FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096487287> ?p ?o ?g. }

• W2096487287 endingPage "825" @default.
• W2096487287 startingPage "817" @default.
• W2096487287 abstract "Kar4p is a transcription factor in Saccharomyces cerevisiae that is required for the expression of karyogamy-specific genes during mating, for the efficient transit from G1 during mitosis, and for essential functions during meiosis. Kar4p exists in two forms: a constitutive slower-migrating form, which predominates during vegetative growth, and a faster-migrating form, which is highly induced by mating pheromone. Transcript mapping of KAR4 revealed that the constitutive mRNA was initiated upstream of two in-frame ATG initiation codons, while the major inducible mRNA originated between them. Thus, the two forms of Kar4p are derived from the translation of alternative transcripts, which possess different AUG initiation codons. Site-directed mutations were constructed to inactivate one or the other of the initiation codons, allowing the expression of the two Kar4p forms separately. At normal levels of expression, the constitutive form of Kar4p did not support wild-type levels of mating. However, the two forms of Kar4p could also be expressed separately from the regulatableGAL1 promoter, and no functional difference was detected when they were expressed at equivalent levels. Pulse-chase experiments showed that the induced form of Kar4p was highly expressed and stable during mating but rapidly turned over in vegetative cells. In contrast, the constitutively expressed longer form showed the same rate of turnover regardless of the growth condition. Furthermore, overexpression of either form of Kar4p in vegetative cells was toxic. Thus, the elaborate regulation of the two forms of Kar4p at the levels of transcription, translation, and protein turnover reflects the requirement for high levels of the protein during mating and for low levels during the subsequent phases of the cell cycle." @default.
• W2096487287 created "2016-06-24" @default.
• W2096487287 creator A5002822104 @default.
• W2096487287 creator A5006467772 @default.
• W2096487287 creator A5066014740 @default.
• W2096487287 creator A5090888692 @default.
• W2096487287 date "1999-01-01" @default.
• W2096487287 modified "2023-10-15" @default.
• W2096487287 title "The Two Forms of Karyogamy Transcription Factor Kar4p Are Regulated by Differential Initiation of Transcription, Translation, and Protein Turnover" @default.
• W2096487287 cites W1526504444 @default.
• W2096487287 cites W1553490318 @default.
• W2096487287 cites W1573328658 @default.
• W2096487287 cites W1573917001 @default.
• W2096487287 cites W1605707346 @default.
• W2096487287 cites W1749612483 @default.
• W2096487287 cites W1966263533 @default.
• W2096487287 cites W1967765244 @default.
• W2096487287 cites W1968606500 @default.
• W2096487287 cites W1969859852 @default.
• W2096487287 cites W1973275541 @default.
• W2096487287 cites W2003771584 @default.
• W2096487287 cites W2014000795 @default.
• W2096487287 cites W2017996587 @default.
• W2096487287 cites W2021329972 @default.
• W2096487287 cites W2028070789 @default.
• W2096487287 cites W2039038110 @default.
• W2096487287 cites W2041770258 @default.
• W2096487287 cites W2051373532 @default.
• W2096487287 cites W2060484288 @default.
• W2096487287 cites W2067397983 @default.
• W2096487287 cites W2071056869 @default.
• W2096487287 cites W2072281880 @default.
• W2096487287 cites W2074126694 @default.
• W2096487287 cites W2080273350 @default.
• W2096487287 cites W2085816567 @default.
• W2096487287 cites W2089155710 @default.
• W2096487287 cites W2093647300 @default.
• W2096487287 cites W2098359644 @default.
• W2096487287 cites W2112203177 @default.
• W2096487287 cites W2121781216 @default.
• W2096487287 cites W2122412269 @default.
• W2096487287 cites W2123527149 @default.
• W2096487287 cites W2158664291 @default.
• W2096487287 cites W2169333620 @default.
• W2096487287 cites W2191877481 @default.
• W2096487287 cites W4247817302 @default.
• W2096487287 doi "https://doi.org/10.1128/mcb.19.1.817" @default.
• W2096487287 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/83938" @default.
• W2096487287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9858604" @default.
• W2096487287 hasPublicationYear "1999" @default.
• W2096487287 type Work @default.
• W2096487287 sameAs 2096487287 @default.
• W2096487287 citedByCount "39" @default.
• W2096487287 countsByYear W20964872872012 @default.
• W2096487287 countsByYear W20964872872013 @default.
• W2096487287 countsByYear W20964872872014 @default.
• W2096487287 countsByYear W20964872872015 @default.
• W2096487287 countsByYear W20964872872016 @default.
• W2096487287 countsByYear W20964872872018 @default.
• W2096487287 countsByYear W20964872872019 @default.
• W2096487287 countsByYear W20964872872020 @default.
• W2096487287 countsByYear W20964872872021 @default.
• W2096487287 countsByYear W20964872872023 @default.
• W2096487287 crossrefType "journal-article" @default.
• W2096487287 hasAuthorship W2096487287A5002822104 @default.
• W2096487287 hasAuthorship W2096487287A5006467772 @default.
• W2096487287 hasAuthorship W2096487287A5066014740 @default.
• W2096487287 hasAuthorship W2096487287A5090888692 @default.
• W2096487287 hasBestOaLocation W20964872871 @default.
• W2096487287 hasConcept C104317684 @default.
• W2096487287 hasConcept C105580179 @default.
• W2096487287 hasConcept C138885662 @default.
• W2096487287 hasConcept C149364088 @default.
• W2096487287 hasConcept C150194340 @default.
• W2096487287 hasConcept C176990463 @default.
• W2096487287 hasConcept C179926584 @default.
• W2096487287 hasConcept C198348125 @default.
• W2096487287 hasConcept C2777576037 @default.
• W2096487287 hasConcept C41895202 @default.
• W2096487287 hasConcept C4718897 @default.
• W2096487287 hasConcept C54355233 @default.
• W2096487287 hasConcept C86339819 @default.
• W2096487287 hasConcept C86803240 @default.
• W2096487287 hasConcept C93304396 @default.
• W2096487287 hasConcept C95444343 @default.
• W2096487287 hasConceptScore W2096487287C104317684 @default.
• W2096487287 hasConceptScore W2096487287C105580179 @default.
• W2096487287 hasConceptScore W2096487287C138885662 @default.
• W2096487287 hasConceptScore W2096487287C149364088 @default.
• W2096487287 hasConceptScore W2096487287C150194340 @default.
• W2096487287 hasConceptScore W2096487287C176990463 @default.
• W2096487287 hasConceptScore W2096487287C179926584 @default.
• W2096487287 hasConceptScore W2096487287C198348125 @default.
• W2096487287 hasConceptScore W2096487287C2777576037 @default.
• W2096487287 hasConceptScore W2096487287C41895202 @default.
• W2096487287 hasConceptScore W2096487287C4718897 @default.
• W2096487287 hasConceptScore W2096487287C54355233 @default.
• W2096487287 hasConceptScore W2096487287C86339819 @default.

• next