FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097272935> ?p ?o ?g. }

• W2097272935 abstract "The enzyme-linked immunosorbent assay (ELISA) has been used for diagnosing medical and plant pathologies. In addition, it is used for quality-control evaluations in various industries. The ELISA is the simplest method for obtaining excellent results; however, it is time consuming because the immunoreagents interact only on the contact surfaces. Antibody-labeled magnetic particles can be dispersed in a solution to yield a pseudohomogeneous reaction with antigens which improved the efficiency of immunoreaction, and can be easily separated from the unreactive substances by applying a magnetic force. We used a homemade magnetic microplate, functional magnetic particles (MPs) and enzyme-labeled secondary antibody to perform the sandwich ELISA successfully.Using antibody-labeled MPs enabled reducing the analysis time to one-third of that required in using a conventional ELISA. The secondary antibody conjugated with horseradish peroxidase (HRP) was affinity-bound to the analyte (IgG in this study). The calibration curve was established according to the measured absorbance of the 3, 3', 5, 5'-tetramethybezidine-HRP reaction products versus the concentrations of standard IgG. The linear range of IgG detection was 114 ng/mL-3.5 ng/mL. The limit of detection (LOD) of IgG was 3.4 ng/mL. The recovery and coefficient of variation were 100% (±7%) and 116% (±4%) for the spiked concentrations of 56.8 ng/mL and 14.2 ng/mL, respectively.Pseudohomogeneous reactions can be performed using functional MPs and a magnetic microplate. Using antibody-labeled MPs, the analysis time can be reduced to one-third of that required in using a conventional ELISA. The substrate-enzyme reaction products can be easily transferred to another microplate, and their absorbance can be measured without interference by light scattering caused by magnetic microbeads. This method demonstrates great potential for detecting other biomarkers and in biochemical applications. Graphical AbstractA magnetic ELISA with convenient magnetic microplate." @default.
• W2097272935 created "2016-06-24" @default.
• W2097272935 creator A5006157599 @default.
• W2097272935 creator A5040312742 @default.
• W2097272935 creator A5048888476 @default.
• W2097272935 creator A5062616822 @default.
• W2097272935 creator A5075967913 @default.
• W2097272935 creator A5087174450 @default.
• W2097272935 date "2015-02-22" @default.
• W2097272935 modified "2023-09-26" @default.
• W2097272935 title "Detection of rabbit IgG by using functional magnetic particles and an enzyme-conjugated antibody with a homemade magnetic microplate" @default.
• W2097272935 cites W1967357257 @default.
• W2097272935 cites W1975778282 @default.
• W2097272935 cites W1997181633 @default.
• W2097272935 cites W1997522247 @default.
• W2097272935 cites W1998074949 @default.
• W2097272935 cites W1999104248 @default.
• W2097272935 cites W2005200418 @default.
• W2097272935 cites W2007714156 @default.
• W2097272935 cites W2032754370 @default.
• W2097272935 cites W2057387234 @default.
• W2097272935 cites W2059834357 @default.
• W2097272935 cites W2071124112 @default.
• W2097272935 cites W2082459411 @default.
• W2097272935 cites W2090106308 @default.
• W2097272935 cites W2094106919 @default.
• W2097272935 cites W2131009328 @default.
• W2097272935 cites W2155571084 @default.
• W2097272935 cites W2317644617 @default.
• W2097272935 cites W2318269276 @default.
• W2097272935 cites W2441096350 @default.
• W2097272935 cites W4294402008 @default.
• W2097272935 doi "https://doi.org/10.1186/s13065-015-0088-1" @default.
• W2097272935 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4350001" @default.
• W2097272935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25745512" @default.
• W2097272935 hasPublicationYear "2015" @default.
• W2097272935 type Work @default.
• W2097272935 sameAs 2097272935 @default.
• W2097272935 citedByCount "9" @default.
• W2097272935 countsByYear W20972729352015 @default.
• W2097272935 countsByYear W20972729352016 @default.
• W2097272935 countsByYear W20972729352018 @default.
• W2097272935 countsByYear W20972729352020 @default.
• W2097272935 countsByYear W20972729352022 @default.
• W2097272935 countsByYear W20972729352023 @default.
• W2097272935 crossrefType "journal-article" @default.
• W2097272935 hasAuthorship W2097272935A5006157599 @default.
• W2097272935 hasAuthorship W2097272935A5040312742 @default.
• W2097272935 hasAuthorship W2097272935A5048888476 @default.
• W2097272935 hasAuthorship W2097272935A5062616822 @default.
• W2097272935 hasAuthorship W2097272935A5075967913 @default.
• W2097272935 hasAuthorship W2097272935A5087174450 @default.
• W2097272935 hasBestOaLocation W20972729351 @default.
• W2097272935 hasConcept C10390740 @default.
• W2097272935 hasConcept C112613896 @default.
• W2097272935 hasConcept C119128265 @default.
• W2097272935 hasConcept C134121241 @default.
• W2097272935 hasConcept C155672457 @default.
• W2097272935 hasConcept C159654299 @default.
• W2097272935 hasConcept C171250308 @default.
• W2097272935 hasConcept C178790620 @default.
• W2097272935 hasConcept C181199279 @default.
• W2097272935 hasConcept C185592680 @default.
• W2097272935 hasConcept C191897082 @default.
• W2097272935 hasConcept C192562407 @default.
• W2097272935 hasConcept C203014093 @default.
• W2097272935 hasConcept C21269302 @default.
• W2097272935 hasConcept C23531484 @default.
• W2097272935 hasConcept C2777676563 @default.
• W2097272935 hasConcept C43617362 @default.
• W2097272935 hasConcept C521977710 @default.
• W2097272935 hasConcept C55493867 @default.
• W2097272935 hasConcept C70784841 @default.
• W2097272935 hasConcept C86803240 @default.
• W2097272935 hasConcept C87023908 @default.
• W2097272935 hasConcept C98015330 @default.
• W2097272935 hasConceptScore W2097272935C10390740 @default.
• W2097272935 hasConceptScore W2097272935C112613896 @default.
• W2097272935 hasConceptScore W2097272935C119128265 @default.
• W2097272935 hasConceptScore W2097272935C134121241 @default.
• W2097272935 hasConceptScore W2097272935C155672457 @default.
• W2097272935 hasConceptScore W2097272935C159654299 @default.
• W2097272935 hasConceptScore W2097272935C171250308 @default.
• W2097272935 hasConceptScore W2097272935C178790620 @default.
• W2097272935 hasConceptScore W2097272935C181199279 @default.
• W2097272935 hasConceptScore W2097272935C185592680 @default.
• W2097272935 hasConceptScore W2097272935C191897082 @default.
• W2097272935 hasConceptScore W2097272935C192562407 @default.
• W2097272935 hasConceptScore W2097272935C203014093 @default.
• W2097272935 hasConceptScore W2097272935C21269302 @default.
• W2097272935 hasConceptScore W2097272935C23531484 @default.
• W2097272935 hasConceptScore W2097272935C2777676563 @default.
• W2097272935 hasConceptScore W2097272935C43617362 @default.
• W2097272935 hasConceptScore W2097272935C521977710 @default.
• W2097272935 hasConceptScore W2097272935C55493867 @default.
• W2097272935 hasConceptScore W2097272935C70784841 @default.
• W2097272935 hasConceptScore W2097272935C86803240 @default.
• W2097272935 hasConceptScore W2097272935C87023908 @default.
• W2097272935 hasConceptScore W2097272935C98015330 @default.
• W2097272935 hasIssue "1" @default.

• next