FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096783657> ?p ?o ?g. }

• W2096783657 endingPage "120" @default.
• W2096783657 startingPage "112" @default.
• W2096783657 abstract "Adrenomedullin (ADM) in the brain plays important roles in the maintenance of homeostasis. Although in vivo evidence has suggested that nitric oxide (NO) mediates ADM9s effects in the brain, mechanisms for ADM stimulation of NO production in neurons have not been identified. In the present study, primary hypothalamic neurons were used to characterize ADM-induced NO production and to study the underlying mechanisms. Using Calcium Orange/4-amino-5-methylamino-2′,7′-difluorofluorescein fluorescence live cell imaging, we found that ADM (1 or 10 nM, 5 min) significantly elevated [Ca²⁺]_i and NO production in a concentration-dependent manner. Ca²⁺ and NO responses induced by 10 nM ADM were abolished by pretreatment with 50 μM 1,2-bis(2-aminophenoxy)ethane-<i>N</i>,<i>N</i>,<i>N</i>′,<i>N</i>′-tetraacetic acid-acetoxymethyl ester (BAPTA-AM), an intracellular Ca²⁺ chelator, or protein kinase A (PKA) inhibitors 5 μM <i>N</i>-[2-(<i>p</i>-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H-89) and 50 μM Rp-cAMP. Furthermore, the ADM-induced NO production was significantly attenuated by a protein phosphatase 1/2A inhibitor, okadaic acid (OA; 0.1 μM), or calcineurin inhibitors, tacrolimus (FK506) (1 μM) and cyclosporin A (CsA; 0.1 μM). Using Western blotting, we found that ADM significantly decreased phosphorylation of neuronal nitric-oxide synthase (nNOS) at serine 847. This dephosphorylation was inhibited by 0.1 μM OA, 1 μM FK506, 0.1 μM CsA, or 5 μM H-89, and attenuated by 50 μM BAPTA-AM. These results suggest that, in hypothalamic neurons, ADM elevates [Ca²⁺]_i via PKA-associated mechanisms. The PKA/Ca²⁺ cascade leads to protein phosphatase (PP) 1/PP2A- and calcineurin-mediated dephosphorylation of nNOS. We hypothesize that the Ca²⁺ increase and nNOS dephosphorylation contribute to activation of nNOS and production of NO in hypothalamic neurons." @default.
• W2096783657 created "2016-06-24" @default.
• W2096783657 creator A5045319653 @default.
• W2096783657 creator A5054913350 @default.
• W2096783657 date "2007-04-19" @default.
• W2096783657 modified "2023-09-23" @default.
• W2096783657 title "Adrenomedullin Stimulates Nitric Oxide Production from Primary Rat Hypothalamic Neurons: Roles of Calcium and Phosphatases" @default.
• W2096783657 cites W1517888291 @default.
• W2096783657 cites W1559164371 @default.
• W2096783657 cites W1570659420 @default.
• W2096783657 cites W1584255678 @default.
• W2096783657 cites W1602807836 @default.
• W2096783657 cites W1646747014 @default.
• W2096783657 cites W1972828509 @default.
• W2096783657 cites W1973531983 @default.
• W2096783657 cites W1974109612 @default.
• W2096783657 cites W1975416249 @default.
• W2096783657 cites W1993904794 @default.
• W2096783657 cites W2005209827 @default.
• W2096783657 cites W2006276182 @default.
• W2096783657 cites W2017234110 @default.
• W2096783657 cites W2028734020 @default.
• W2096783657 cites W2030693468 @default.
• W2096783657 cites W2033880803 @default.
• W2096783657 cites W2038261669 @default.
• W2096783657 cites W2038563834 @default.
• W2096783657 cites W2040470787 @default.
• W2096783657 cites W2057250100 @default.
• W2096783657 cites W2063453191 @default.
• W2096783657 cites W2068403366 @default.
• W2096783657 cites W2068488670 @default.
