FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097173617> ?p ?o ?g. }

• W2097173617 endingPage "5413" @default.
• W2097173617 startingPage "5404" @default.
• W2097173617 abstract "Selectins are a family of lectins, that mediate tethering and rolling of leukocytes on endothelium in vascular shear flow. Mild periodate oxidation of the L-selectin ligand CD34, or L-selectin ligands on leukocytes, enhanced resistance to detachment in shear and decreased rolling velocity equivalent to an 8-fold increase in ligand density, yet had little effect on the rate of tethering. Enhanced interactions were also seen with mildly oxidized sialyl Lewisa and sialyl Lewisx glycolipids. Enhancement was completely reversed by borohydride reduction, yielding a strength of interaction equivalent to that with the native ligands. No effect on the strength of P-selectin and E-selectin interactions was seen after mild oxidation of their ligands. Completeness of modification of sialic acid by mild periodate was verified with monoclonal antibody to sialyl Lewisx-related structures and resistance to neuraminidase. The addition of cyanoborohydride to leukocytes rolling through L-selectin on mildly oxidized but not native CD34 caused arrest of rolling cells and formation of EDTA-resistant bonds to the substrate, suggesting that a Schiff base was reduced. Cyanoborohydride reduction of mildly oxidized cells rolling on P-selectin and E-selectin also caused arrest and formation of EDTA-resistant bonds but with slower kinetics. These data suggest that interactions with a sialic acid aldehyde group on mildly oxidized ligands that include interconversion to a Schiff base can occur with three selectins yet only stabilize binding through the selectin with the fastest koff, L-selectin. Selectins are a family of lectins, that mediate tethering and rolling of leukocytes on endothelium in vascular shear flow. Mild periodate oxidation of the L-selectin ligand CD34, or L-selectin ligands on leukocytes, enhanced resistance to detachment in shear and decreased rolling velocity equivalent to an 8-fold increase in ligand density, yet had little effect on the rate of tethering. Enhanced interactions were also seen with mildly oxidized sialyl Lewisa and sialyl Lewisx glycolipids. Enhancement was completely reversed by borohydride reduction, yielding a strength of interaction equivalent to that with the native ligands. No effect on the strength of P-selectin and E-selectin interactions was seen after mild oxidation of their ligands. Completeness of modification of sialic acid by mild periodate was verified with monoclonal antibody to sialyl Lewisx-related structures and resistance to neuraminidase. The addition of cyanoborohydride to leukocytes rolling through L-selectin on mildly oxidized but not native CD34 caused arrest of rolling cells and formation of EDTA-resistant bonds to the substrate, suggesting that a Schiff base was reduced. Cyanoborohydride reduction of mildly oxidized cells rolling on P-selectin and E-selectin also caused arrest and formation of EDTA-resistant bonds but with slower kinetics. These data suggest that interactions with a sialic acid aldehyde group on mildly oxidized ligands that include interconversion to a Schiff base can occur with three selectins yet only stabilize binding through the selectin with the fastest koff, L-selectin." @default.
• W2097173617 created "2016-06-24" @default.
• W2097173617 creator A5003615852 @default.
• W2097173617 creator A5035698314 @default.
• W2097173617 date "1996-03-01" @default.
• W2097173617 modified "2023-10-18" @default.
• W2097173617 title "A Schiff Base with Mildly Oxidized Carbohydrate Ligands Stabilizes L-selectin and not P-selectin or E-selectin Rolling Adhesions in Shear Flow" @default.
• W2097173617 cites W1487121574 @default.
• W2097173617 cites W1544936755 @default.
• W2097173617 cites W1566087596 @default.
• W2097173617 cites W1641373107 @default.
• W2097173617 cites W1751281485 @default.
• W2097173617 cites W1898209536 @default.
• W2097173617 cites W1984335380 @default.
• W2097173617 cites W1989978944 @default.
• W2097173617 cites W1994521388 @default.
• W2097173617 cites W2006018450 @default.
• W2097173617 cites W2011134736 @default.
• W2097173617 cites W2016573683 @default.
• W2097173617 cites W2024597511 @default.
• W2097173617 cites W2031900413 @default.
• W2097173617 cites W2032371793 @default.
• W2097173617 cites W2034185665 @default.
