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- W2096526725 abstract "Electrophoresis of large linear T2 (162 kbp) and 3-arm star-branched (NArm = 48.5 kbp) DNA in linear polyacrylamide (LPA) solutions above the overlap concentration c* has been investigated using a fluorescence visualization technique that allows both the conformation and mobility μ of the DNA to be determined. LPA solutions of moderate polydispersity index (PI ∼ 1.7–2.1) and variable polymer molecular weight Mw (0.59–2.05 MDa) are used as the sieving media. In unentangled semidilute solutions (c*<c<ce), we find that the conformational dynamics of linear and star-branched DNA in electric fields are strikingly different; the former migrating in predominantly U- or I-shaped conformations, depending on electric field strength E, and the latter migrating in a squid-like profile with the star-arms outstretched in the direction opposite to E and dragging the branch point through the sieving medium. Despite these visual differences, μ for linear and star-branched DNA of comparable size are found to be nearly identical in semidilute, unentangled LPA solutions. For LPA concentrations above the entanglement threshold (c>ce), the conformation of migrating linear and star-shaped DNA manifest only subtle changes from their unentangled solution features, but μ for the stars decreases strongly with increasing LPA concentration and molecular weight, while μ for linear DNA becomes nearly independent of c and Mw. These findings are discussed in the context of current theories for electrophoresis of large polyelectrolytes." @default.
- W2096526725 created "2016-06-24" @default.
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- W2096526725 date "2006-08-01" @default.
- W2096526725 modified "2023-09-23" @default.
- W2096526725 title "Electrophoretic mobility of linear and star-branched DNA in semidilute polymer solutions" @default.
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- W2096526725 doi "https://doi.org/10.1002/elps.200500836" @default.
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