FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097105369> ?p ?o ?g. }

• W2097105369 endingPage "993" @default.
• W2097105369 startingPage "981" @default.
• W2097105369 abstract "Cells of the human keratinocyte line HaCaT were shifted to a mesenchymal/myogenic phenotype (DTHMZ cells) by MyoD1 transfection, 5-aza-2' deoxycytidine treatment, and selection for reduced adhesion on plastic. Since this correlated with loss of stratification (inability to form a multilayered tissue), we determined the status of cell-cell and cell-matrix adhesion molecules involved in epidermal morphogenesis. Expression of desmosomal proteins (plakoglobin, desmoglein, desmoplakin) and uvomorulin was no longer detectable at the mRNA and protein level in the DTHMZ cells while both HaCaT cells and malignant variants (transfected with c-Ha-ras oncogene) expressed uvomorulin in vitro and in transplants in vivo, the latter even in invasively growing tumor nodules. Furthermore, HaCaT cells stained positive for the integrin subunits beta 1, alpha 2, alpha 3, and alpha 5, typical for cultured keratinocytes. In contrast, the putative fibronectin receptor alpha 5 beta 1, common also in fibroblasts, was the only integrin showing strong staining in DTHMZ cells. The integrin subunits alpha v and a6, clearly expressed at the mRNA level, weakly stained HaCaT cultures and led to a dotlike fluorescence in DTHMZ cells, possibly representing focal adhesion plaques. The respective integrin status correlated well with the growth behavior on different matrices. While HaCaT cells readily attached and proliferated on collagen (type I), fibronectin-coated, and laminin-coated collagen gels, DTHMZ cells formed monolayers only on fibronectin-coated collagen. This was, however, not sufficient to allow stratification in vivo. Altogether, the status of adhesion molecules in DTHMZ cells more likely reflects that seen in mesenchymal cells as compared to the pattern of keratinocytes displayed by HaCaT cells. Moreover, since the DTHMZ cells were clearly HaCaT descendants, the results support our hypothesis of a trans-differentiation process from an epidermal (HaCaT) to a mesenchymal/myogenic phenotype (DTHMZ)." @default.
• W2097105369 created "2016-06-24" @default.
• W2097105369 creator A5004702190 @default.
• W2097105369 creator A5068941302 @default.
• W2097105369 date "1993-02-15" @default.
• W2097105369 modified "2023-10-18" @default.
• W2097105369 title "Trans-differentiation from epidermal to mesenchymal/myogenic phenotype is associated with a drastic change in cell-cell and cell-matrix adhesion molecules." @default.
• W2097105369 cites W1488492700 @default.
• W2097105369 cites W1529646916 @default.
• W2097105369 cites W1552544560 @default.
• W2097105369 cites W1584106885 @default.
• W2097105369 cites W1594121967 @default.
• W2097105369 cites W1596418087 @default.
• W2097105369 cites W1862598120 @default.
• W2097105369 cites W1957564186 @default.
• W2097105369 cites W1964595762 @default.
• W2097105369 cites W1968870498 @default.
• W2097105369 cites W1973834594 @default.
• W2097105369 cites W1974342547 @default.
• W2097105369 cites W1975818498 @default.
• W2097105369 cites W1977706672 @default.
• W2097105369 cites W1987212762 @default.
• W2097105369 cites W1989074212 @default.
• W2097105369 cites W1990387345 @default.
• W2097105369 cites W1999850734 @default.
• W2097105369 cites W2005165360 @default.
• W2097105369 cites W2008517901 @default.
• W2097105369 cites W2011836510 @default.
• W2097105369 cites W2013381560 @default.
• W2097105369 cites W2020258044 @default.
• W2097105369 cites W2021800397 @default.
• W2097105369 cites W2026930305 @default.
• W2097105369 cites W2029231027 @default.
• W2097105369 cites W2032106871 @default.
• W2097105369 cites W2034713686 @default.
• W2097105369 cites W2036145275 @default.
• W2097105369 cites W2037801769 @default.
• W2097105369 cites W2043418794 @default.
• W2097105369 cites W2047423042 @default.
• W2097105369 cites W2051747697 @default.
• W2097105369 cites W2051811193 @default.
• W2097105369 cites W2060041745 @default.
• W2097105369 cites W2061385217 @default.
• W2097105369 cites W2065051915 @default.
• W2097105369 cites W2065404901 @default.
• W2097105369 cites W2066416662 @default.
• W2097105369 cites W2071790834 @default.
• W2097105369 cites W2084628519 @default.
• W2097105369 cites W2092078806 @default.
• W2097105369 cites W2096133301 @default.
• W2097105369 cites W2105300288 @default.
• W2097105369 cites W2108360007 @default.
• W2097105369 cites W2111072594 @default.
• W2097105369 cites W2120215303 @default.
• W2097105369 cites W2120377208 @default.
• W2097105369 cites W2128940002 @default.
• W2097105369 cites W2131341361 @default.
• W2097105369 cites W2135311582 @default.
• W2097105369 cites W2137949057 @default.
• W2097105369 cites W2138523931 @default.
• W2097105369 cites W2141966220 @default.
• W2097105369 cites W2143464311 @default.
• W2097105369 cites W2147816965 @default.
• W2097105369 cites W2148175545 @default.
• W2097105369 cites W2161140556 @default.
• W2097105369 cites W2465967309 @default.
• W2097105369 cites W2576734941 @default.
• W2097105369 cites W35262646 @default.
• W2097105369 doi "https://doi.org/10.1083/jcb.120.4.981" @default.
• W2097105369 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2200069" @default.
• W2097105369 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8381787" @default.
• W2097105369 hasPublicationYear "1993" @default.
• W2097105369 type Work @default.
• W2097105369 sameAs 2097105369 @default.
• W2097105369 citedByCount "31" @default.
• W2097105369 countsByYear W20971053692018 @default.
• W2097105369 countsByYear W20971053692020 @default.
• W2097105369 crossrefType "journal-article" @default.
• W2097105369 hasAuthorship W2097105369A5004702190 @default.
• W2097105369 hasAuthorship W2097105369A5068941302 @default.
• W2097105369 hasBestOaLocation W20971053691 @default.
• W2097105369 hasConcept C118995209 @default.
• W2097105369 hasConcept C1491633281 @default.
• W2097105369 hasConcept C153911025 @default.
• W2097105369 hasConcept C16224149 @default.
• W2097105369 hasConcept C189165786 @default.
• W2097105369 hasConcept C195687474 @default.
• W2097105369 hasConcept C198826908 @default.
• W2097105369 hasConcept C2779814568 @default.
• W2097105369 hasConcept C54355233 @default.
• W2097105369 hasConcept C55493867 @default.
• W2097105369 hasConcept C81885089 @default.
• W2097105369 hasConcept C85789140 @default.
• W2097105369 hasConcept C86492073 @default.
• W2097105369 hasConcept C86803240 @default.
• W2097105369 hasConcept C95444343 @default.
• W2097105369 hasConceptScore W2097105369C118995209 @default.
• W2097105369 hasConceptScore W2097105369C1491633281 @default.

• next