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- W2096313500 abstract "New interesting strategies for plasmid DNA (pDNA) purification were designed, exploiting affinity interactions between amino acids and nucleic acids. The potential application of arginine-based chromatography to purify pDNA has been recently described in our work; however, to achieve higher efficiency and selectivity in arginine affinity chromatography, it is essential to characterize the behaviour of binding/elution of supercoiled (sc) isoforms. In this study, two different strategies based on increased sodium chloride (225-250 mm) or arginine (20-70 mm) stepwise gradients are described to purify sc isoforms. Thus, it was proved that well-defined binding/elution conditions are crucial to enhance the purification performance, resulting in an improvement of the final plasmids yields and transfection efficiency, as this could represent a significant impact on therapeutic applications of the purified sc isoform." @default.
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- W2096313500 date "2009-02-01" @default.
- W2096313500 modified "2023-10-14" @default.
- W2096313500 title "Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification" @default.
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- W2096313500 doi "https://doi.org/10.1002/bmc.1097" @default.
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