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- W2097334525 abstract "ObjectiveTo determine the mitochondrial DNA and nuclear DNA fragmentation of sperm populations separated by using discontinuous density gradient.DesignAnalysis of mitochondrial and nuclear DNA status of sperm from high and low density layers.SettingRegional fertility center.Patient(s)Twenty-eight men who presented for an initial infertility investigation.Main outcome measure(s)Semen was prepared by using discontinuous density gradient (90.0%:45.0%) and subjected to a modified long polymerase chain reaction to assess mitochondrial DNA deletions and to a modified single-cell alkaline gel electrophoresis assay to determine nuclear DNA fragmentation.Result(s)The high-density fraction displayed significantly more wild-type mitochondrial DNA (75% of samples) than did the low-density fraction (25% of samples). In the high-density fraction, the incidence of single deletions was higher than that of double or multiple deletions, and the deletions were predominantly small. A strong correlation was observed between nuclear DNA fragmentation and the number and size of mitochondrial DNA deletions.Conclusion(s)Density centrifugation isolates a population of sperm with high-quality mitochondrial DNA and nuclear DNA." @default.
- W2097334525 created "2016-06-24" @default.
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- W2097334525 date "2003-03-01" @default.
- W2097334525 modified "2023-10-01" @default.
- W2097334525 title "Differences in mitochondrial and nuclear DNA status of high-density and low-density sperm fractions after density centrifugation preparation" @default.
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- W2097334525 doi "https://doi.org/10.1016/s0015-0282(02)04827-6" @default.
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