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• W2096576245 endingPage "96" @default.
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• W2096576245 abstract "BHMT (betaine–homocysteine methyltransferase) remethylates homocysteine to form methionine. SAM (S-adenosylmethionine) inhibits BHMT activity, but whether SAM modulates BHMT gene expression is unknown. Transcriptional regulation of the human BHMT is also unknown. The present study examined regulation of the human BHMT gene by SAM and its metabolite, MTA (5′-methylthioadenosine). To facilitate these studies, we cloned the 2.7 kb 5′-flanking region of the human BHMT gene (GenBank® accession number AY325901). Both SAM and MTA treatment of HepG2 cells resulted in a dose- and time-dependent decrease in BHMT mRNA levels, which paralleled their effects on the BHMT promoter activity. Maximal suppression was observed with the BHMT promoter construct −347/+33, which contains a number of NF-κB (nuclear factor κB) binding sites. SAM and MTA treatment increased NF-κB nuclear binding and NF-κB-driven luciferase activities, and increased nuclear binding activity of multiple histone deacetylase co-repressors to the NF-κB sites. Overexpression of p50 and p65 decreased BHMT promoter activity, while blocking NF-κB activation increased BHMT expression and promoter activity, and prevented SAM but not MTA's ability to inhibit BHMT expression. The NF-κB binding site at −301 is responsible, at least in part, for this effect. Lower BHMT expression can impair homocysteine metabolism, which can induce ER (endoplasmic reticulum) stress. Indeed, MTA treatment resulted in increased expression ER stress markers. In conclusion, SAM and MTA down-regulate BHMT expression in HepG2 cells in part by inducing NF-κB, which acts as a repressor for the human BHMT gene. While SAM's mechanism is NF-κB-dependent, MTA has both NF-κB-dependent and -independent mechanisms." @default.
• W2096576245 created "2016-06-24" @default.
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• W2096576245 date "2006-12-11" @default.
• W2096576245 modified "2023-10-01" @default.
• W2096576245 title "Inhibition of human betaine–homocysteine methyltransferase expression by <i>S</i>-adenosylmethionine and methylthioadenosine" @default.
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• W2096576245 doi "https://doi.org/10.1042/bj20061119" @default.
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