Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096787468> ?p ?o ?g. }
- W2096787468 endingPage "e19331" @default.
- W2096787468 startingPage "e19331" @default.
- W2096787468 abstract "Enterohemorrhagic Escherichia coli (EHEC) O157:H7 causes hemorrhagic colitis and may result in potentially fatal hemolytic uremia syndrome in humans. EHEC colonize the intestinal mucosa and promote the formation of actin-rich pedestals via translocated type III effectors. Two EHEC type III secreted effectors, Tir and EspFu/TccP, are key players for pedestal formation. We discovered that an EHEC effector protein called Non-LEE-encoded Ligase (NleL) is an E3 ubiquitin ligase. In vitro, we showed that the NleL C753 residue is critical for its E3 ligase activity. Functionally, we demonstrated that NleL E3 ubiquitin ligase activity is involved in modulating Tir-mediated pedestal formation. Surprisingly, EHEC mutant strain deficient in the E3 ligase activity induced more pedestals than the wild-type strain. The canonical EPEC strain E2348/69 normally lacks the nleL gene, and the ectopic expression of the wild-type EHEC nleL, but not the catalytically-deficient nleL(C753A) mutant, in this strain resulted in fewer actin-rich pedestals. Furthermore, we showed that the C. rodentium NleL homolog is a E3 ubiquitin ligase and is required for efficient infection of murine colonic epithelial cells in vivo. In summary, our study demonstrated that EHEC utilizes NleL E3 ubiquitin ligase activity to modulate Tir-mediated pedestal formation." @default.
- W2096787468 created "2016-06-24" @default.
- W2096787468 creator A5018584334 @default.
- W2096787468 creator A5031816004 @default.
- W2096787468 creator A5032432300 @default.
- W2096787468 creator A5052744787 @default.
- W2096787468 creator A5065030286 @default.
- W2096787468 creator A5072773831 @default.
- W2096787468 creator A5074527727 @default.
- W2096787468 creator A5084039634 @default.
- W2096787468 date "2011-04-26" @default.
- W2096787468 modified "2023-10-01" @default.
- W2096787468 title "The EHEC Type III Effector NleL Is an E3 Ubiquitin Ligase That Modulates Pedestal Formation" @default.
- W2096787468 cites W1532955427 @default.
- W2096787468 cites W1832030339 @default.
- W2096787468 cites W1859520518 @default.
- W2096787468 cites W1895779241 @default.
- W2096787468 cites W1919335671 @default.
- W2096787468 cites W1963967499 @default.
- W2096787468 cites W1966597734 @default.
- W2096787468 cites W1970347595 @default.
- W2096787468 cites W1970910205 @default.
- W2096787468 cites W1975057095 @default.
- W2096787468 cites W1981607669 @default.
- W2096787468 cites W1985396638 @default.
- W2096787468 cites W1988771590 @default.
- W2096787468 cites W1993313171 @default.
- W2096787468 cites W1993562792 @default.
- W2096787468 cites W1995993192 @default.
- W2096787468 cites W1996223704 @default.
- W2096787468 cites W1996766766 @default.
- W2096787468 cites W1997648709 @default.
- W2096787468 cites W1999820067 @default.
- W2096787468 cites W2009146748 @default.
- W2096787468 cites W2013914593 @default.
- W2096787468 cites W2022113253 @default.
- W2096787468 cites W2025728213 @default.
- W2096787468 cites W2029526066 @default.
- W2096787468 cites W2038261860 @default.
- W2096787468 cites W2048428229 @default.
- W2096787468 cites W2048512273 @default.
- W2096787468 cites W2050268205 @default.
- W2096787468 cites W2057132767 @default.
- W2096787468 cites W2060410804 @default.
- W2096787468 cites W2064850779 @default.
- W2096787468 cites W2065231269 @default.
- W2096787468 cites W2065486499 @default.
- W2096787468 cites W2078684325 @default.
- W2096787468 cites W2080554514 @default.
- W2096787468 cites W2084617901 @default.
- W2096787468 cites W2086151987 @default.
- W2096787468 cites W2086966984 @default.
- W2096787468 cites W2087298251 @default.
- W2096787468 cites W2089854636 @default.
- W2096787468 cites W2095744711 @default.
- W2096787468 cites W2099648551 @default.
- W2096787468 cites W2099836442 @default.
- W2096787468 cites W2107201436 @default.
- W2096787468 cites W2112111992 @default.
- W2096787468 cites W2121524482 @default.
- W2096787468 cites W2127135538 @default.
- W2096787468 cites W2130497192 @default.
- W2096787468 cites W2131159435 @default.
- W2096787468 cites W2133258573 @default.
- W2096787468 cites W2135894069 @default.
- W2096787468 cites W2138217241 @default.
- W2096787468 cites W2145397448 @default.
- W2096787468 cites W2146846288 @default.
- W2096787468 cites W2151459483 @default.
- W2096787468 cites W2154824060 @default.
- W2096787468 cites W2157116022 @default.
- W2096787468 cites W2159386862 @default.
- W2096787468 cites W2168641696 @default.
- W2096787468 cites W2171006179 @default.
- W2096787468 cites W2171228833 @default.
- W2096787468 doi "https://doi.org/10.1371/journal.pone.0019331" @default.
- W2096787468 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3082576" @default.
- W2096787468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21541301" @default.
- W2096787468 hasPublicationYear "2011" @default.
- W2096787468 type Work @default.
- W2096787468 sameAs 2096787468 @default.
- W2096787468 citedByCount "42" @default.
- W2096787468 countsByYear W20967874682012 @default.
- W2096787468 countsByYear W20967874682013 @default.
- W2096787468 countsByYear W20967874682014 @default.
- W2096787468 countsByYear W20967874682016 @default.
- W2096787468 countsByYear W20967874682017 @default.
- W2096787468 countsByYear W20967874682018 @default.
- W2096787468 countsByYear W20967874682020 @default.
- W2096787468 countsByYear W20967874682021 @default.
- W2096787468 countsByYear W20967874682022 @default.
- W2096787468 countsByYear W20967874682023 @default.
- W2096787468 crossrefType "journal-article" @default.
- W2096787468 hasAuthorship W2096787468A5018584334 @default.
- W2096787468 hasAuthorship W2096787468A5031816004 @default.
- W2096787468 hasAuthorship W2096787468A5032432300 @default.
- W2096787468 hasAuthorship W2096787468A5052744787 @default.
- W2096787468 hasAuthorship W2096787468A5065030286 @default.