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- W2096437845 abstract "Abstract Camptotheca acuminata Decne is an important medicinal plant that contains various cytotoxic alkaloids, such as camptothecine (CPT) and 10‐hydroxycamptothecine (HCPT). A rapid and sensitive liquid chromatography with fluorescence detection (LC‐FLD) method for the quantification of CPT and HCPT is described. The separation was carried out on a DL‐C l8 column (4.6 × 150 mm, 5 µm), with the mobile phase of acetonitrile–sodium dihydrogen phosphate buffer (10 m m ) using an gradient elution at the flow rate of 0.6 mL/min. The LC‐FLD method was validated for linearity, sensitivity, accuracy and precision, and then used to determine the content of the above components. The lower detection limits of CPT and HCPT were 0.4 and 0.1 ng/mL, respectively. The precision was <1.58% and the mean recovery of the analytes was 96.0–98.6%. The LC‐FLD method was successfully applied to determine CPT and HCPT in real samples including C . acuminate , HCPT injection and rat plasma. Copyright © 2015 John Wiley & Sons, Ltd." @default.
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- W2096437845 date "2015-02-27" @default.
- W2096437845 modified "2023-10-16" @default.
- W2096437845 title "Simultaneous determination of camptothecin and 10-hydroxycamptothecine in the C<i>amptotheca acuminate</i>, its medicinal preparation and in rat plasma by liquid chromatography with fluorescence detection" @default.
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- W2096437845 doi "https://doi.org/10.1002/bmc.3452" @default.
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