• W2096783657 cites W2074295150 @default.
• W2096783657 cites W2076069411 @default.
• W2096783657 cites W2078304896 @default.
• W2096783657 cites W2092390266 @default.
• W2096783657 cites W2092419989 @default.
• W2096783657 cites W2104549499 @default.
• W2096783657 cites W2111112233 @default.
• W2096783657 cites W2111165009 @default.
• W2096783657 cites W2113408108 @default.
• W2096783657 cites W2150186313 @default.
• W2096783657 cites W2170181181 @default.
• W2096783657 cites W4229906588 @default.
• W2096783657 doi "https://doi.org/10.1124/mol.106.033761" @default.
• W2096783657 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17446268" @default.
• W2096783657 hasPublicationYear "2007" @default.
• W2096783657 type Work @default.
• W2096783657 sameAs 2096783657 @default.
• W2096783657 citedByCount "27" @default.
• W2096783657 countsByYear W20967836572012 @default.
• W2096783657 countsByYear W20967836572013 @default.
• W2096783657 countsByYear W20967836572015 @default.
• W2096783657 countsByYear W20967836572016 @default.
• W2096783657 countsByYear W20967836572017 @default.
• W2096783657 countsByYear W20967836572020 @default.
• W2096783657 countsByYear W20967836572021 @default.
• W2096783657 countsByYear W20967836572022 @default.
• W2096783657 countsByYear W20967836572023 @default.
• W2096783657 crossrefType "journal-article" @default.
• W2096783657 hasAuthorship W2096783657A5045319653 @default.
• W2096783657 hasAuthorship W2096783657A5054913350 @default.
• W2096783657 hasConcept C11960822 @default.
• W2096783657 hasConcept C126322002 @default.
• W2096783657 hasConcept C128057223 @default.
• W2096783657 hasConcept C134018914 @default.
• W2096783657 hasConcept C170493617 @default.
• W2096783657 hasConcept C178666793 @default.
• W2096783657 hasConcept C178790620 @default.
• W2096783657 hasConcept C181912034 @default.
• W2096783657 hasConcept C185592680 @default.
• W2096783657 hasConcept C195794163 @default.
• W2096783657 hasConcept C2776108454 @default.
• W2096783657 hasConcept C2776953658 @default.
• W2096783657 hasConcept C2777152744 @default.
• W2096783657 hasConcept C2777622882 @default.
• W2096783657 hasConcept C2911091166 @default.
• W2096783657 hasConcept C519063684 @default.
• W2096783657 hasConcept C519581460 @default.
• W2096783657 hasConcept C55493867 @default.
• W2096783657 hasConcept C71924100 @default.
• W2096783657 hasConcept C86803240 @default.
• W2096783657 hasConceptScore W2096783657C11960822 @default.
• W2096783657 hasConceptScore W2096783657C126322002 @default.
• W2096783657 hasConceptScore W2096783657C128057223 @default.
• W2096783657 hasConceptScore W2096783657C134018914 @default.
• W2096783657 hasConceptScore W2096783657C170493617 @default.
• W2096783657 hasConceptScore W2096783657C178666793 @default.
• W2096783657 hasConceptScore W2096783657C178790620 @default.
• W2096783657 hasConceptScore W2096783657C181912034 @default.
• W2096783657 hasConceptScore W2096783657C185592680 @default.
• W2096783657 hasConceptScore W2096783657C195794163 @default.
• W2096783657 hasConceptScore W2096783657C2776108454 @default.
• W2096783657 hasConceptScore W2096783657C2776953658 @default.
• W2096783657 hasConceptScore W2096783657C2777152744 @default.
• W2096783657 hasConceptScore W2096783657C2777622882 @default.
• W2096783657 hasConceptScore W2096783657C2911091166 @default.
• W2096783657 hasConceptScore W2096783657C519063684 @default.
• W2096783657 hasConceptScore W2096783657C519581460 @default.

• next