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- W2096809883 abstract "Mutational studies have identified retinoic acid receptor α (RARα) as having an essential role in spermatogenesis. The objective of this study was to determine which cells express RARα within the normal rat testis by conducting in situ analyses for mRNA and protein. Characterization of RARα expression revealed the time and location of the vitamin A requirement during spermatogenesis. In situ hybridization analysis of testis from adult rats showed the highest level of transcripts occurring in round spermatids at stage VIII of the spermatogenic cycle. Analysis in the developing testes revealed that the mRNA level was high from 10 to 15 days of age, both in Sertoli cells and in germ cells, and then declined in 20-day-old rats. Consistent with this, immunohistochemical studies on adult testis demonstrated that the protein was present in the nucleus of the elongating spermatids (stages IX-XI) but not in elongated spermatids. The protein was also expressed in germ cells in the prophase of meiosis and at very low levels in Sertoli cells. These results suggest a role for RARα during meiosis, at the transition from round to elongating spermatids, and in Sertoli cells of developing testis." @default.
- W2096809883 created "2016-06-24" @default.
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- W2096809883 date "1997-02-01" @default.
- W2096809883 modified "2023-09-29" @default.
- W2096809883 title "Retinoic Acid Receptor α Gene Expression in the Rat Testis: Potential Role during the Prophase of Meiosis and in the Transition from Round to Elongating Spermatids" @default.
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- W2096809883 doi "https://doi.org/10.1095/biolreprod56.2.549" @default.
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