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• W2096367007 startingPage "pdb.ip068221" @default.
• W2096367007 abstract "Dissociated cell cultures of the rodent hippocampus have become a standard model for studying many facets of neural development, including the development of polarity, axonal and dendritic growth, and synapse formation. The cultures are quite homogeneous--∼90% of the cells are pyramidal neurons--and it is relatively easy to express green fluorescent protein (GFP)-tagged proteins by transfection. This article describes the cultures and the key features of the system used to image them. It also includes suggestions on labeling cells with GFP-tagged proteins. It concludes with a discussion of the advantages and disadvantages of this culture system." @default.
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• W2096367007 date "2012-03-01" @default.
• W2096367007 modified "2023-10-18" @default.
• W2096367007 title "General Considerations for Live Imaging of Developing Hippocampal Neurons in Culture" @default.
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• W2096367007 doi "https://doi.org/10.1101/pdb.ip068221" @default.
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