Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097432956> ?p ?o ?g. }
- W2097432956 endingPage "1243" @default.
- W2097432956 startingPage "1236" @default.
- W2097432956 abstract "Abstract Protein microarrays or proteome chips are potentially powerful tools for comprehensive analysis of protein‐protein interactions. In interaction analysis, a set of immobilized proteins is arrayed on slides and each slide is probed with a set of fluorescently labeled proteins. Here we have developed and tested an in vitro protein microarray, in which both arraying and probing proteins were prepared by cell‐free translation. The in vitro synthesis of fluorescently labeled proteins was accomplished by a new method: a fluorophore‐puromycin conjugate was incorporated into a protein at the C ‐terminus on the ribosome. The resulting fluorescently labeled proteins were confirmed to be useful for probing protein‐protein interactions on protein microarrays in model experiments. Since the in vitro protein microarrays can easily be extended to a high‐throughput format and also combined with in vitro display technologies such as the streptavidin‐biotin linkage in emulsions method (Doi and Yanagawa, FEBS Lett . 1999, 457 , 227–230), our method should be useful for large‐scale analysis of protein‐protein interactions." @default.
- W2097432956 created "2016-06-24" @default.
- W2097432956 creator A5001376695 @default.
- W2097432956 creator A5012497365 @default.
- W2097432956 creator A5050563080 @default.
- W2097432956 creator A5052624691 @default.
- W2097432956 creator A5061901447 @default.
- W2097432956 creator A5082505038 @default.
- W2097432956 creator A5082693806 @default.
- W2097432956 creator A5086946782 @default.
- W2097432956 creator A5089093718 @default.
- W2097432956 date "2003-07-01" @default.
- W2097432956 modified "2023-10-16" @default.
- W2097432956 title "<b><i>In vitro</i></b> protein microarrays for detecting protein‐protein interactions: Application of a new method for fluorescence labeling of proteins" @default.
- W2097432956 cites W101393935 @default.
- W2097432956 cites W1526208457 @default.
- W2097432956 cites W1647597862 @default.
- W2097432956 cites W1669562987 @default.
- W2097432956 cites W1918211917 @default.
- W2097432956 cites W1970156673 @default.
- W2097432956 cites W1975085160 @default.
- W2097432956 cites W1976783038 @default.
- W2097432956 cites W1980211123 @default.
- W2097432956 cites W1981028296 @default.
- W2097432956 cites W1990557050 @default.
- W2097432956 cites W1990720034 @default.
- W2097432956 cites W1997197103 @default.
- W2097432956 cites W2004524502 @default.
- W2097432956 cites W2007698777 @default.
- W2097432956 cites W2010877045 @default.
- W2097432956 cites W2012383776 @default.
- W2097432956 cites W2028493269 @default.
- W2097432956 cites W2034999042 @default.
- W2097432956 cites W2036516702 @default.
- W2097432956 cites W2037036397 @default.
- W2097432956 cites W2040172043 @default.
- W2097432956 cites W2040575583 @default.
- W2097432956 cites W2042007309 @default.
- W2097432956 cites W2055760720 @default.
- W2097432956 cites W2057993402 @default.
- W2097432956 cites W2060145168 @default.
- W2097432956 cites W2060665625 @default.
- W2097432956 cites W2061455950 @default.
- W2097432956 cites W2065304353 @default.
- W2097432956 cites W2066631634 @default.
- W2097432956 cites W2070727822 @default.
- W2097432956 cites W2074064799 @default.
- W2097432956 cites W2077177643 @default.
- W2097432956 cites W2084619201 @default.
- W2097432956 cites W2090110765 @default.
- W2097432956 cites W2097463865 @default.
- W2097432956 cites W2106562836 @default.
- W2097432956 cites W2113654464 @default.
- W2097432956 cites W2121553505 @default.
- W2097432956 cites W2122476025 @default.
- W2097432956 cites W2138461857 @default.
- W2097432956 cites W2140393594 @default.
- W2097432956 cites W2145753039 @default.
- W2097432956 cites W2155445817 @default.
- W2097432956 cites W2163505253 @default.
- W2097432956 cites W2166558964 @default.
- W2097432956 cites W2329894300 @default.
- W2097432956 cites W4233751197 @default.
- W2097432956 cites W4252699563 @default.
- W2097432956 cites W4319053986 @default.
- W2097432956 doi "https://doi.org/10.1002/pmic.200300444" @default.
- W2097432956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12872224" @default.
- W2097432956 hasPublicationYear "2003" @default.
- W2097432956 type Work @default.
- W2097432956 sameAs 2097432956 @default.
- W2097432956 citedByCount "66" @default.
- W2097432956 countsByYear W20974329562012 @default.
- W2097432956 countsByYear W20974329562013 @default.
- W2097432956 countsByYear W20974329562014 @default.
- W2097432956 countsByYear W20974329562016 @default.
- W2097432956 countsByYear W20974329562018 @default.
- W2097432956 countsByYear W20974329562020 @default.
- W2097432956 countsByYear W20974329562023 @default.
- W2097432956 crossrefType "journal-article" @default.
- W2097432956 hasAuthorship W2097432956A5001376695 @default.
- W2097432956 hasAuthorship W2097432956A5012497365 @default.
- W2097432956 hasAuthorship W2097432956A5050563080 @default.
- W2097432956 hasAuthorship W2097432956A5052624691 @default.
- W2097432956 hasAuthorship W2097432956A5061901447 @default.
- W2097432956 hasAuthorship W2097432956A5082505038 @default.
- W2097432956 hasAuthorship W2097432956A5082693806 @default.
- W2097432956 hasAuthorship W2097432956A5086946782 @default.
- W2097432956 hasAuthorship W2097432956A5089093718 @default.
- W2097432956 hasConcept C104317684 @default.
- W2097432956 hasConcept C104397665 @default.
- W2097432956 hasConcept C121332964 @default.
- W2097432956 hasConcept C123894998 @default.
- W2097432956 hasConcept C140051345 @default.
- W2097432956 hasConcept C150194340 @default.
- W2097432956 hasConcept C153911025 @default.
- W2097432956 hasConcept C156904656 @default.
- W2097432956 hasConcept C185592680 @default.
- W2097432956 hasConcept C195814341 @default.