FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097069930> ?p ?o ?g. }

• W2097069930 endingPage "484" @default.
• W2097069930 startingPage "475" @default.
• W2097069930 abstract "The proprotein convertases (PCs) participate in the limited proteolysis of integrin α4 subunit at the H592VISKR597 ↓ ST site (where underlined residues indicate positively charged amino acids important for PC-mediated cleavage and ↓ indicates the cleavage site), since this cleavage is inhibited by the serpin α1-PDX (α1-antitrypsin Portland). Co-expression of α4 with each convertase in LoVo (furin-deficient human colon carcinoma) cells revealed that furin and proprotein convertase 5A (PC5A) are the best pro-α4 convertases. In agreement, processing of endogenous pro-α4 in human lymphoblastoid CEM-T4 cells was enhanced greatly in stable transfectants overexpressing either enzyme. In many leucocyte cell lines, the expression of furin closely correlated with the endogenous processing efficacy, suggesting that furin is a candidate pro-α4 convertase. Mutational analysis showed that replacement of P1 Arg597 with alanine (R597A) abrogated cleavage, whereas the P6 mutant H592R is even better processed by the endogenous convertases of Chinese-hamster ovary CHO-K1 cells. In vitro kinetic studies using synthetic peptides confirmed the importance of a positively charged residue at P6 and showed that wild-type α4 processing is performed best by furin and PC5A at acidic and neutral pHs, respectively. Biosynthetic analysis of pro-α4 and its H592R and H592K mutants in the presence or absence of the weak base, NH4Cl, revealed that the P6 histidine residue renders its processing by furin sensitive to cellular pH. This suggests that pro-α4 cleavage occurs preferentially in acidic compartments. In conclusion, although the accepted furin processing motif is Arg-Xaa-(Lys/Arg)-Arg↓, our data further extend it to include a regulatory histidine residue at P6 in precursors that lack a basic residue at P4." @default.
• W2097069930 created "2016-06-24" @default.
• W2097069930 creator A5008431774 @default.
• W2097069930 creator A5012783968 @default.
• W2097069930 creator A5068775377 @default.
• W2097069930 creator A5090995081 @default.
• W2097069930 date "2003-07-15" @default.
• W2097069930 modified "2023-10-18" @default.
• W2097069930 title "Processing of alpha4 integrin by the proprotein convertases: histidine at position P6 regulates cleavage" @default.
• W2097069930 cites W1509556944 @default.
• W2097069930 cites W1524710534 @default.
• W2097069930 cites W1531368146 @default.
• W2097069930 cites W1569326224 @default.
• W2097069930 cites W1576773659 @default.
• W2097069930 cites W1939326986 @default.
• W2097069930 cites W1961749610 @default.
• W2097069930 cites W1968207046 @default.
• W2097069930 cites W1969977810 @default.
• W2097069930 cites W1974043792 @default.
• W2097069930 cites W1980442086 @default.
• W2097069930 cites W1988915040 @default.
• W2097069930 cites W1991576220 @default.
• W2097069930 cites W2009239392 @default.
• W2097069930 cites W2009451560 @default.
• W2097069930 cites W2010215050 @default.
• W2097069930 cites W2016648335 @default.
• W2097069930 cites W2016913070 @default.
• W2097069930 cites W2022352438 @default.
• W2097069930 cites W2032896346 @default.
• W2097069930 cites W2036822252 @default.
• W2097069930 cites W2041427236 @default.
• W2097069930 cites W2046820814 @default.
• W2097069930 cites W2055059015 @default.
• W2097069930 cites W2055362351 @default.
• W2097069930 cites W2062808764 @default.
• W2097069930 cites W2063487760 @default.
• W2097069930 cites W2077614222 @default.
• W2097069930 cites W2082012658 @default.
• W2097069930 cites W2082195162 @default.
• W2097069930 cites W2085875957 @default.
• W2097069930 cites W2093102332 @default.
• W2097069930 cites W2093363945 @default.
• W2097069930 cites W2094469836 @default.
• W2097069930 cites W2096470048 @default.
• W2097069930 cites W2105925178 @default.
• W2097069930 cites W2111443555 @default.
• W2097069930 cites W2120195060 @default.
• W2097069930 cites W2123091875 @default.
• W2097069930 cites W2152002380 @default.
• W2097069930 cites W2162613182 @default.
• W2097069930 cites W2163226372 @default.
• W2097069930 cites W2167853762 @default.
• W2097069930 cites W2169318446 @default.
• W2097069930 cites W2201429489 @default.
• W2097069930 cites W2318767049 @default.
• W2097069930 cites W2339944396 @default.
• W2097069930 cites W2398878900 @default.
• W2097069930 cites W2405730328 @default.
• W2097069930 cites W26067972 @default.
• W2097069930 doi "https://doi.org/10.1042/bj20021630" @default.
• W2097069930 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1223497" @default.
• W2097069930 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12691605" @default.
• W2097069930 hasPublicationYear "2003" @default.
• W2097069930 type Work @default.
• W2097069930 sameAs 2097069930 @default.
• W2097069930 citedByCount "54" @default.
• W2097069930 countsByYear W20970699302012 @default.
• W2097069930 countsByYear W20970699302013 @default.
• W2097069930 countsByYear W20970699302017 @default.
• W2097069930 countsByYear W20970699302019 @default.
• W2097069930 countsByYear W20970699302021 @default.
• W2097069930 countsByYear W20970699302022 @default.
• W2097069930 countsByYear W20970699302023 @default.
• W2097069930 crossrefType "journal-article" @default.
• W2097069930 hasAuthorship W2097069930A5008431774 @default.
• W2097069930 hasAuthorship W2097069930A5012783968 @default.
• W2097069930 hasAuthorship W2097069930A5068775377 @default.
• W2097069930 hasAuthorship W2097069930A5090995081 @default.
• W2097069930 hasBestOaLocation W20970699302 @default.
• W2097069930 hasConcept C104317684 @default.
• W2097069930 hasConcept C143065580 @default.
• W2097069930 hasConcept C151730666 @default.
• W2097069930 hasConcept C153911025 @default.
• W2097069930 hasConcept C170493617 @default.
• W2097069930 hasConcept C175156509 @default.
• W2097069930 hasConcept C175656101 @default.
• W2097069930 hasConcept C181199279 @default.
• W2097069930 hasConcept C185592680 @default.
• W2097069930 hasConcept C2776414213 @default.
• W2097069930 hasConcept C2777265534 @default.
• W2097069930 hasConcept C2778163477 @default.
• W2097069930 hasConcept C2778824825 @default.
• W2097069930 hasConcept C2780072125 @default.
• W2097069930 hasConcept C2781307694 @default.
• W2097069930 hasConcept C2908627167 @default.
• W2097069930 hasConcept C43369102 @default.
• W2097069930 hasConcept C43554185 @default.
• W2097069930 hasConcept C55493867 @default.

• next