR/TAP) member 6 is predominantly lysosomal. These results establish a quantitative mass spectrometric/subcellular fractionation approach for identification of lysosomal proteins and underscore the necessity of balance sheet analysis for localization studies." @default.
• W2095765587 created "2016-06-24" @default.
• W2095765587 creator A5005292388 @default.
• W2095765587 creator A5009692912 @default.
• W2095765587 creator A5020250071 @default.
• W2095765587 creator A5060007272 @default.
• W2095765587 creator A5070195030 @default.
• W2095765587 creator A5077652405 @default.
• W2095765587 date "2011-04-01" @default.
• W2095765587 modified "2023-10-17" @default.
• W2095765587 title "Classification of Subcellular Location by Comparative Proteomic Analysis of Native and Density-shifted Lysosomes" @default.
• W2095765587 cites W1205458192 @default.
• W2095765587 cites W146759131 @default.
• W2095765587 cites W1527697258 @default.
• W2095765587 cites W1880353132 @default.
• W2095765587 cites W191793640 @default.
• W2095765587 cites W1963819382 @default.
• W2095765587 cites W1965552820 @default.
• W2095765587 cites W1966852441 @default.
• W2095765587 cites W1970094644 @default.
• W2095765587 cites W1975182596 @default.
• W2095765587 cites W1979615468 @default.
• W2095765587 cites W1982453950 @default.
• W2095765587 cites W1984347572 @default.
• W2095765587 cites W1993281102 @default.
• W2095765587 cites W199871210 @default.
• W2095765587 cites W1999556153 @default.
• W2095765587 cites W2005168205 @default.
• W2095765587 cites W2005508070 @default.
• W2095765587 cites W2008035413 @default.
• W2095765587 cites W2011048815 @default.
• W2095765587 cites W2014987608 @default.
• W2095765587 cites W2030677245 @default.
• W2095765587 cites W2032482167 @default.
• W2095765587 cites W2039502252 @default.
• W2095765587 cites W2039921716 @default.
• W2095765587 cites W2041876692 @default.
• W2095765587 cites W2053203314 @default.
• W2095765587 cites W2055226243 @default.
• W2095765587 cites W2059106174 @default.
• W2095765587 cites W2059179399 @default.
• W2095765587 cites W2061033316 @default.
• W2095765587 cites W2062541759 @default.
• W2095765587 cites W2063865643 @default.
• W2095765587 cites W2067888080 @default.
• W2095765587 cites W2069502186 @default.
• W2095765587 cites W2070681075 @default.
• W2095765587 cites W2070736329 @default.
• W2095765587 cites W2071633123 @default.
• W2095765587 cites W2075290711 @default.
• W2095765587 cites W2081735322 @default.
• W2095765587 cites W2093229661 @default.
• W2095765587 cites W2093903089 @default.
• W2095765587 cites W2097462613 @default.
• W2095765587 cites W2101117598 @default.
• W2095765587 cites W2103064512 @default.
• W2095765587 cites W2107058904 @default.
• W2095765587 cites W2111025546 @default.
• W2095765587 cites W2112329697 @default.
• W2095765587 cites W2117878765 @default.
• W2095765587 cites W2120403003 @default.
• W2095765587 cites W2125921982 @default.
• W2095765587 cites W2126444364 @default.
• W2095765587 cites W2126489732 @default.
• W2095765587 cites W2130706354 @default.
• W2095765587 cites W2133606489 @default.
• W2095765587 cites W2134091914 @default.
• W2095765587 cites W2135453161 @default.
• W2095765587 cites W2144942968 @default.
• W2095765587 cites W2150158059 @default.
• W2095765587 cites W2153194381 @default.
• W2095765587 cites W2161408389 @default.
• W2095765587 cites W2170281382 @default.
• W2095765587 cites W4321429512 @default.
• W2095765587 doi "https://doi.org/10.1074/mcp.m110.006403" @default.
• W2095765587 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3069348" @default.
• W2095765587 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21252268" @default.
• W2095765587 hasPublicationYear "2011" @default.
• W2095765587 type Work @default.
• W2095765587 sameAs 2095765587 @default.
• W2095765587 citedByCount "40" @default.
• W2095765587 countsByYear W20957655872012 @default.
• W2095765587 countsByYear W20957655872013 @default.
• W2095765587 countsByYear W20957655872014 @default.
• W2095765587 countsByYear W20957655872015 @default.
• W2095765587 countsByYear W20957655872016 @default.
• W2095765587 countsByYear W20957655872017 @default.
• W2095765587 countsByYear W20957655872018 @default.
• W2095765587 countsByYear W20957655872019 @default.
• W2095765587 countsByYear W20957655872020 @default.
• W2095765587 countsByYear W20957655872021 @default.
• W2095765587 countsByYear W20957655872022 @default.
• W2095765587 countsByYear W20957655872023 @default.
• W2095765587 crossrefType "journal-article" @default.
• W2095765587 hasAuthorship W2095765587A5005292388 @default.
• W2095765587 hasAuthorship W2095765587A5009692912 @default.
• W2095765587 hasAuthorship W2095765587A5020250071 @default.
• W2095765587 hasAuthorship W2095765587A5060007272 @default.

• next