Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096061034> ?p ?o ?g. }
- W2096061034 endingPage "706" @default.
- W2096061034 startingPage "698" @default.
- W2096061034 abstract "The biological and ligand-binding properties of recombinant C-terminal cell-binding domains (CBDs) and subdomains of the two large exotoxins, Toxin A (TcdA) and Toxin B (TcdB) expressed by Clostridium difficile were examined in the hemagglutination and Verocytotoxicity neutralization assays and by qualitative affinity chromatography using Sepharose-linked αGal(1,3)βGal(1,4)βGlc as well as the direct electrospray ionization mass spectrometry (ES-MS) assay. These studies revealed that, whereas the full-length TcdA CBD agglutinated rabbit erythrocytes, neutralized TcdA-mediated Vero cell death and bound to αGal(1,3)βGal(1,4)βGlc-derivatized Sepharose, the TcdB CBD was inactive in these functional assays. Moreover, retention by αGal(1,3)βGal(1,4)βGlc-derivatized Sepharose corresponded to the number of available TcdA subdomain ligand-binding sites. By contrast, the ES-MS assays revealed that both the TcdA and TcdB CBD bind to 8-methoxycarbonyloctyl-αGal(1,3)βGal(1,4)βGlc sequences with similar avidities. Additional ES-MS experiments using chemically altered αGal(1,3)βGal(1,4)βGlc sequences also revealed that the TcdA and TcdB CBD will tolerate a fair amount of structural variation in their complementary glycan ligands. Although the studies are consistent with the known ligand-binding properties of the TcdA and TcdB holotoxins, they also revealed subtle heretofore unrecognized functional differences in their receptor recognition properties." @default.
- W2096061034 created "2016-06-24" @default.
- W2096061034 creator A5010548726 @default.
- W2096061034 creator A5013277370 @default.
- W2096061034 creator A5028138921 @default.
- W2096061034 creator A5029998395 @default.
- W2096061034 creator A5031933210 @default.
- W2096061034 creator A5033055721 @default.
- W2096061034 creator A5034843193 @default.
- W2096061034 creator A5040743258 @default.
- W2096061034 creator A5043701724 @default.
- W2096061034 creator A5056309331 @default.
- W2096061034 creator A5090630233 @default.
- W2096061034 date "2008-06-25" @default.
- W2096061034 modified "2023-09-24" @default.
- W2096061034 title "Functional properties of the carboxy-terminal host cell-binding domains of the two toxins, TcdA and TcdB, expressed by Clostridium difficile" @default.
- W2096061034 cites W1581583867 @default.
- W2096061034 cites W1879336500 @default.
- W2096061034 cites W1906111837 @default.
- W2096061034 cites W1968804600 @default.
- W2096061034 cites W1976491510 @default.
- W2096061034 cites W1980037845 @default.
- W2096061034 cites W1987018568 @default.
- W2096061034 cites W1992888087 @default.
- W2096061034 cites W2004681646 @default.
- W2096061034 cites W2009247981 @default.
- W2096061034 cites W2012840701 @default.
- W2096061034 cites W2036564231 @default.
- W2096061034 cites W2055030223 @default.
- W2096061034 cites W2058189914 @default.
- W2096061034 cites W2058201892 @default.
- W2096061034 cites W2069011026 @default.
- W2096061034 cites W2073529249 @default.
- W2096061034 cites W2073535747 @default.
- W2096061034 cites W2080446617 @default.
- W2096061034 cites W2091651582 @default.
- W2096061034 cites W2101233913 @default.
- W2096061034 cites W2102301771 @default.
- W2096061034 cites W2102459023 @default.
- W2096061034 cites W2103754733 @default.
- W2096061034 cites W2111990189 @default.
- W2096061034 cites W2115024117 @default.
- W2096061034 cites W2118523654 @default.
- W2096061034 cites W2119561786 @default.
- W2096061034 cites W2126273483 @default.
- W2096061034 cites W2137720465 @default.
- W2096061034 cites W2160692001 @default.
- W2096061034 cites W2164524378 @default.
- W2096061034 doi "https://doi.org/10.1093/glycob/cwn048" @default.
- W2096061034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18509107" @default.
- W2096061034 hasPublicationYear "2008" @default.
- W2096061034 type Work @default.
- W2096061034 sameAs 2096061034 @default.
- W2096061034 citedByCount "62" @default.
- W2096061034 countsByYear W20960610342012 @default.
- W2096061034 countsByYear W20960610342013 @default.
- W2096061034 countsByYear W20960610342014 @default.
- W2096061034 countsByYear W20960610342015 @default.
- W2096061034 countsByYear W20960610342016 @default.
- W2096061034 countsByYear W20960610342017 @default.
- W2096061034 countsByYear W20960610342018 @default.
- W2096061034 countsByYear W20960610342019 @default.
- W2096061034 crossrefType "journal-article" @default.
- W2096061034 hasAuthorship W2096061034A5010548726 @default.
- W2096061034 hasAuthorship W2096061034A5013277370 @default.
- W2096061034 hasAuthorship W2096061034A5028138921 @default.
- W2096061034 hasAuthorship W2096061034A5029998395 @default.
- W2096061034 hasAuthorship W2096061034A5031933210 @default.
- W2096061034 hasAuthorship W2096061034A5033055721 @default.
- W2096061034 hasAuthorship W2096061034A5034843193 @default.
- W2096061034 hasAuthorship W2096061034A5040743258 @default.
- W2096061034 hasAuthorship W2096061034A5043701724 @default.
- W2096061034 hasAuthorship W2096061034A5056309331 @default.
- W2096061034 hasAuthorship W2096061034A5090630233 @default.
- W2096061034 hasBestOaLocation W20960610341 @default.
- W2096061034 hasConcept C104317684 @default.
- W2096061034 hasConcept C108625454 @default.
- W2096061034 hasConcept C116569031 @default.
- W2096061034 hasConcept C153911025 @default.
- W2096061034 hasConcept C170493617 @default.
- W2096061034 hasConcept C181199279 @default.
- W2096061034 hasConcept C185592680 @default.
- W2096061034 hasConcept C206212055 @default.
- W2096061034 hasConcept C2779881004 @default.
- W2096061034 hasConcept C2994496256 @default.
- W2096061034 hasConcept C501593827 @default.
- W2096061034 hasConcept C547475151 @default.
- W2096061034 hasConcept C55493867 @default.
- W2096061034 hasConcept C66999527 @default.
- W2096061034 hasConcept C86803240 @default.
- W2096061034 hasConcept C89423630 @default.
- W2096061034 hasConcept C97505648 @default.
- W2096061034 hasConceptScore W2096061034C104317684 @default.
- W2096061034 hasConceptScore W2096061034C108625454 @default.
- W2096061034 hasConceptScore W2096061034C116569031 @default.
- W2096061034 hasConceptScore W2096061034C153911025 @default.
- W2096061034 hasConceptScore W2096061034C170493617 @default.
- W2096061034 hasConceptScore W2096061034C181199279 @default.