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• W2096019762 abstract "Background: Following antigen recognition, naive T helper (Th; CD4+) cells can differentiate toward one of several effector lineages such as Th1 and Th2; each expressing distinctive transcriptional profiles of cytokine genes. These cytokines eventually instruct the strategy of the immune response. In our search for factors that propagate the transcriptional programs of differentiated Th cells, we previously found that Polycomb group (PcG) proteins, which are known as epigenetic regulators that maintain repressive chromatin states, bind differentially the signature cytokine genes. Unexpectedly, their binding to the Ifng (Interferon-g) in Th1 cells and Il4 (Interleukin-4) in Th2 cells, was correlated with transcriptional activation. Therefore, in this study we aimed to determine the functional role of PcG proteins in the regulation of the expression of the signature cytokine genes.Methods: PcG proteins were knocked down in primary and established murine Th cells using transduction of lentiviruses encoding short hairpin RNAs (shRNAs) directed to Mel-18, Ezh2, Eed and Ring1A, representative of two different PcG complexes. The chromatin structure and the binding activity of PcG proteins and transcription factors at the Ifng promoter were assessed by chromatin immunoprecipitation (ChIP) assays.Results: Downregulation of PcG proteins was consistent with their function as positive regulators of the signature cytokine genes in primary and established Th1 and Th2 cells. Moreover, the PcG protein Mel-18 was necessary to recruit the Th1-lineage specifying transcription factor T-bet, and the T cell receptor (TCR)-inducible transcription factor NFAT1 to the Ifng promoter in Th1 cells. Nevertheless, our results suggest that PcG proteins can function also as conventional transcriptional repressors in Th cells of their known target the Hoxa7 gene.Conclusions: Our data support a model whereby the non-differentially expressed PcG proteins are recruited in a Th-lineage specific manner to their target genes to enforce the maintenance of specific transcriptional programs as transcriptional repressors or activators. Although our results suggest a direct effect of PcG proteins in the regulation of cytokine gene expression, indirect functions cannot be excluded." @default.
• W2096019762 created "2016-06-24" @default.
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• W2096019762 date "2011-05-30" @default.
• W2096019762 modified "2023-10-10" @default.
• W2096019762 title "Dual function of polycomb group proteins in differentiated murine T helper (CD4⁺) cells" @default.
• W2096019762 cites W1967269362 @default.
• W2096019762 cites W1973548013 @default.
• W2096019762 cites W1973549360 @default.
• W2096019762 cites W1975702835 @default.
• W2096019762 cites W1977701102 @default.
• W2096019762 cites W1977836335 @default.
• W2096019762 cites W1978139037 @default.
• W2096019762 cites W1984208534 @default.
• W2096019762 cites W1985364817 @default.
• W2096019762 cites W1998770731 @default.
• W2096019762 cites W2002649342 @default.
• W2096019762 cites W2004729573 @default.
• W2096019762 cites W2006101694 @default.
• W2096019762 cites W2010790480 @default.
• W2096019762 cites W2011736381 @default.
• W2096019762 cites W2011882192 @default.
• W2096019762 cites W2016827757 @default.
• W2096019762 cites W2023332575 @default.
• W2096019762 cites W2025856640 @default.
• W2096019762 cites W2026082697 @default.
• W2096019762 cites W2028003321 @default.
• W2096019762 cites W2032504605 @default.
• W2096019762 cites W2033861867 @default.
• W2096019762 cites W2044118609 @default.
• W2096019762 cites W2044627216 @default.
• W2096019762 cites W2046443974 @default.
• W2096019762 cites W2047143392 @default.
• W2096019762 cites W2050531099 @default.
• W2096019762 cites W2052752381 @default.
• W2096019762 cites W2052998574 @default.
• W2096019762 cites W2053317015 @default.
• W2096019762 cites W2054344840 @default.
• W2096019762 cites W2059012075 @default.
• W2096019762 cites W2060870400 @default.
• W2096019762 cites W2062759109 @default.
• W2096019762 cites W2063682537 @default.
• W2096019762 cites W2066170392 @default.
• W2096019762 cites W2067885308 @default.
• W2096019762 cites W2073869073 @default.
• W2096019762 cites W2076956056 @default.
• W2096019762 cites W2082959814 @default.
• W2096019762 cites W2083103029 @default.
• W2096019762 cites W2083292110 @default.
• W2096019762 cites W2085677283 @default.
• W2096019762 cites W2086085458 @default.
• W2096019762 cites W2088388005 @default.
• W2096019762 cites W2090017665 @default.
• W2096019762 cites W2091151146 @default.
• W2096019762 cites W2092345398 @default.
• W2096019762 cites W2092476087 @default.
• W2096019762 cites W2095002834 @default.
• W2096019762 cites W2103226094 @default.
• W2096019762 cites W2111674154 @default.
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• W2096019762 cites W2116318886 @default.
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• W2096019762 cites W2128283220 @default.
• W2096019762 cites W2136278733 @default.
• W2096019762 cites W2146861159 @default.
• W2096019762 cites W2149718894 @default.
• W2096019762 cites W2153424791 @default.
• W2096019762 cites W2158610627 @default.
• W2096019762 cites W2161841622 @default.
• W2096019762 cites W2168250182 @default.
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• W2096019762 doi "https://doi.org/10.1186/1750-2187-6-5" @default.
• W2096019762 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3127800" @default.
• W2096019762 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21624129" @default.
• W2096019762 hasPublicationYear "2011" @default.
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