FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096089653> ?p ?o ?g. }

• W2096089653 abstract "MicroRNAs (miRNAs) are important regulators of gene expression and have been implicated in development, differentiation and pathogenesis. Hundreds of miRNAs have been discovered in mammalian genomes. Approximately 50% of mammalian miRNAs are expressed from introns of protein-coding genes; the primary transcript (pri-miRNA) is therefore assumed to be the host transcript. However, very little is known about the structure of pri-miRNAs expressed from intergenic regions. Here we annotate transcript boundaries of miRNAs in human, mouse and rat genomes using various transcription features. The 5' end of the pri-miRNA is predicted from transcription start sites, CpG islands and 5' CAGE tags mapped in the upstream flanking region surrounding the precursor miRNA (pre-miRNA). The 3' end of the pri-miRNA is predicted based on the mapping of polyA signals, and supported by cDNA/EST and ditags data. The predicted pri-miRNAs are also analyzed for promoter and insulator-associated regulatory regions.We define sets of conserved and non-conserved human, mouse and rat pre-miRNAs using bidirectional BLAST and synteny analysis. Transcription features in their flanking regions are used to demarcate the 5' and 3' boundaries of the pri-miRNAs. The lengths and boundaries of primary transcripts are highly conserved between orthologous miRNAs. A significant fraction of pri-miRNAs have lengths between 1 and 10 kb, with very few introns. We annotate a total of 59 pri-miRNA structures, which include 82 pre-miRNAs. 36 pri-miRNAs are conserved in all 3 species. In total, 18 of the confidently annotated transcripts express more than one pre-miRNA. The upstream regions of 54% of the predicted pri-miRNAs are found to be associated with promoter and insulator regulatory sequences.Little is known about the primary transcripts of intergenic miRNAs. Using comparative data, we are able to identify the boundaries of a significant proportion of human, mouse and rat pri-miRNAs. We confidently predict the transcripts including a total of 77, 58 and 47 human, mouse and rat pre-miRNAs respectively. Our computational annotations provide a basis for subsequent experimental validation of predicted pri-miRNAs." @default.
• W2096089653 created "2016-06-24" @default.
• W2096089653 creator A5001502247 @default.
• W2096089653 creator A5002104149 @default.
• W2096089653 creator A5052303040 @default.
• W2096089653 date "2008-11-27" @default.
• W2096089653 modified "2023-10-10" @default.
• W2096089653 title "Annotation of mammalian primary microRNAs" @default.
• W2096089653 cites W144423133 @default.
• W2096089653 cites W1536020257 @default.
• W2096089653 cites W1916491745 @default.
• W2096089653 cites W1979268646 @default.
• W2096089653 cites W1980269624 @default.
• W2096089653 cites W1985738984 @default.
• W2096089653 cites W2000904576 @default.
• W2096089653 cites W2002995168 @default.
• W2096089653 cites W2012258341 @default.
• W2096089653 cites W2012338758 @default.
• W2096089653 cites W2016964783 @default.
• W2096089653 cites W2019387053 @default.
• W2096089653 cites W2029722028 @default.
• W2096089653 cites W2034835460 @default.
• W2096089653 cites W2038067635 @default.
• W2096089653 cites W2038303173 @default.
• W2096089653 cites W2040851473 @default.
• W2096089653 cites W2049868101 @default.
• W2096089653 cites W2054109341 @default.
• W2096089653 cites W2057692691 @default.
• W2096089653 cites W2066213138 @default.
• W2096089653 cites W2070571588 @default.
• W2096089653 cites W2072155079 @default.
• W2096089653 cites W2072229457 @default.
• W2096089653 cites W2077132193 @default.
• W2096089653 cites W2087966784 @default.
• W2096089653 cites W2095125813 @default.
• W2096089653 cites W2099517697 @default.
• W2096089653 cites W2105071916 @default.
• W2096089653 cites W2106929712 @default.
• W2096089653 cites W2113150125 @default.
• W2096089653 cites W2114703458 @default.
• W2096089653 cites W2121607820 @default.
• W2096089653 cites W2122211092 @default.
• W2096089653 cites W2128674168 @default.
• W2096089653 cites W2133763262 @default.
• W2096089653 cites W2142581455 @default.
• W2096089653 cites W2147835141 @default.
• W2096089653 cites W2149291473 @default.
• W2096089653 cites W2150375453 @default.
• W2096089653 cites W2150536104 @default.
• W2096089653 cites W2151587693 @default.
• W2096089653 cites W2157569278 @default.
• W2096089653 cites W2161149887 @default.
• W2096089653 cites W2161859153 @default.
• W2096089653 cites W2166234597 @default.
• W2096089653 cites W2168230240 @default.
• W2096089653 cites W2171230529 @default.
• W2096089653 cites W2411000180 @default.
• W2096089653 cites W2413886249 @default.
• W2096089653 cites W2428698434 @default.
• W2096089653 cites W2597632520 @default.
• W2096089653 cites W4207034695 @default.
• W2096089653 doi "https://doi.org/10.1186/1471-2164-9-564" @default.
• W2096089653 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2632650" @default.
• W2096089653 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19038026" @default.
• W2096089653 hasPublicationYear "2008" @default.
• W2096089653 type Work @default.
• W2096089653 sameAs 2096089653 @default.
• W2096089653 citedByCount "128" @default.
• W2096089653 countsByYear W20960896532012 @default.
• W2096089653 countsByYear W20960896532013 @default.
• W2096089653 countsByYear W20960896532014 @default.
• W2096089653 countsByYear W20960896532015 @default.
• W2096089653 countsByYear W20960896532016 @default.
• W2096089653 countsByYear W20960896532017 @default.
• W2096089653 countsByYear W20960896532018 @default.
• W2096089653 countsByYear W20960896532019 @default.
• W2096089653 countsByYear W20960896532020 @default.
• W2096089653 countsByYear W20960896532021 @default.
• W2096089653 countsByYear W20960896532022 @default.
• W2096089653 countsByYear W20960896532023 @default.
• W2096089653 crossrefType "journal-article" @default.
• W2096089653 hasAuthorship W2096089653A5001502247 @default.
• W2096089653 hasAuthorship W2096089653A5002104149 @default.
• W2096089653 hasAuthorship W2096089653A5052303040 @default.
• W2096089653 hasBestOaLocation W20960896531 @default.
• W2096089653 hasConcept C104317684 @default.
• W2096089653 hasConcept C138885662 @default.
• W2096089653 hasConcept C141231307 @default.
• W2096089653 hasConcept C145059251 @default.
• W2096089653 hasConcept C179926584 @default.
• W2096089653 hasConcept C197077220 @default.
• W2096089653 hasConcept C41895202 @default.
• W2096089653 hasConcept C53702515 @default.
• W2096089653 hasConcept C54355233 @default.
• W2096089653 hasConcept C54458228 @default.
• W2096089653 hasConcept C67585878 @default.
• W2096089653 hasConcept C67705224 @default.
• W2096089653 hasConcept C70721500 @default.
• W2096089653 hasConcept C86803240 @default.
• W2096089653 hasConcept C94671646 @default.

• next