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- W2097224367 abstract "We have developed an experimental procedure to analyze the spatial proximity of nuclear matrix-bound DNA fragments. This protocol, referred to as Matrix 3C (M3C), includes a high salt extraction of nuclei, the removal of distal parts of unfolded DNA loops using restriction enzyme treatment, ligation of the nuclear matrix-bound DNA fragments and a subsequent analysis of ligation frequencies. Using the M3C procedure, we have demonstrated that CpG islands of at least three housekeeping genes that surround the chicken α-globin gene domain are assembled into a complex (presumably, a transcription factory) that is stabilized by the nuclear matrix in both erythroid and non-erythroid cells. In erythroid cells, the regulatory elements of the α-globin genes are attracted to this complex to form a new assembly: an active chromatin hub that is linked to the pre-existing transcription factory. The erythroid-specific part of the assembly is removed by high salt extraction. Based on these observations, we propose that mixed transcription factories that mediate the transcription of both housekeeping and tissue-specific genes are composed of a permanent compartment containing integrated into the nuclear matrix promoters of housekeeping genes and a 'guest' compartment where promoters and regulatory elements of tissue-specific genes can be temporarily recruited." @default.
- W2097224367 created "2016-06-24" @default.
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- W2097224367 date "2010-08-12" @default.
- W2097224367 modified "2023-10-09" @default.
- W2097224367 title "Mapping of the nuclear matrix-bound chromatin hubs by a new M3C experimental procedure" @default.
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- W2097224367 doi "https://doi.org/10.1093/nar/gkq712" @default.
- W2097224367 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3001081" @default.
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