Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097517504> ?p ?o ?g. }
- W2097517504 endingPage "8175" @default.
- W2097517504 startingPage "8167" @default.
- W2097517504 abstract "Conformational changes of proteins adsorbing on biomaterial surfaces affect biocompatibility. Titanium is among the most successful biomaterials; however, the mechanisms leading to its biocompatibility are not yet understood. The primary objective of this study was to investigate the conformation of human plasma fibrinogen (HPF) adsorbed on titanium oxide surfaces. A method for preparing ultraflat titanium substrates was developed. This allowed high-resolution investigation of both the titanium oxide surfaces and the adsorbed state of HPF. The titanium oxide surfaces were first imaged with an atomic force microscope in air and subsequently incubated in a solution of HPF in phosphate buffer and imaged in fluid with tapping mode AFM. The titanium oxide surfaces exhibited a root-mean-squared (RMS) roughness of (0.29 ± 0.03) nm over (1.00 × 1.00) μm2 areas. Different degrees of molecular order were found on the titanium oxide surface. In crystalline surface areas square lattices with parameters a0 = b0 ≈ 0.5 nm were resolved, which is consistent with the (001) planes of the titanium dioxide TiO2 rutile. Repetitive scanning of TiO2 surface areas resulted in the abrasion of the outermost TiO2 layers by the AFM tip to a depth varying between 0.3 and 1.0 nm. When imaging adsorbed HPF, individual molecules and aggregates, often joined through their D domains, were resolved, and the typical HPF multiglobular structure was observed. The mean length and height of single molecules were (46 ± 3) nm and (1.4 ± 0.2) nm, respectively. Additional features (chain segments) adjacent to the D and E domains were resolved and attributed to the α chains and their C-termini. The combination of AFM and the ultraflat titanium preparation method has proven successful for the high-resolution study of both the TiO2 surface and the adsorbed HPF." @default.
- W2097517504 created "2016-06-24" @default.
- W2097517504 creator A5034108292 @default.
- W2097517504 creator A5062053520 @default.
- W2097517504 creator A5077048347 @default.
- W2097517504 creator A5079588767 @default.
- W2097517504 date "2000-09-14" @default.
- W2097517504 modified "2023-09-27" @default.
- W2097517504 title "Human Plasma Fibrinogen Adsorption on Ultraflat Titanium Oxide Surfaces Studied with Atomic Force Microscopy" @default.
- W2097517504 cites W1907444416 @default.
- W2097517504 cites W1975667253 @default.
- W2097517504 cites W1977837166 @default.
- W2097517504 cites W1982820514 @default.
- W2097517504 cites W1993575880 @default.
- W2097517504 cites W2013854174 @default.
- W2097517504 cites W2014277425 @default.
- W2097517504 cites W2018691623 @default.
- W2097517504 cites W2019888372 @default.
- W2097517504 cites W2022212201 @default.
- W2097517504 cites W2025561491 @default.
- W2097517504 cites W2032913207 @default.
- W2097517504 cites W2033484281 @default.
- W2097517504 cites W2042053454 @default.
- W2097517504 cites W2043936219 @default.
- W2097517504 cites W2046404608 @default.
- W2097517504 cites W2048806783 @default.
- W2097517504 cites W2052113550 @default.
- W2097517504 cites W2058721525 @default.
- W2097517504 cites W2060088649 @default.
- W2097517504 cites W2064986267 @default.
- W2097517504 cites W2066834485 @default.
- W2097517504 cites W2076028335 @default.
- W2097517504 cites W2077019855 @default.
- W2097517504 cites W2085437011 @default.
- W2097517504 cites W2147579383 @default.
- W2097517504 cites W2149482213 @default.
- W2097517504 cites W2160124075 @default.
- W2097517504 cites W2170678696 @default.
- W2097517504 cites W2394708838 @default.
- W2097517504 cites W3150248096 @default.
- W2097517504 cites W4248691724 @default.
- W2097517504 cites W4324262994 @default.
- W2097517504 doi "https://doi.org/10.1021/la000362k" @default.
- W2097517504 hasPublicationYear "2000" @default.
- W2097517504 type Work @default.
- W2097517504 sameAs 2097517504 @default.
- W2097517504 citedByCount "164" @default.
- W2097517504 countsByYear W20975175042012 @default.
- W2097517504 countsByYear W20975175042013 @default.
- W2097517504 countsByYear W20975175042014 @default.
- W2097517504 countsByYear W20975175042015 @default.
- W2097517504 countsByYear W20975175042016 @default.
- W2097517504 countsByYear W20975175042017 @default.
- W2097517504 countsByYear W20975175042018 @default.
- W2097517504 countsByYear W20975175042019 @default.
- W2097517504 countsByYear W20975175042020 @default.
- W2097517504 countsByYear W20975175042022 @default.
- W2097517504 countsByYear W20975175042023 @default.
- W2097517504 crossrefType "journal-article" @default.
- W2097517504 hasAuthorship W2097517504A5034108292 @default.
- W2097517504 hasAuthorship W2097517504A5062053520 @default.
- W2097517504 hasAuthorship W2097517504A5077048347 @default.
- W2097517504 hasAuthorship W2097517504A5079588767 @default.
- W2097517504 hasConcept C113196181 @default.
- W2097517504 hasConcept C127413603 @default.
- W2097517504 hasConcept C147789679 @default.
- W2097517504 hasConcept C150394285 @default.
- W2097517504 hasConcept C159985019 @default.
- W2097517504 hasConcept C166524527 @default.
- W2097517504 hasConcept C171250308 @default.
- W2097517504 hasConcept C178790620 @default.
- W2097517504 hasConcept C185592680 @default.
- W2097517504 hasConcept C191897082 @default.
- W2097517504 hasConcept C192562407 @default.
- W2097517504 hasConcept C2777230088 @default.
- W2097517504 hasConcept C2777593239 @default.
- W2097517504 hasConcept C2777889555 @default.
- W2097517504 hasConcept C2778414984 @default.
- W2097517504 hasConcept C2779851234 @default.
- W2097517504 hasConcept C32909587 @default.
- W2097517504 hasConcept C42360764 @default.
- W2097517504 hasConcept C43617362 @default.
- W2097517504 hasConcept C506065880 @default.
- W2097517504 hasConceptScore W2097517504C113196181 @default.
- W2097517504 hasConceptScore W2097517504C127413603 @default.
- W2097517504 hasConceptScore W2097517504C147789679 @default.
- W2097517504 hasConceptScore W2097517504C150394285 @default.
- W2097517504 hasConceptScore W2097517504C159985019 @default.
- W2097517504 hasConceptScore W2097517504C166524527 @default.
- W2097517504 hasConceptScore W2097517504C171250308 @default.
- W2097517504 hasConceptScore W2097517504C178790620 @default.
- W2097517504 hasConceptScore W2097517504C185592680 @default.
- W2097517504 hasConceptScore W2097517504C191897082 @default.
- W2097517504 hasConceptScore W2097517504C192562407 @default.
- W2097517504 hasConceptScore W2097517504C2777230088 @default.
- W2097517504 hasConceptScore W2097517504C2777593239 @default.
- W2097517504 hasConceptScore W2097517504C2777889555 @default.
- W2097517504 hasConceptScore W2097517504C2778414984 @default.