FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096352001> ?p ?o ?g. }

• W2096352001 endingPage "2332" @default.
• W2096352001 startingPage "2323" @default.
• W2096352001 abstract "The hepatitis C virus (HCV) NS3 protein possesses both protease and helicase activities and is essential for virus replication and maturation. Specific inhibition of NS3 enzymatic activity can be achieved by antibody binding. Transduction of hepatocytes with encoding cDNA leading to intracellular expression of antibody fragments is expected to terminate HCV replication in infected cells. The objective of the present study was the generation of human antibody fragments that neutralize the viral NS3 helicase activity for gene therapeutic applications and drug design. A human immunoglobulin phage-display library cloned from bone marrow aspirate of patients infected with HCV was used for affinity selection against HCV NS3 helicase. Antibody fragments with high affinity to HCV helicase were isolated. To evaluate the inhibitory potential of isolated single-chain antibody fragments, a helicase-mediated, DNA-unwinding enzymatic assay was developed in ELISA format. Recombinant protein comprising the full-length HCV NS3 helicase domain was expressed in the baculovirus expression system. Recombinant antibodies that inhibit the HCV helicase at nanomolar concentrations, with efficacies ranging from 20 % to complete abrogation of enzymatic unwinding activity, were identified. These antibody fragments may be useful for novel gene therapeutic strategies that employ intracellular immunization and may provide new insights into the design of small molecule inhibitors of essential HCV proteins." @default.
• W2096352001 created "2016-06-24" @default.
• W2096352001 creator A5008452332 @default.
• W2096352001 creator A5043505795 @default.
• W2096352001 creator A5049702449 @default.
• W2096352001 creator A5067635515 @default.
• W2096352001 creator A5073812582 @default.
• W2096352001 date "2003-09-01" @default.
• W2096352001 modified "2023-09-23" @default.
• W2096352001 title "Abrogation of hepatitis C virus NS3 helicase enzymatic activity by recombinant human antibodies" @default.
• W2096352001 cites W127928206 @default.
• W2096352001 cites W1489538658 @default.
• W2096352001 cites W1525759724 @default.
• W2096352001 cites W1549258100 @default.
• W2096352001 cites W1781319048 @default.
• W2096352001 cites W1896010868 @default.
• W2096352001 cites W1928472258 @default.
• W2096352001 cites W1966204321 @default.
• W2096352001 cites W1969845015 @default.
• W2096352001 cites W1969943007 @default.
• W2096352001 cites W1972895358 @default.
• W2096352001 cites W1977011387 @default.
• W2096352001 cites W1983575279 @default.
• W2096352001 cites W1992180542 @default.
• W2096352001 cites W1993159643 @default.
• W2096352001 cites W1996192954 @default.
• W2096352001 cites W1997152817 @default.
• W2096352001 cites W1997843363 @default.
• W2096352001 cites W2006067757 @default.
• W2096352001 cites W2010036724 @default.
• W2096352001 cites W2010962189 @default.
• W2096352001 cites W2011261569 @default.
• W2096352001 cites W2013885208 @default.
• W2096352001 cites W2013982103 @default.
• W2096352001 cites W2015316756 @default.
• W2096352001 cites W2032812303 @default.
• W2096352001 cites W2032962654 @default.
• W2096352001 cites W2041008570 @default.
• W2096352001 cites W2067819264 @default.
• W2096352001 cites W2071424113 @default.
• W2096352001 cites W2072346277 @default.
• W2096352001 cites W2072849848 @default.
• W2096352001 cites W2075659454 @default.
• W2096352001 cites W2080332962 @default.
• W2096352001 cites W2089167315 @default.
• W2096352001 cites W2094590619 @default.
• W2096352001 cites W2099900578 @default.
• W2096352001 cites W2124000798 @default.
• W2096352001 cites W2142230632 @default.
• W2096352001 cites W2149270925 @default.
• W2096352001 cites W2150321725 @default.
• W2096352001 cites W2151898297 @default.
• W2096352001 cites W2160425277 @default.
• W2096352001 cites W2163486922 @default.
• W2096352001 cites W2170700544 @default.
• W2096352001 cites W2171905945 @default.
• W2096352001 cites W2175687390 @default.
• W2096352001 cites W2329979483 @default.
• W2096352001 cites W2331088975 @default.
• W2096352001 cites W4245698779 @default.
• W2096352001 doi "https://doi.org/10.1099/vir.0.19299-0" @default.
• W2096352001 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12917452" @default.
• W2096352001 hasPublicationYear "2003" @default.
• W2096352001 type Work @default.
• W2096352001 sameAs 2096352001 @default.
• W2096352001 citedByCount "20" @default.
• W2096352001 countsByYear W20963520012012 @default.
• W2096352001 countsByYear W20963520012013 @default.
• W2096352001 countsByYear W20963520012016 @default.
• W2096352001 crossrefType "journal-article" @default.
• W2096352001 hasAuthorship W2096352001A5008452332 @default.
• W2096352001 hasAuthorship W2096352001A5043505795 @default.
• W2096352001 hasAuthorship W2096352001A5049702449 @default.
• W2096352001 hasAuthorship W2096352001A5067635515 @default.
• W2096352001 hasAuthorship W2096352001A5073812582 @default.
• W2096352001 hasBestOaLocation W20963520011 @default.
• W2096352001 hasConcept C104317684 @default.
• W2096352001 hasConcept C132379496 @default.
• W2096352001 hasConcept C140704245 @default.
• W2096352001 hasConcept C153911025 @default.
• W2096352001 hasConcept C159047783 @default.
• W2096352001 hasConcept C159654299 @default.
• W2096352001 hasConcept C161223559 @default.
• W2096352001 hasConcept C203014093 @default.
• W2096352001 hasConcept C2522874641 @default.
• W2096352001 hasConcept C2776408679 @default.
• W2096352001 hasConcept C40767141 @default.
• W2096352001 hasConcept C55493867 @default.
• W2096352001 hasConcept C67705224 @default.
• W2096352001 hasConcept C83100098 @default.
• W2096352001 hasConcept C86803240 @default.
• W2096352001 hasConceptScore W2096352001C104317684 @default.
• W2096352001 hasConceptScore W2096352001C132379496 @default.
• W2096352001 hasConceptScore W2096352001C140704245 @default.
• W2096352001 hasConceptScore W2096352001C153911025 @default.
• W2096352001 hasConceptScore W2096352001C159047783 @default.
• W2096352001 hasConceptScore W2096352001C159654299 @default.
• W2096352001 hasConceptScore W2096352001C161223559 @default.

• next