FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096099391> ?p ?o ?g. }

• W2096099391 endingPage "309" @default.
• W2096099391 startingPage "302" @default.
• W2096099391 abstract "The yeast transcriptional activator Gal4 has long been the prototype for studies of eukaryotic transcription. Gal4 is phosphorylated in the DNA-binding domain (DBD); however, the molecular details and functional significance of this remain unknown. We mutagenized seven potential phosphoserines that lie on the solvent-exposed face of the DBD structure and assessed them for transcriptional activity and DNA binding in vivo. Serine to alanine mutants at positions 22, 47, and 85 show the greatest reduction in promoter occupancy and transcriptional activity at the MEL1 promoter containing a single UASGAL . Substitutions with the phosphomimetic aspartate restored DNA-binding and transcriptional activity at serines 22 and 85, suggesting that they are potential sites of Gal4 phosphorylation in vivo. In contrast, the serine to alanine mutants, except serine 22, were fully proficient for binding to the GAL1-10 promoter, containing multiple UASGAL sites, although they had a reduced ability to activate transcription. Collectively, these data show that at the GAL1-10 promoter, functions of the DBD in transcriptional activation can be uncoupled from roles in promoter binding. We suggest that the serines in the DBD mediate protein-protein contacts with the transcription machinery, leading to stabilization of Gal4 at promoters." @default.
• W2096099391 created "2016-06-24" @default.
• W2096099391 creator A5016794097 @default.
• W2096099391 creator A5019352406 @default.
• W2096099391 creator A5062016181 @default.
• W2096099391 creator A5087713515 @default.
• W2096099391 date "2013-10-25" @default.
• W2096099391 modified "2023-10-13" @default.
• W2096099391 title "Solvent-exposed serines in the Gal4 DNA-binding domain are required for promoter occupancy and transcriptional activationin vivo" @default.
• W2096099391 cites W1507254050 @default.
• W2096099391 cites W1763161027 @default.
• W2096099391 cites W1876405769 @default.
• W2096099391 cites W1961376679 @default.
• W2096099391 cites W1970011172 @default.
• W2096099391 cites W1973148221 @default.
• W2096099391 cites W1975962848 @default.
• W2096099391 cites W1978412020 @default.
• W2096099391 cites W1992338259 @default.
• W2096099391 cites W2015617558 @default.
• W2096099391 cites W2021818985 @default.
• W2096099391 cites W2025435904 @default.
• W2096099391 cites W2025470854 @default.
• W2096099391 cites W2025983519 @default.
• W2096099391 cites W2037579319 @default.
• W2096099391 cites W2038013843 @default.
• W2096099391 cites W2044633101 @default.
• W2096099391 cites W2053666983 @default.
• W2096099391 cites W2056168662 @default.
• W2096099391 cites W2056431359 @default.
• W2096099391 cites W2058851268 @default.
• W2096099391 cites W2066951138 @default.
• W2096099391 cites W2072776553 @default.
• W2096099391 cites W2080113951 @default.
• W2096099391 cites W2082797919 @default.
• W2096099391 cites W2087950896 @default.
• W2096099391 cites W2089107873 @default.
• W2096099391 cites W2098080073 @default.
• W2096099391 cites W2105054593 @default.
• W2096099391 cites W2107553595 @default.
• W2096099391 cites W2115218796 @default.
• W2096099391 cites W2116287534 @default.
• W2096099391 cites W2117557926 @default.
• W2096099391 cites W2119714784 @default.
• W2096099391 cites W2124479692 @default.
• W2096099391 cites W2126440278 @default.
• W2096099391 cites W2129366913 @default.
• W2096099391 cites W2130208743 @default.
• W2096099391 cites W2135869142 @default.
• W2096099391 cites W2137994430 @default.
• W2096099391 cites W2147634528 @default.
• W2096099391 cites W2162204490 @default.
• W2096099391 cites W2170788092 @default.
• W2096099391 cites W45968920 @default.
• W2096099391 doi "https://doi.org/10.1111/1567-1364.12106" @default.
• W2096099391 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24119159" @default.
• W2096099391 hasPublicationYear "2013" @default.
• W2096099391 type Work @default.
• W2096099391 sameAs 2096099391 @default.
• W2096099391 citedByCount "2" @default.
• W2096099391 countsByYear W20960993912022 @default.
• W2096099391 countsByYear W20960993912023 @default.
• W2096099391 crossrefType "journal-article" @default.
• W2096099391 hasAuthorship W2096099391A5016794097 @default.
• W2096099391 hasAuthorship W2096099391A5019352406 @default.
• W2096099391 hasAuthorship W2096099391A5062016181 @default.
• W2096099391 hasAuthorship W2096099391A5087713515 @default.
• W2096099391 hasBestOaLocation W20960993911 @default.
• W2096099391 hasConcept C101762097 @default.
• W2096099391 hasConcept C104317684 @default.
• W2096099391 hasConcept C107824862 @default.
• W2096099391 hasConcept C11960822 @default.
• W2096099391 hasConcept C138885662 @default.
• W2096099391 hasConcept C143065580 @default.
• W2096099391 hasConcept C150194340 @default.
• W2096099391 hasConcept C153911025 @default.
• W2096099391 hasConcept C179926584 @default.
• W2096099391 hasConcept C27153228 @default.
• W2096099391 hasConcept C2775880066 @default.
• W2096099391 hasConcept C2776414213 @default.
• W2096099391 hasConcept C2779856020 @default.
• W2096099391 hasConcept C33987129 @default.
• W2096099391 hasConcept C41895202 @default.
• W2096099391 hasConcept C515207424 @default.
• W2096099391 hasConcept C552990157 @default.
• W2096099391 hasConcept C55493867 @default.
• W2096099391 hasConcept C86339819 @default.
• W2096099391 hasConcept C86803240 @default.
• W2096099391 hasConcept C88045685 @default.
• W2096099391 hasConcept C94966510 @default.
• W2096099391 hasConceptScore W2096099391C101762097 @default.
• W2096099391 hasConceptScore W2096099391C104317684 @default.
• W2096099391 hasConceptScore W2096099391C107824862 @default.
• W2096099391 hasConceptScore W2096099391C11960822 @default.
• W2096099391 hasConceptScore W2096099391C138885662 @default.
• W2096099391 hasConceptScore W2096099391C143065580 @default.
• W2096099391 hasConceptScore W2096099391C150194340 @default.
• W2096099391 hasConceptScore W2096099391C153911025 @default.
• W2096099391 hasConceptScore W2096099391C179926584 @default.

• next