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• W2097200176 abstract "ABSTRACT A new insertion element, IS 1549 , was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS 6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. Six of the kanamycin-resistant isolates had a previously unidentified insertion element upstream of the ORF of the aph gene. The 1,634-bp sequence contained a single ORF of 504 amino acids with 85% G+C content in the third codon position. The putative protein sequence showed a distant relationship to the transposase of IS 231 , which is a member of the IS 4 family of insertion elements. IS 1549 contains 11-bp terminal inverted repeats and is characterized by the formation of unusually long and variable-length (71- to 246-bp) direct repeats of the target DNA during transposition. Southern blot analysis revealed that five copies of IS 1549 are present in LR222, but not all M. smegmatis strains carry this element. Only strains with a 65-kDa antigen gene with a PCR-restriction fragment length polymorphism type identical to that of M. smegmatis 607 contain IS 1549 . None of 13 other species of Mycobacterium tested by PCR with two sets of primers specific for IS 1549 were positive for the expected amplified product." @default.
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• W2097200176 date "1998-03-01" @default.
• W2097200176 modified "2023-10-18" @default.
• W2097200176 title "IS 1549 from Mycobacterium smegmatis Forms Long Direct Repeats upon Insertion" @default.
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• W2097200176 doi "https://doi.org/10.1128/jb.180.5.1037-1043.1998" @default.
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