• W2097173617 cites W2037641196 @default.
• W2097173617 cites W2041111200 @default.
• W2097173617 cites W2047748391 @default.
• W2097173617 cites W2047916715 @default.
• W2097173617 cites W2048528503 @default.
• W2097173617 cites W2050583710 @default.
• W2097173617 cites W2055596401 @default.
• W2097173617 cites W2058457179 @default.
• W2097173617 cites W2075939418 @default.
• W2097173617 cites W2080780289 @default.
• W2097173617 cites W2081030170 @default.
• W2097173617 cites W2106473403 @default.
• W2097173617 cites W2116198597 @default.
• W2097173617 cites W2121783932 @default.
• W2097173617 cites W2138819257 @default.
• W2097173617 cites W2148997371 @default.
• W2097173617 cites W2149331843 @default.
• W2097173617 cites W2157681283 @default.
• W2097173617 cites W2159588837 @default.
• W2097173617 cites W2166207391 @default.
• W2097173617 cites W2166281746 @default.
• W2097173617 cites W2576742884 @default.
• W2097173617 cites W30942276 @default.
• W2097173617 cites W4240558947 @default.
• W2097173617 cites W4246991801 @default.
• W2097173617 doi "https://doi.org/10.1074/jbc.271.10.5404" @default.
• W2097173617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8621395" @default.
• W2097173617 hasPublicationYear "1996" @default.
• W2097173617 type Work @default.
• W2097173617 sameAs 2097173617 @default.
• W2097173617 citedByCount "17" @default.
• W2097173617 crossrefType "journal-article" @default.
• W2097173617 hasAuthorship W2097173617A5003615852 @default.
• W2097173617 hasAuthorship W2097173617A5035698314 @default.
• W2097173617 hasBestOaLocation W20971736171 @default.
• W2097173617 hasConcept C116569031 @default.
• W2097173617 hasConcept C155619193 @default.
• W2097173617 hasConcept C170493617 @default.
• W2097173617 hasConcept C178790620 @default.
• W2097173617 hasConcept C185592680 @default.
• W2097173617 hasConcept C203014093 @default.
• W2097173617 hasConcept C2776789926 @default.
• W2097173617 hasConcept C2780115458 @default.
• W2097173617 hasConcept C3018697912 @default.
• W2097173617 hasConcept C55493867 @default.
• W2097173617 hasConcept C56701166 @default.
• W2097173617 hasConcept C71240020 @default.
• W2097173617 hasConcept C84416704 @default.
• W2097173617 hasConcept C86803240 @default.
• W2097173617 hasConcept C89560881 @default.
• W2097173617 hasConceptScore W2097173617C116569031 @default.
• W2097173617 hasConceptScore W2097173617C155619193 @default.
• W2097173617 hasConceptScore W2097173617C170493617 @default.
• W2097173617 hasConceptScore W2097173617C178790620 @default.
• W2097173617 hasConceptScore W2097173617C185592680 @default.
• W2097173617 hasConceptScore W2097173617C203014093 @default.
• W2097173617 hasConceptScore W2097173617C2776789926 @default.
• W2097173617 hasConceptScore W2097173617C2780115458 @default.
• W2097173617 hasConceptScore W2097173617C3018697912 @default.
• W2097173617 hasConceptScore W2097173617C55493867 @default.
• W2097173617 hasConceptScore W2097173617C56701166 @default.
• W2097173617 hasConceptScore W2097173617C71240020 @default.
• W2097173617 hasConceptScore W2097173617C84416704 @default.
• W2097173617 hasConceptScore W2097173617C86803240 @default.
• W2097173617 hasConceptScore W2097173617C89560881 @default.
• W2097173617 hasIssue "10" @default.
• W2097173617 hasLocation W20971736171 @default.
• W2097173617 hasOpenAccess W2097173617 @default.
• W2097173617 hasPrimaryLocation W20971736171 @default.
• W2097173617 hasRelatedWork W1528569576 @default.
• W2097173617 hasRelatedWork W1987525409 @default.
• W2097173617 hasRelatedWork W2064095221 @default.
• W2097173617 hasRelatedWork W2144222210 @default.
• W2097173617 hasRelatedWork W2146061875 @default.
• W2097173617 hasRelatedWork W2186326313 @default.

• next