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- W209708286 abstract "The chapter focuses on the isolation and culture of the cells of capillary endothelium of the brain, which plays an important role in homeostasis within the central nervous system by limiting the blood to brain passage of some solutes while facilitating exchange of others. This selective permeability barrier—termed the blood–brain barrier—is produced by a combination of specific membrane transport systems, a low rate of pinocytosis, and the presence of continuous intercellular tight junctions, which seal adjacent endothelial cells together. It is difficult to study the properties of brain capillary endothelial cells in vivo because they represent a small fraction of the total cells in the brain. This chapter describes the successful culture of capillary endothelial cells derived from rat brain. Primary cultures retain both endothelial cell markers and some unique features, typical of brain capillary endothelial cells in vivo. This method provides a large number of endothelial cells, which would be useful for studies of brain capillary function. Although brain capillaries initially contain two cell types, the endothelial cells can be separated from the pericytes by gradient centrifugation." @default.
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- W209708286 date "1981-01-01" @default.
- W209708286 modified "2023-09-24" @default.
- W209708286 title "IN VITRO INVESTIGATIONS OF THE BLOOD-BRAIN BARRIER: ISOLATION AND CULTURE OF BRAIN CAPILLARY ENDOTHELIAL CELLS" @default.
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- W209708286 doi "https://doi.org/10.1016/b978-0-08-026819-4.50039-8" @default.
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