FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097484483> ?p ?o ?g. }

• W2097484483 endingPage "1511" @default.
• W2097484483 startingPage "1502" @default.
• W2097484483 abstract "ABSTRACT Herpesviruses have a characteristic particle structure comprising an icosahedral capsid, which contains the DNA genome and is, in turn, surrounded by a proteinaceous tegument layer and a lipid envelope. In herpes simplex virus, the interaction between the capsid and tegument is limited to the capsid vertices and involves two minor capsid proteins, pUL17 and pUL25, and the large inner tegument protein pUL36. pUL17 and pUL25 form a heterodimeric structure, the capsid vertex-specific component (CVSC), that lies on top of the peripentonal triplexes, while pUL36 has been reported to connect the CVSC to the penton. In this study, we used virus mutants with deletions in the genes for pUL36 and another inner tegument protein, pUL37, to analyze the contributions of these proteins to CVSC structure. Using electron cryomicroscopy and icosahedral reconstruction of mutants that express pUL17 and pUL25 but not pUL36, we showed that in contrast to accepted models, the CVSC is not formed from pUL17 and pUL25 on their own but requires a contribution from pUL36. In addition, the presence of full-length pUL36 results in weak density that extends the CVSC toward the penton, suggesting either that this extra density is formed directly by pUL36 or that pUL36 stabilizes other components of the vertex-tegument interface. IMPORTANCE Herpesviruses have complex particles that are formed as a result of a carefully controlled sequence of assembly steps. The nature of the interaction between two of the major particle compartments, the icosahedral capsid and the amorphous tegument, has been extensively studied, but the identity of the interacting proteins and their roles in forming the connections are still unclear. In this study, we used electron microscopy and three-dimensional reconstruction to analyze virus particles formed by mutants that do not express particular interacting proteins. We show that the largest viral protein, pUL36, which occupies the layer of tegument closest to the capsid, is essential for formation of structurally normal connections to the capsid. This demonstrates the importance of pUL36 in the initial stages of tegument addition and provides new insights into the process of virus particle assembly." @default.
• W2097484483 created "2016-06-24" @default.
• W2097484483 creator A5000794497 @default.
• W2097484483 creator A5001010613 @default.
• W2097484483 creator A5038820258 @default.
• W2097484483 creator A5049243674 @default.
• W2097484483 creator A5066546837 @default.
• W2097484483 creator A5076102972 @default.
• W2097484483 date "2015-02-01" @default.
• W2097484483 modified "2023-10-01" @default.
• W2097484483 title "The Large Tegument Protein pUL36 Is Essential for Formation of the Capsid Vertex-Specific Component at the Capsid-Tegument Interface of Herpes Simplex Virus 1" @default.
• W2097484483 cites W1515546984 @default.
• W2097484483 cites W1516969483 @default.
• W2097484483 cites W1541539119 @default.
• W2097484483 cites W1664735557 @default.
• W2097484483 cites W1731809180 @default.
• W2097484483 cites W1901313370 @default.
• W2097484483 cites W1968796953 @default.
• W2097484483 cites W1971456076 @default.
• W2097484483 cites W1972478587 @default.
• W2097484483 cites W1983855301 @default.
• W2097484483 cites W1985880387 @default.
• W2097484483 cites W1999985873 @default.
• W2097484483 cites W2002126032 @default.
• W2097484483 cites W2002256385 @default.
• W2097484483 cites W2003596982 @default.
• W2097484483 cites W2006158980 @default.
• W2097484483 cites W2006226787 @default.
• W2097484483 cites W2009043962 @default.
• W2097484483 cites W2014062503 @default.
• W2097484483 cites W2015628141 @default.
• W2097484483 cites W2021304970 @default.
• W2097484483 cites W2027929104 @default.
• W2097484483 cites W2032146255 @default.
• W2097484483 cites W2043776905 @default.
• W2097484483 cites W2048641058 @default.
• W2097484483 cites W2048982317 @default.
• W2097484483 cites W2051834975 @default.
• W2097484483 cites W2052319008 @default.
• W2097484483 cites W2053038074 @default.
• W2097484483 cites W2057431892 @default.
• W2097484483 cites W2061513932 @default.
• W2097484483 cites W2063033962 @default.
• W2097484483 cites W2076131096 @default.
• W2097484483 cites W2081460107 @default.
• W2097484483 cites W2086466935 @default.
• W2097484483 cites W2093542233 @default.
• W2097484483 cites W2100532542 @default.
• W2097484483 cites W2104130324 @default.
• W2097484483 cites W2106781770 @default.
• W2097484483 cites W2108518323 @default.
• W2097484483 cites W2108583777 @default.
• W2097484483 cites W2109073349 @default.
• W2097484483 cites W2117060330 @default.
• W2097484483 cites W2125968979 @default.
• W2097484483 cites W2132629607 @default.
• W2097484483 cites W2138395245 @default.
• W2097484483 cites W2141673999 @default.
• W2097484483 cites W2141698142 @default.
• W2097484483 cites W2144708209 @default.
• W2097484483 cites W2148111546 @default.
• W2097484483 cites W2150052212 @default.
• W2097484483 cites W2152460995 @default.
• W2097484483 cites W2153750826 @default.
• W2097484483 cites W2153830973 @default.
• W2097484483 cites W2154434172 @default.
• W2097484483 cites W2154780367 @default.
• W2097484483 cites W2158067882 @default.
• W2097484483 cites W2160571804 @default.
• W2097484483 cites W2161210875 @default.
• W2097484483 cites W2165155404 @default.
• W2097484483 cites W2165246104 @default.
• W2097484483 cites W2169222163 @default.
• W2097484483 cites W2170747141 @default.
• W2097484483 doi "https://doi.org/10.1128/jvi.02887-14" @default.
• W2097484483 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4300765" @default.
• W2097484483 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25410861" @default.
• W2097484483 hasPublicationYear "2015" @default.
• W2097484483 type Work @default.
• W2097484483 sameAs 2097484483 @default.
• W2097484483 citedByCount "44" @default.
• W2097484483 countsByYear W20974844832015 @default.
• W2097484483 countsByYear W20974844832016 @default.
• W2097484483 countsByYear W20974844832017 @default.
• W2097484483 countsByYear W20974844832018 @default.
• W2097484483 countsByYear W20974844832019 @default.
• W2097484483 countsByYear W20974844832020 @default.
• W2097484483 countsByYear W20974844832021 @default.
• W2097484483 countsByYear W20974844832022 @default.
• W2097484483 countsByYear W20974844832023 @default.
• W2097484483 crossrefType "journal-article" @default.
• W2097484483 hasAuthorship W2097484483A5000794497 @default.
• W2097484483 hasAuthorship W2097484483A5001010613 @default.
• W2097484483 hasAuthorship W2097484483A5038820258 @default.
• W2097484483 hasAuthorship W2097484483A5049243674 @default.
• W2097484483 hasAuthorship W2097484483A5066546837 @default.
• W2097484483 hasAuthorship W2097484483A5076102972 @default.
• W2097484483 hasBestOaLocation W20974844831 @default.

• next