Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097377025> ?p ?o ?g. }
- W2097377025 endingPage "336" @default.
- W2097377025 startingPage "331" @default.
- W2097377025 abstract "Serum titers of antibody to Epstein-Barr virus (EBV) viral capsid antigen (VCA) have been positively correlated with malignancies of lymphoid proliferation, such as Burkitt's lymphoma and Hodgkin's lymphoma. We have constructed a phage display combinatorial antibody Fab library from a patient with marginal zone B cell lymphoma associated with Sjögren's syndrome and carrying high serum anti-EBV-VCA IgG titer. Fab fragments were selected by panning against EBV-VCA protein coated onto ELISA plates, and selected Fab clones were characterized by ELISA, western blotting (WB), indirect immunofluorescence assay and immunohistochemistry. We have established two Fab clones, Fab-aVCA1 and Fab-aVCA21, which specifically recognize EBV-VCA by ELISA and WB. Inhibition ELISA competition showed that both clones could significantly reduce the binding of specific anti-EBV-VCA mAb to its relevant proteins. Furthermore, these two Fab clones could localize VCA protein in the EBV-positive P3HR1 and Daudi cell lines, as well as in tissue samples from patients with EBV-infected lymphoid malignancies. These results indicate that our two Fab clones are novel human mAbs specific for EBV-VCA protein and may have potential benefits for development of novel diagnostic and therapeutic approaches in EBV-related lymphoid malignancies." @default.
- W2097377025 created "2016-06-24" @default.
- W2097377025 creator A5014411218 @default.
- W2097377025 creator A5021894128 @default.
- W2097377025 creator A5022142826 @default.
- W2097377025 creator A5024031341 @default.
- W2097377025 creator A5024487337 @default.
- W2097377025 creator A5028431857 @default.
- W2097377025 creator A5046840576 @default.
- W2097377025 creator A5059957465 @default.
- W2097377025 creator A5060679925 @default.
- W2097377025 creator A5061841627 @default.
- W2097377025 creator A5069916638 @default.
- W2097377025 creator A5073388784 @default.
- W2097377025 creator A5074030259 @default.
- W2097377025 creator A5077950778 @default.
- W2097377025 creator A5078409741 @default.
- W2097377025 creator A5091657418 @default.
- W2097377025 date "2007-02-07" @default.
- W2097377025 modified "2023-09-27" @default.
- W2097377025 title "Cloning and expression of two human recombinant monoclonal Fab fragments specific for EBV viral capsid antigen" @default.
- W2097377025 cites W1544117098 @default.
- W2097377025 cites W1630076302 @default.
- W2097377025 cites W1657231595 @default.
- W2097377025 cites W1866836884 @default.
- W2097377025 cites W1884230823 @default.
- W2097377025 cites W1890149945 @default.
- W2097377025 cites W1971456299 @default.
- W2097377025 cites W1973214468 @default.
- W2097377025 cites W1975562645 @default.
- W2097377025 cites W1987058337 @default.
- W2097377025 cites W1992594661 @default.
- W2097377025 cites W1999193464 @default.
- W2097377025 cites W2027522813 @default.
- W2097377025 cites W2029689855 @default.
- W2097377025 cites W2031343644 @default.
- W2097377025 cites W2053892008 @default.
- W2097377025 cites W2057251299 @default.
- W2097377025 cites W2062130049 @default.
- W2097377025 cites W2080385928 @default.
- W2097377025 cites W2089709237 @default.
- W2097377025 cites W2090801666 @default.
- W2097377025 cites W2095993904 @default.
- W2097377025 cites W2102670802 @default.
- W2097377025 cites W2103480414 @default.
- W2097377025 cites W2108311858 @default.
- W2097377025 cites W2118745945 @default.
- W2097377025 cites W2129662814 @default.
- W2097377025 cites W2138911228 @default.
- W2097377025 cites W2141615510 @default.
- W2097377025 cites W2153013045 @default.
- W2097377025 cites W2156685281 @default.
- W2097377025 cites W2158178504 @default.
- W2097377025 cites W2163595349 @default.
- W2097377025 cites W2067635433 @default.
- W2097377025 doi "https://doi.org/10.1093/intimm/dxl150" @default.
- W2097377025 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17272286" @default.
- W2097377025 hasPublicationYear "2007" @default.
- W2097377025 type Work @default.
- W2097377025 sameAs 2097377025 @default.
- W2097377025 citedByCount "7" @default.
- W2097377025 countsByYear W20973770252012 @default.
- W2097377025 countsByYear W20973770252015 @default.
- W2097377025 countsByYear W20973770252017 @default.
- W2097377025 crossrefType "journal-article" @default.
- W2097377025 hasAuthorship W2097377025A5014411218 @default.
- W2097377025 hasAuthorship W2097377025A5021894128 @default.
- W2097377025 hasAuthorship W2097377025A5022142826 @default.
- W2097377025 hasAuthorship W2097377025A5024031341 @default.
- W2097377025 hasAuthorship W2097377025A5024487337 @default.
- W2097377025 hasAuthorship W2097377025A5028431857 @default.
- W2097377025 hasAuthorship W2097377025A5046840576 @default.
- W2097377025 hasAuthorship W2097377025A5059957465 @default.
- W2097377025 hasAuthorship W2097377025A5060679925 @default.
- W2097377025 hasAuthorship W2097377025A5061841627 @default.
- W2097377025 hasAuthorship W2097377025A5069916638 @default.
- W2097377025 hasAuthorship W2097377025A5073388784 @default.
- W2097377025 hasAuthorship W2097377025A5074030259 @default.
- W2097377025 hasAuthorship W2097377025A5077950778 @default.
- W2097377025 hasAuthorship W2097377025A5078409741 @default.
- W2097377025 hasAuthorship W2097377025A5091657418 @default.
- W2097377025 hasBestOaLocation W20973770251 @default.
- W2097377025 hasConcept C104317684 @default.
- W2097377025 hasConcept C123894998 @default.
- W2097377025 hasConcept C147483822 @default.
- W2097377025 hasConcept C153911025 @default.
- W2097377025 hasConcept C159047783 @default.
- W2097377025 hasConcept C159654299 @default.
- W2097377025 hasConcept C202878990 @default.
- W2097377025 hasConcept C203014093 @default.
- W2097377025 hasConcept C2522874641 @default.
- W2097377025 hasConcept C2778236600 @default.
- W2097377025 hasConcept C2779338263 @default.
- W2097377025 hasConcept C2781375147 @default.
- W2097377025 hasConcept C32611913 @default.
- W2097377025 hasConcept C40767141 @default.
- W2097377025 hasConcept C542903549 @default.
- W2097377025 hasConcept C55493867 @default.