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- W2096424462 abstract "SUMMARY: Accumulation of Cs+ by Chlorella salina was 28-fold greater in cells incubated in the presence than in the absence of 0.5 M-NaCl. An approximate 70% removal of external Cs+ resulted after 15 h incubation of cells with 50 μ;M-CsCl and 0.5 M-NaCl. LiCl also had a stimulatory effect on Cs+ uptake, although mannitol did not. Cs+ influx increased with increasing external NaCl concentration and was maximal between 25-500 mM-NaCl at approximately 4 nmol Cs+ h−1 (106 cells)−1. Little effect on Cs+ uptake resulted from the presence of Mg2+ or Ca2+ or from varying the external pH, and Cs+ was relatively non-toxic towards C. salina. At increasing cell densities (from 4 × 105 to 1 × 107 cells ml+1), decreasing amounts of Cs+ were accumulated per cell although the rate of Cs+ removal from the external medium was still greatest at the higher cell densities examined. Freely suspended C. salina and cell-loaded alginate microbeads accumulated similar levels of Cs+, however, 46% of total Cs+ uptake was attributable to the calcium-alginate matrix in the latter case. When Cs+-loaded cells were subjected to hypoosmotic shock, loss of cellular Cs+ occurred allowing easy Cs+ recovery. This loss exceeded 90% of cellular Cs+ when cells were washed with solutions containing ≤ 50 mM-NaCl between consecutive Cs+ uptake periods; these cells subsequently lost their ability to accumulate large amounts of Cs+. Maximal Cs+ uptake (approximately 85.1% removal after three 15 h incubations) occurred when cells were washed with a solution containing 500 mM-NaCl and 200 mM-KCl between incubations. The relevance of these results to the possible use of C. salina in a salt-dependent biological Cs-removal process is discussed." @default.
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- W2096424462 title "Salt-stimulation of caesium accumulation in the euryhaline green microalga Chlorella salina: potential relevance to the development of a biological Cs-removal process" @default.
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- W2096424462 doi "https://doi.org/10.1099/00221287-139-9-2239" @default.
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