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• W2096214110 endingPage "442" @default.
• W2096214110 startingPage "435" @default.
• W2096214110 abstract "In Percoll purified Leydig cells from mature rat we have demonstrated that the basal testosterone production (9.5 ng/106 Leydig cells/24 h) is increased 10-fold in presence of a saturating amount of hCG (1 IU/mL) and diminished in a dose-related manner when larger concentrations of gonadotropin are used to reach 14 ng/106 Leydig cells for 50 IU of hCG. If 40% (v/v) seminiferous tubule medium (STM) is added together with hCG (1 IU/mL) to the incubation medium, a further increase (62%) of testosterone output is noticed. Obviously, when the testosterone production is low as a consequence of a higher dose of hCG (50 IU/mL), the STM (80%) improves the steroid synthesis five-fold (67.4 ng). Concerning the cytoskeletal components (microtubules, intermediate filaments and microfilaments) which have been examined in presence or absence of hCG and STM, we have found a rearrangement of cytoskeletal elements as well as cell-shape changes in relation with hormonal activity of the cells. The most prominent alterations of cytoskeletal elements have been observed after 24 h of incubation with 1 IU/mL of hCG added together with 80% of STM. The obtained results suggest that paracrine factor(s) presents in STM and acting in synergy with LH/hCG generate(s) the rearrangement of cytoskeletal structures which, in turn, facilitates the availability of cholesterol for the mitochondria and finally enhances the testosterone production in the rat Leydig cells." @default.
• W2096214110 created "2016-06-24" @default.
• W2096214110 creator A5038152487 @default.
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• W2096214110 date "1997-10-01" @default.
• W2096214110 modified "2023-10-17" @default.
• W2096214110 title "Paracrine effect of seminiferous tubule factors on rat Leydig cell testosterone production: Role of cytoskeleton" @default.
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• W2096214110 doi "https://doi.org/10.1016/s0248-4900(97)89314-8" @default.
• W2096214110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9561722" @default.
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