Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097259856> ?p ?o ?g. }
- W2097259856 endingPage "1264" @default.
- W2097259856 startingPage "1252" @default.
- W2097259856 abstract "In black cherry (Prunus serotina Ehrh.) seed homogenates, (R)-amygdalin is degraded to HCN, benzaldehyde, and glucose by the sequential action of amygdalin hydrolase (AH), prunasin hydrolase (PH), and mandelonitrile lyase. Leaves are also highly cyanogenic because they possess (R)-prunasin, PH, and mandelonitrile lyase. Taking both enzymological and molecular approaches, we demonstrate here that black cherry PH is encoded by a putative multigene family of at least five members. Their respective cDNAs (designated Ph1, Ph2, Ph3, Ph4, and Ph5) predict isoforms that share 49% to 92% amino acid identity with members of glycoside hydrolase family 1, including their catalytic asparagine-glutamate-proline and isoleucine-threonine-glutamate-asparagine-glycine motifs. Furthermore, consistent with the vacuolar/protein body location and glycoprotein character of these hydrolases, their open reading frames predict N-terminal signal sequences and multiple potential N-glycosylation sites. Genomic sequences corresponding to the open reading frames of these PHs and of the previously isolated AH1 isoform are interrupted at identical positions by 12 introns. Earlier studies established that native AH and PH display strict specificities toward their respective glucosidic substrates. Such behavior was also shown by recombinant AH1, PH2, and PH4 proteins after expression in Pichia pastoris. Three amino acid moieties that may play a role in conferring such aglycone specificities were predicted by structural modeling and comparative sequence analysis and tested by introducing single and multiple mutations into isoform AH1 by site-directed mutagenesis. The double mutant AH ID (Y200I and G394D) hydrolyzed prunasin at approximately 150% of the rate of amygdalin hydrolysis, whereas the other mutations failed to engender PH activity." @default.
- W2097259856 created "2016-06-24" @default.
- W2097259856 creator A5006710285 @default.
- W2097259856 creator A5013378515 @default.
- W2097259856 creator A5026752416 @default.
- W2097259856 creator A5038896865 @default.
- W2097259856 date "2002-07-01" @default.
- W2097259856 modified "2023-09-23" @default.
- W2097259856 title "Investigation of the Microheterogeneity and Aglycone Specificity-Conferring Residues of Black Cherry Prunasin Hydrolases" @default.
- W2097259856 cites W104974149 @default.
- W2097259856 cites W1485045313 @default.
- W2097259856 cites W1830745998 @default.
- W2097259856 cites W18986866 @default.
- W2097259856 cites W1964470639 @default.
- W2097259856 cites W1967255766 @default.
- W2097259856 cites W1972233746 @default.
- W2097259856 cites W1980432226 @default.
- W2097259856 cites W1983515719 @default.
- W2097259856 cites W1983518953 @default.
- W2097259856 cites W1994981235 @default.
- W2097259856 cites W2000614854 @default.
- W2097259856 cites W2003759854 @default.
- W2097259856 cites W2009052919 @default.
- W2097259856 cites W2009310436 @default.
- W2097259856 cites W2013029018 @default.
- W2097259856 cites W2014382116 @default.
- W2097259856 cites W2018289835 @default.
- W2097259856 cites W2019896547 @default.
- W2097259856 cites W2019992430 @default.
- W2097259856 cites W2024127885 @default.
- W2097259856 cites W2024672161 @default.
- W2097259856 cites W2046796922 @default.
- W2097259856 cites W2050135937 @default.
- W2097259856 cites W2055043387 @default.
- W2097259856 cites W2056889633 @default.
- W2097259856 cites W2058633388 @default.
- W2097259856 cites W2062566279 @default.
- W2097259856 cites W2063554961 @default.
- W2097259856 cites W2065170448 @default.
- W2097259856 cites W207446077 @default.
- W2097259856 cites W2079847277 @default.
- W2097259856 cites W2083506978 @default.
- W2097259856 cites W2093162077 @default.
- W2097259856 cites W2097706568 @default.
- W2097259856 cites W2100837269 @default.
- W2097259856 cites W2105661606 @default.
- W2097259856 cites W2106121486 @default.
- W2097259856 cites W2110471634 @default.
- W2097259856 cites W2113964973 @default.
- W2097259856 cites W2126217473 @default.
- W2097259856 cites W2128369415 @default.
- W2097259856 cites W2136724769 @default.
- W2097259856 cites W2137670334 @default.
- W2097259856 cites W2138270253 @default.
- W2097259856 cites W2156030952 @default.
- W2097259856 cites W2314036089 @default.
- W2097259856 cites W2443645127 @default.
- W2097259856 cites W2476872808 @default.
- W2097259856 cites W2492283735 @default.
- W2097259856 cites W2494221614 @default.
- W2097259856 cites W2612985863 @default.
- W2097259856 cites W296560601 @default.
- W2097259856 cites W2992741155 @default.
- W2097259856 cites W3148493843 @default.
- W2097259856 cites W4206781359 @default.
- W2097259856 cites W4247377269 @default.
- W2097259856 cites W4294307372 @default.
- W2097259856 cites W610783292 @default.
- W2097259856 doi "https://doi.org/10.1104/pp.010863" @default.
- W2097259856 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/166519" @default.
- W2097259856 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12114579" @default.
- W2097259856 hasPublicationYear "2002" @default.
- W2097259856 type Work @default.
- W2097259856 sameAs 2097259856 @default.
- W2097259856 citedByCount "51" @default.
- W2097259856 countsByYear W20972598562012 @default.
- W2097259856 countsByYear W20972598562013 @default.
- W2097259856 countsByYear W20972598562014 @default.
- W2097259856 countsByYear W20972598562015 @default.
- W2097259856 countsByYear W20972598562017 @default.
- W2097259856 countsByYear W20972598562018 @default.
- W2097259856 countsByYear W20972598562021 @default.
- W2097259856 countsByYear W20972598562023 @default.
- W2097259856 crossrefType "journal-article" @default.
- W2097259856 hasAuthorship W2097259856A5006710285 @default.
- W2097259856 hasAuthorship W2097259856A5013378515 @default.
- W2097259856 hasAuthorship W2097259856A5026752416 @default.
- W2097259856 hasAuthorship W2097259856A5038896865 @default.
- W2097259856 hasBestOaLocation W20972598561 @default.
- W2097259856 hasConcept C104317684 @default.
- W2097259856 hasConcept C142724271 @default.
- W2097259856 hasConcept C181199279 @default.
- W2097259856 hasConcept C185592680 @default.
- W2097259856 hasConcept C204787440 @default.
- W2097259856 hasConcept C2775895851 @default.
- W2097259856 hasConcept C2780120296 @default.
- W2097259856 hasConcept C2781274105 @default.
- W2097259856 hasConcept C2781372317 @default.