Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096497223> ?p ?o ?g. }
- W2096497223 abstract "Faecal pollution on bathing beaches poses a potential threat to human health and as a result may also negatively affect the local economy. In instances where the source of such pollution is not obvious, it may be necessary to track such sources using a host-specific genetic markers technique. Bacteroides species are potential indicators for source tracking of faecal pollution in bathing waters. This study designed specific primer sets to amplify sections of the 16S rRNA gene unique to Bacteroides from domestic dogs and used quantitative PCR (qPCR) to quantify such genetic markers in environmental samples. The sensitivity and specificity of the primer sets was determined; they were specific in silico against known dog Bacteroides sequences and in vitro against Bacteroides sequences originating from human and livestock faeces. Dog faecal Bacteroides contamination was then detected in sea water during the bathing season at a local beach where dogs are banned during the summer months, in spite of the fact that these waters had met EU directive standards based on the culture-based enumeration of faecal indicator bacteria. Quantitative PCR was used to determine the limit of detection (LOD) of the dog Bacteroides genetic markers in these water samples. The copy number of dog Bacteroides genetic markers in the water was low and the LOD of those markers was 4 copies per reaction. The use of these dog primers has the potential to supply important additional information when source tracking faecal pollution at bathing beaches and maintaining water quality." @default.
- W2096497223 created "2016-06-24" @default.
- W2096497223 creator A5063440188 @default.
- W2096497223 creator A5066616038 @default.
- W2096497223 date "2014-01-01" @default.
- W2096497223 modified "2023-10-01" @default.
- W2096497223 title "Development and use of Bacteroides 16S rRNA Polymerase Chain Reaction Assay for Source Tracking Dog Faecal Pollution in Bathing Waters" @default.
- W2096497223 cites W1773063847 @default.
- W2096497223 cites W1777269504 @default.
- W2096497223 cites W1916627165 @default.
- W2096497223 cites W1973200293 @default.
- W2096497223 cites W1973954044 @default.
- W2096497223 cites W1981675027 @default.
- W2096497223 cites W1984971020 @default.
- W2096497223 cites W1987375372 @default.
- W2096497223 cites W1988053043 @default.
- W2096497223 cites W1989324089 @default.
- W2096497223 cites W2006465310 @default.
- W2096497223 cites W2015082859 @default.
- W2096497223 cites W2016238746 @default.
- W2096497223 cites W2024881964 @default.
- W2096497223 cites W2043986538 @default.
- W2096497223 cites W2045548030 @default.
- W2096497223 cites W2060257436 @default.
- W2096497223 cites W2073415403 @default.
- W2096497223 cites W2074539582 @default.
- W2096497223 cites W2082079543 @default.
- W2096497223 cites W2082162718 @default.
- W2096497223 cites W2099651326 @default.
- W2096497223 cites W2100171490 @default.
- W2096497223 cites W2102555068 @default.
- W2096497223 cites W2104688104 @default.
- W2096497223 cites W2115446098 @default.
- W2096497223 cites W2117493970 @default.
- W2096497223 cites W2118482166 @default.
- W2096497223 cites W2118792406 @default.
- W2096497223 cites W2119373645 @default.
- W2096497223 cites W2119565384 @default.
- W2096497223 cites W2120477141 @default.
- W2096497223 cites W2121409138 @default.
- W2096497223 cites W2128277876 @default.
- W2096497223 cites W2133771437 @default.
- W2096497223 cites W2135273412 @default.
- W2096497223 cites W2144775551 @default.
- W2096497223 cites W2146713330 @default.
- W2096497223 cites W2150716871 @default.
- W2096497223 cites W2161240805 @default.
- W2096497223 cites W2161388682 @default.
- W2096497223 cites W2166744717 @default.
- W2096497223 cites W2168420558 @default.
- W2096497223 cites W2169247063 @default.
- W2096497223 cites W2170638721 @default.
- W2096497223 doi "https://doi.org/10.4172/2157-7587.1000163" @default.
- W2096497223 hasPublicationYear "2014" @default.
- W2096497223 type Work @default.
- W2096497223 sameAs 2096497223 @default.
- W2096497223 citedByCount "5" @default.
- W2096497223 countsByYear W20964972232018 @default.
- W2096497223 countsByYear W20964972232020 @default.
- W2096497223 countsByYear W20964972232022 @default.
- W2096497223 countsByYear W20964972232023 @default.
- W2096497223 crossrefType "journal-article" @default.
- W2096497223 hasAuthorship W2096497223A5063440188 @default.
- W2096497223 hasAuthorship W2096497223A5066616038 @default.
- W2096497223 hasBestOaLocation W20964972231 @default.
- W2096497223 hasConcept C104317684 @default.
- W2096497223 hasConcept C107872376 @default.
- W2096497223 hasConcept C124956284 @default.
- W2096497223 hasConcept C136764020 @default.
- W2096497223 hasConcept C185592680 @default.
- W2096497223 hasConcept C18903297 @default.
- W2096497223 hasConcept C2778168646 @default.
- W2096497223 hasConcept C2779167275 @default.
- W2096497223 hasConcept C2780797713 @default.
- W2096497223 hasConcept C39432304 @default.
- W2096497223 hasConcept C41008148 @default.
- W2096497223 hasConcept C42062724 @default.
- W2096497223 hasConcept C49105822 @default.
- W2096497223 hasConcept C521259446 @default.
- W2096497223 hasConcept C523546767 @default.
- W2096497223 hasConcept C54355233 @default.
- W2096497223 hasConcept C55493867 @default.
- W2096497223 hasConcept C86803240 @default.
- W2096497223 hasConcept C89423630 @default.
- W2096497223 hasConceptScore W2096497223C104317684 @default.
- W2096497223 hasConceptScore W2096497223C107872376 @default.
- W2096497223 hasConceptScore W2096497223C124956284 @default.
- W2096497223 hasConceptScore W2096497223C136764020 @default.
- W2096497223 hasConceptScore W2096497223C185592680 @default.
- W2096497223 hasConceptScore W2096497223C18903297 @default.
- W2096497223 hasConceptScore W2096497223C2778168646 @default.
- W2096497223 hasConceptScore W2096497223C2779167275 @default.
- W2096497223 hasConceptScore W2096497223C2780797713 @default.
- W2096497223 hasConceptScore W2096497223C39432304 @default.
- W2096497223 hasConceptScore W2096497223C41008148 @default.
- W2096497223 hasConceptScore W2096497223C42062724 @default.
- W2096497223 hasConceptScore W2096497223C49105822 @default.
- W2096497223 hasConceptScore W2096497223C521259446 @default.
- W2096497223 hasConceptScore W2096497223C523546767 @default.
- W2096497223 hasConceptScore W2096497223C54355233 @default.