FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096008575> ?p ?o ?g. }

• W2096008575 endingPage "84" @default.
• W2096008575 startingPage "1178" @default.
• W2096008575 abstract "The PTEN gene at chromosome 10q23.3 is a tumor-suppressor genethat is inactivated in several types of human tumors. Althoughmutation and homozygous deletion are the most commonmechanisms of PTEN inactivation, promoter methylation and translational modification can also account for PTEN silencing. The aim of this study was to investigate the expression of PTEN protein in primary non-small cell lung cancer (NSCLC) samples and to investigate the promoter methylation status of the gene in a panel of NSCLC cell lines as well as primary tumors.We analyzed PTEN expression by immunohistochemistry in tissue samples from 125 patients with early-stage NSCLC. We also evaluated PTEN promoter methylation status by methylation-specific PCR in 20 microdissected PTEN-negative primary tumors from among the last specimens as well as in a panel of 16 NSCLC cell lines. Western and Northern blotting were performed in the same panel of NSCLC cell lines.Thirty (24%) of the 125 specimens showed a lack of staining for PTEN. PTEN methylation was detected in 7 (35%) of the 20 PTEN-negative NSCLC samples and in none of the 10 PTEN-positive NSCLC samples that were microdissected. Furthermore, PTEN methylation was observed in 11 (69%) of the 16 NSCLC cell lines tested. PTEN mRNA expression was increased in the NCI-H1299 cell line by in vitro treatment with the demethylating agent 5-aza-2'-deoxycytidine. PTEN methylation was well correlated with PTEN expression in NSCLC cell lines by Western and Northern blot (P = 0.025).Although genetic alterations of the PTEN gene are rare in NSCLC, loss of PTEN protein is not an uncommon event in early-stage NSCLC. Lack of PTEN expression may be partially explained by promoter methylation." @default.
• W2096008575 created "2016-06-24" @default.
• W2096008575 creator A5010218106 @default.
• W2096008575 creator A5014235192 @default.
• W2096008575 creator A5023135321 @default.
• W2096008575 creator A5027040281 @default.
• W2096008575 creator A5030914311 @default.
• W2096008575 creator A5039814984 @default.
• W2096008575 creator A5059600515 @default.
• W2096008575 creator A5065839430 @default.
• W2096008575 creator A5080439835 @default.
• W2096008575 creator A5082104259 @default.
• W2096008575 date "2002-05-01" @default.
• W2096008575 modified "2023-10-17" @default.
• W2096008575 title "Lack of PTEN expression in non-small cell lung cancer could be related to promoter methylation." @default.
• W2096008575 cites W1551696649 @default.
• W2096008575 cites W1630547983 @default.
• W2096008575 cites W1935114338 @default.
• W2096008575 cites W1969820174 @default.
• W2096008575 cites W1970201564 @default.
• W2096008575 cites W1973186217 @default.
• W2096008575 cites W1973577899 @default.
• W2096008575 cites W1992506198 @default.
• W2096008575 cites W1993312546 @default.
• W2096008575 cites W1993760399 @default.
• W2096008575 cites W1994465394 @default.
• W2096008575 cites W1998038367 @default.
• W2096008575 cites W2000079492 @default.
• W2096008575 cites W2001413092 @default.
• W2096008575 cites W2015323062 @default.
• W2096008575 cites W2023165372 @default.
• W2096008575 cites W2027811142 @default.
• W2096008575 cites W2043395027 @default.
• W2096008575 cites W2059972525 @default.
• W2096008575 cites W2060739426 @default.
• W2096008575 cites W2074696167 @default.
• W2096008575 cites W2076457965 @default.
• W2096008575 cites W2078689449 @default.
• W2096008575 cites W2095860691 @default.
• W2096008575 cites W2102369096 @default.
• W2096008575 cites W2103067815 @default.
• W2096008575 cites W2110511887 @default.
• W2096008575 cites W2113190292 @default.
• W2096008575 cites W2117905749 @default.
• W2096008575 cites W2122522737 @default.
• W2096008575 cites W2125649123 @default.
• W2096008575 cites W2136454222 @default.
• W2096008575 cites W2145211249 @default.
• W2096008575 cites W2152988906 @default.
• W2096008575 cites W2316897574 @default.
• W2096008575 cites W2328322201 @default.
• W2096008575 cites W2328582512 @default.
• W2096008575 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12006535" @default.
• W2096008575 hasPublicationYear "2002" @default.
• W2096008575 type Work @default.
• W2096008575 sameAs 2096008575 @default.
• W2096008575 citedByCount "152" @default.
• W2096008575 countsByYear W20960085752012 @default.
• W2096008575 countsByYear W20960085752013 @default.
• W2096008575 countsByYear W20960085752014 @default.
• W2096008575 countsByYear W20960085752015 @default.
• W2096008575 countsByYear W20960085752016 @default.
• W2096008575 countsByYear W20960085752017 @default.
• W2096008575 countsByYear W20960085752018 @default.
• W2096008575 countsByYear W20960085752019 @default.
• W2096008575 countsByYear W20960085752020 @default.
• W2096008575 countsByYear W20960085752021 @default.
• W2096008575 countsByYear W20960085752022 @default.
• W2096008575 countsByYear W20960085752023 @default.
• W2096008575 crossrefType "journal-article" @default.
• W2096008575 hasAuthorship W2096008575A5010218106 @default.
• W2096008575 hasAuthorship W2096008575A5014235192 @default.
• W2096008575 hasAuthorship W2096008575A5023135321 @default.
• W2096008575 hasAuthorship W2096008575A5027040281 @default.
• W2096008575 hasAuthorship W2096008575A5030914311 @default.
• W2096008575 hasAuthorship W2096008575A5039814984 @default.
• W2096008575 hasAuthorship W2096008575A5059600515 @default.
• W2096008575 hasAuthorship W2096008575A5065839430 @default.
• W2096008575 hasAuthorship W2096008575A5080439835 @default.
• W2096008575 hasAuthorship W2096008575A5082104259 @default.
• W2096008575 hasConcept C104317684 @default.
• W2096008575 hasConcept C119056186 @default.
• W2096008575 hasConcept C121608353 @default.
• W2096008575 hasConcept C142724271 @default.
• W2096008575 hasConcept C150194340 @default.
• W2096008575 hasConcept C153911025 @default.
• W2096008575 hasConcept C190283241 @default.
• W2096008575 hasConcept C190727270 @default.
• W2096008575 hasConcept C2776256026 @default.
• W2096008575 hasConcept C2777609662 @default.
• W2096008575 hasConcept C2778264664 @default.
• W2096008575 hasConcept C2779277951 @default.
• W2096008575 hasConcept C33288867 @default.
• W2096008575 hasConcept C502942594 @default.
• W2096008575 hasConcept C54355233 @default.
• W2096008575 hasConcept C555283112 @default.
• W2096008575 hasConcept C71924100 @default.
• W2096008575 hasConcept C86554907 @default.

• next