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• W2095909097 abstract "Hibernators in torpor dramatically reduce their metabolic, respiratory, and heart rates and core body temperature. These extreme physiological conditions are frequently and rapidly reversed during the winter hibernation season via endogenous mechanisms. This phenotype must derive from regulated expression of the hibernator's genome; to identify its molecular components, a cDNA subtraction was used to enrich for seasonally upregulated mRNAs in liver of golden-mantled ground squirrels. The relative steady-state levels for seven mRNAs identified by this screen, plus five others, were measured and analyzed for seasonal and stage-specific differences using kinetic RT-PCR. Four mRNAs show seasonal upregulation in which all five winter stages differ significantly from and are higher than summer (alpha2-macroglobulin, apolipoprotein A1, cathepsin H, and thyroxine-binding globulin). One of these mRNAs, alpha2-macroglobulin, varies during the winter stages with significantly lower levels at late torpor. None of the 12 mRNAs increased during torpor. The implications for these newly recognized upregulated mRNAs for hibernation as well as more global issues of maintaining steady-state levels of mRNA during torpor are discussed." @default.
• W2095909097 created "2016-06-24" @default.
• W2095909097 creator A5019887423 @default.
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• W2095909097 date "2002-08-14" @default.
• W2095909097 modified "2023-09-23" @default.
• W2095909097 title "Quantitative assessment of ground squirrel mRNA levels in multiple stages of hibernation." @default.
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• W2095909097 doi "https://doi.org/10.1152/physiolgenomics.00004.2002" @default.
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