FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2096858144> ?p ?o ?g. }

• W2096858144 abstract "HIV-1 transcription is tightly regulated: silent in long-term latency and highly active in acutely-infected cells. Transcription is activated by the viral protein Tat, which recruits the elongation factor P-TEFb by binding the TAR sequence present in nascent HIV-1 RNAs. In this study, we analyzed the dynamic of the TAR:Tat:P-TEFb complex in living cells, by performing FRAP experiments at HIV-1 transcription sites. Our results indicate that a large fraction of Tat present at these sites is recruited by Cyclin T1. We found that in the presence of Tat, Cdk9 remained bound to nascent HIV-1 RNAs for 71s. In contrast, when transcription was activated by PMA/ionomycin, in the absence of Tat, Cdk9 turned-over rapidly and resided on the HIV-1 promoter for only 11s. Thus, the mechanism of trans-activation determines the residency time of P-TEFb at the HIV-1 gene, possibly explaining why Tat is such a potent transcriptional activator. In addition, we observed that Tat occupied HIV-1 transcription sites for 55s, suggesting that the TAR:Tat:P-TEFb complex dissociates from the polymerase following transcription initiation, and undergoes subsequent cycles of association/dissociation." @default.
• W2096858144 created "2016-06-24" @default.
• W2096858144 creator A5000822904 @default.
• W2096858144 creator A5005524911 @default.
• W2096858144 creator A5013896668 @default.
• W2096858144 creator A5030751614 @default.
• W2096858144 creator A5047705603 @default.
• W2096858144 creator A5056221917 @default.
• W2096858144 creator A5084245918 @default.
• W2096858144 date "2007-05-30" @default.
• W2096858144 modified "2023-10-18" @default.
• W2096858144 title "A real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites" @default.
• W2096858144 cites W1971592398 @default.
• W2096858144 cites W1976372760 @default.
• W2096858144 cites W2007496937 @default.
• W2096858144 cites W2019009046 @default.
• W2096858144 cites W2021551755 @default.
• W2096858144 cites W2022043882 @default.
• W2096858144 cites W2046854154 @default.
• W2096858144 cites W2049222491 @default.
• W2096858144 cites W2049874714 @default.
• W2096858144 cites W2057228860 @default.
• W2096858144 cites W2089191944 @default.
• W2096858144 cites W2090040582 @default.
• W2096858144 cites W2120704505 @default.
• W2096858144 cites W2123792331 @default.
• W2096858144 cites W2150217938 @default.
• W2096858144 cites W2171228793 @default.
• W2096858144 doi "https://doi.org/10.1186/1742-4690-4-36" @default.
• W2096858144 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1904240" @default.
• W2096858144 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17537237" @default.
• W2096858144 hasPublicationYear "2007" @default.
• W2096858144 type Work @default.
• W2096858144 sameAs 2096858144 @default.
• W2096858144 citedByCount "51" @default.
• W2096858144 countsByYear W20968581442012 @default.
• W2096858144 countsByYear W20968581442013 @default.
• W2096858144 countsByYear W20968581442014 @default.
• W2096858144 countsByYear W20968581442015 @default.
• W2096858144 countsByYear W20968581442016 @default.
• W2096858144 countsByYear W20968581442017 @default.
• W2096858144 countsByYear W20968581442018 @default.
• W2096858144 countsByYear W20968581442019 @default.
• W2096858144 countsByYear W20968581442020 @default.
• W2096858144 countsByYear W20968581442021 @default.
• W2096858144 countsByYear W20968581442022 @default.
• W2096858144 countsByYear W20968581442023 @default.
• W2096858144 crossrefType "journal-article" @default.
• W2096858144 hasAuthorship W2096858144A5000822904 @default.
• W2096858144 hasAuthorship W2096858144A5005524911 @default.
• W2096858144 hasAuthorship W2096858144A5013896668 @default.
• W2096858144 hasAuthorship W2096858144A5030751614 @default.
• W2096858144 hasAuthorship W2096858144A5047705603 @default.
• W2096858144 hasAuthorship W2096858144A5056221917 @default.
• W2096858144 hasAuthorship W2096858144A5084245918 @default.
• W2096858144 hasBestOaLocation W20968581441 @default.
• W2096858144 hasConcept C101762097 @default.
• W2096858144 hasConcept C104317684 @default.
• W2096858144 hasConcept C138885662 @default.
• W2096858144 hasConcept C150194340 @default.
• W2096858144 hasConcept C153911025 @default.
• W2096858144 hasConcept C179926584 @default.
• W2096858144 hasConcept C185592680 @default.
• W2096858144 hasConcept C2777841055 @default.
• W2096858144 hasConcept C41895202 @default.
• W2096858144 hasConcept C54355233 @default.
• W2096858144 hasConcept C64350747 @default.
• W2096858144 hasConcept C86803240 @default.
• W2096858144 hasConcept C88045685 @default.
• W2096858144 hasConcept C95444343 @default.
• W2096858144 hasConceptScore W2096858144C101762097 @default.
• W2096858144 hasConceptScore W2096858144C104317684 @default.
• W2096858144 hasConceptScore W2096858144C138885662 @default.
• W2096858144 hasConceptScore W2096858144C150194340 @default.
• W2096858144 hasConceptScore W2096858144C153911025 @default.
• W2096858144 hasConceptScore W2096858144C179926584 @default.
• W2096858144 hasConceptScore W2096858144C185592680 @default.
• W2096858144 hasConceptScore W2096858144C2777841055 @default.
• W2096858144 hasConceptScore W2096858144C41895202 @default.
• W2096858144 hasConceptScore W2096858144C54355233 @default.
• W2096858144 hasConceptScore W2096858144C64350747 @default.
• W2096858144 hasConceptScore W2096858144C86803240 @default.
• W2096858144 hasConceptScore W2096858144C88045685 @default.
• W2096858144 hasConceptScore W2096858144C95444343 @default.
• W2096858144 hasIssue "1" @default.
• W2096858144 hasLocation W20968581441 @default.
• W2096858144 hasLocation W20968581442 @default.
• W2096858144 hasLocation W20968581443 @default.
• W2096858144 hasLocation W20968581444 @default.
• W2096858144 hasLocation W20968581445 @default.
• W2096858144 hasOpenAccess W2096858144 @default.
• W2096858144 hasPrimaryLocation W20968581441 @default.
• W2096858144 hasRelatedWork W1971236504 @default.
• W2096858144 hasRelatedWork W1978861852 @default.
• W2096858144 hasRelatedWork W1991771980 @default.
• W2096858144 hasRelatedWork W2096858144 @default.
• W2096858144 hasRelatedWork W2234751501 @default.
• W2096858144 hasRelatedWork W2550274675 @default.
• W2096858144 hasRelatedWork W2587865829 @default.
• W2096858144 hasRelatedWork W2761486126 @default.

• next