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• W2097200312 abstract "Human immunodeficiency virus type 1 (HIV-1) infects both activated CD4+ T cells and macrophages. We tested if liquid chromatography–tandem mass spectrometry (LC–MS/MS) technology can monitor metabolic alterations induced by HIV-1 in the infected cells. Here we monitored glucose uptake and conducted LC–MS/MS-based metabolomic analysis in HIV-1 infected primary human CD4+ T cells and a macrophage model system: differentiated U1 (HIV-1 producing) and differentiated U937 (control) cells. HIV-1 infected CD4+ T cells have higher glucose uptake and increases in several metabolite pool sizes, whereas HIV-1 producing macrophages had substantial reductions in glucose uptake and steady state glycolytic intermediates. This data suggests that the two HIV-1 target cell types exhibit very different metabolic outcomes during viral production. This study also validates the LC–MS/MS technology as an effective metabolomic approach to monitor various metabolic alterations made by HIV-1 infection." @default.
• W2097200312 created "2016-06-24" @default.
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• W2097200312 creator A5016571607 @default.
• W2097200312 creator A5085865289 @default.
• W2097200312 date "2011-07-01" @default.
• W2097200312 modified "2023-10-17" @default.
• W2097200312 title "Metabolite profiles of human immunodeficiency virus infected CD4+ T cells and macrophages using LC–MS/MS analysis" @default.
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• W2097200312 doi "https://doi.org/10.1016/j.virol.2011.04.007" @default.
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