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- W2097478974 abstract "This study was conducted to induce experimental infection in rainbow trout with Y. ruckeri, the causative agent of ERM disease and progress of the disease was then monitored over the time period. Application of PCR for early and accurate diagnosis of infection was also investigated. Total 100 fish were allocated to four experimental and a control groupeach containing 20 fish. There were 20 aquaria and f ive fishwere housed in each aquaria. There were 4 replicates for each group.Fish,at anaverage weight of 75 g, were injected intramuscularly with 0.1 ml dilutions of Y. ruckeriat various doses (4x10 8 , 1x10 8 , 4x10 4 and 1x10 4 per ml) in the experimental groups. Blood samples were collected from fish that showed typical signs of the disease. DNAs extracted from blood samples were subjected to PCR amplification based upon a pair ofY. ruckerispecific primers. F ollowing agarose gel electrophoresis of the PCR prod ucts, positive bands with the molecular size of approximately 589 bp which were considered indicative for identification as Y. ruckeriwere observed. In addition, isolation and identification of Y. ruckerifrom various internal organs of infected fish were made by conventional culture method. The results suggested that early diagnosis of ERM disease can be made while fish are alive and diseased fish can be isolated and treated accordingly which will help decrease economic losses ." @default.
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- W2097478974 date "2011-01-01" @default.
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- W2097478974 title "Detection of Yersinia ruckeri by polymerase chain reaction (PCR) in infected rainbow trout (Oncorhynchus mykiss Walbaum 1792)." @default.
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