Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097506848> ?p ?o ?g. }
- W2097506848 endingPage "1104" @default.
- W2097506848 startingPage "1093" @default.
- W2097506848 abstract "ABSTRACT The viral protein Nef contributes to the optimal infectivity of human and simian immunodeficiency viruses. The requirement for Nef during viral biogenesis particles suggests that Nef might play a role in this process. Alternatively, because Nef is incorporated into viruses, it might play a role when progeny virions reach target cells. We challenged these hypotheses by manipulating the amounts of Nef incorporated in viruses while keeping its expression level constant in producer cells. This was achieved by forcing the incorporation of Nef into viral particles by fusing a Vpr sequence to the C-terminal end of Nef. A cleavage site for the viral protease was introduced between Nef and Vpr to allow the release of Nef fragments from the fusion protein during virus maturation. We show that the resulting Nef-CS-Vpr fusion partially retains the ability of Nef to downregulate cell surface CD4 and that high amounts of Nef-CS-Vpr are incorporated into viral particles compared with what is seen for wild-type Nef. The fusion protein is processed during virion maturation and releases Nef fragments similar to those found in viruses produced in the presence of wild-type Nef. Unlike viruses produced in the presence of wild-type Nef, viruses produced in the presence of Nef-CS-Vpr do not have an increase in infectivity and are as poorly infectious as viruses produced in the absence of Nef. These findings demonstrate that the presence of Nef in viral particles is not sufficient to increase human immunodeficiency virus type 1 infectivity and suggest that Nef plays a role during the biogenesis of viral particles." @default.
- W2097506848 created "2016-06-24" @default.
- W2097506848 creator A5004396985 @default.
- W2097506848 creator A5020013376 @default.
- W2097506848 creator A5079297967 @default.
- W2097506848 date "2009-01-15" @default.
- W2097506848 modified "2023-10-12" @default.
- W2097506848 title "Human Immunodeficiency Virus Type 1 Nef Incorporation into Virions Does Not Increase Infectivity" @default.
- W2097506848 cites W1500244289 @default.
- W2097506848 cites W1528266646 @default.
- W2097506848 cites W1547922948 @default.
- W2097506848 cites W1606519969 @default.
- W2097506848 cites W1894956018 @default.
- W2097506848 cites W1905921278 @default.
- W2097506848 cites W1911218066 @default.
- W2097506848 cites W1917381511 @default.
- W2097506848 cites W1919588791 @default.
- W2097506848 cites W1965131361 @default.
- W2097506848 cites W1966288685 @default.
- W2097506848 cites W1966382788 @default.
- W2097506848 cites W1966478905 @default.
- W2097506848 cites W1966732827 @default.
- W2097506848 cites W1979542915 @default.
- W2097506848 cites W1981951679 @default.
- W2097506848 cites W1983597217 @default.
- W2097506848 cites W1986232807 @default.
- W2097506848 cites W1986684027 @default.
- W2097506848 cites W1989560607 @default.
- W2097506848 cites W1990719919 @default.
- W2097506848 cites W1991069890 @default.
- W2097506848 cites W1992311595 @default.
- W2097506848 cites W1997578300 @default.
- W2097506848 cites W2001243226 @default.
- W2097506848 cites W2001375348 @default.
- W2097506848 cites W2004640739 @default.
- W2097506848 cites W2007845379 @default.
- W2097506848 cites W2012690743 @default.
- W2097506848 cites W2014818809 @default.
- W2097506848 cites W2016266365 @default.
- W2097506848 cites W2022697293 @default.
- W2097506848 cites W2024694328 @default.
- W2097506848 cites W2026057161 @default.
- W2097506848 cites W2029379203 @default.
- W2097506848 cites W2034207391 @default.
- W2097506848 cites W2039300275 @default.
- W2097506848 cites W2040473151 @default.
- W2097506848 cites W2042471523 @default.
- W2097506848 cites W2044796731 @default.
- W2097506848 cites W2045106722 @default.
- W2097506848 cites W2045305626 @default.
- W2097506848 cites W2045519662 @default.
- W2097506848 cites W2045729285 @default.
- W2097506848 cites W2047951231 @default.
- W2097506848 cites W2054395379 @default.
- W2097506848 cites W2055209036 @default.
- W2097506848 cites W2057567758 @default.
- W2097506848 cites W2058436649 @default.
- W2097506848 cites W2064557078 @default.
- W2097506848 cites W2066062917 @default.
- W2097506848 cites W2071827167 @default.
- W2097506848 cites W2072901265 @default.
- W2097506848 cites W2073273867 @default.
- W2097506848 cites W2080774901 @default.
- W2097506848 cites W2082544893 @default.
- W2097506848 cites W2086960330 @default.
- W2097506848 cites W2092114045 @default.
- W2097506848 cites W2094504211 @default.
- W2097506848 cites W2095676386 @default.
- W2097506848 cites W2095679834 @default.
- W2097506848 cites W2096344281 @default.
- W2097506848 cites W2097074859 @default.
- W2097506848 cites W2097444764 @default.
- W2097506848 cites W2097799740 @default.
- W2097506848 cites W2104944781 @default.
- W2097506848 cites W2105630157 @default.
- W2097506848 cites W2107292352 @default.
- W2097506848 cites W2108912173 @default.
- W2097506848 cites W2110483459 @default.
- W2097506848 cites W2112156721 @default.
- W2097506848 cites W2113076274 @default.
- W2097506848 cites W2117098951 @default.
- W2097506848 cites W2118778549 @default.
- W2097506848 cites W2125393581 @default.
- W2097506848 cites W2127930176 @default.
- W2097506848 cites W2130285456 @default.
- W2097506848 cites W2132634936 @default.
- W2097506848 cites W2135153509 @default.
- W2097506848 cites W2139695852 @default.
- W2097506848 cites W2141972266 @default.
- W2097506848 cites W2143430925 @default.
- W2097506848 cites W2143661142 @default.
- W2097506848 cites W2146187278 @default.
- W2097506848 cites W2146847077 @default.
- W2097506848 cites W2148851107 @default.
- W2097506848 cites W2148960594 @default.
- W2097506848 cites W2150469985 @default.
- W2097506848 cites W2159844260 @default.
- W2097506848 cites W2160814797 @default.