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- W2096562034 abstract "ABSTRACT The β-core fragment of human chorionic gonadotrophin (hCG) is a major part of the immunoreactive hCG-like material found in the urine of normal pregnant women. Patients with non-trophoblastic gynaecological malignancies have been found to have raised levels of urinary β-core. We describe the purification of β-core, the preparation of a polyclonal sheep antiserum and the development of radioimmunoassay. The minimum detection limit of this assay was 0·025 μg β-core/l. There was no significant cross-reaction with the free α-subunit, hLH, hFSH and hTSH (<0·7%), and only partial cross-reaction with intact hCG and free β-subunit of hCG (6·9 and 18%). Within-assay variability ranged from 2·03 to 12·5% and between-assay variability from 2·25 to 13·4%. The assay was applied to urine samples from 92 normal non-pregnant premenopausal women, 54 normal postmenopausal women and 65 women with active gynaecological disease (47 postmenopausal and 18 premenopausal). In normal premenopausal women the values ranged from < 0·025 to 0·62 μg β-core/l (median 0·043 μg β-core/l). The values for normal postmenopausal women ranged from <0·025 to 0·64 μg β-core/l (median 0·26 μg β-core/l). Postmenopausal women with gynaecological malignancy had values which ranged from <0·025 to 4·0 μg/β-core/l (median 0·31 μg β-core/l); premenopausal women in this group had values which ranged from < 0·025 to 1·15 μg β-core/l (median 0·12 μg β-core/l). On molecular sieve chromatography, the material found in the urine of normal postmenopausal women showed the physicochemical characteristics of authentic β-core. However, the only detectable serum gonadotrophin was LH. Considering the homology and evolution of hCG from LH, postmenopausal β-core-like material could be derived from the degradation of β-LH resulting in a fragment with essentially identical physicochemical and immunological properties. The use of β-core assay as a tumour marker must take account of the fact that this molecule occurs in the urine of normal subjects, and that the levels are substantially raised in normal postmenopausal women. Journal of Endocrinology (1991) 130, 481–489" @default.
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- W2096562034 date "1991-09-01" @default.
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- W2096562034 title "The purification and development of a radioimmunoassay for β-core fragment of human chorionic gonadotrophin in urine: application as a marker of gynaecological cancer in premenopausal and postmenopausal women" @default.
- W2096562034 doi "https://doi.org/10.1677/joe.0.1300481" @default.
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