Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097524921> ?p ?o ?g. }
- W2097524921 endingPage "421" @default.
- W2097524921 startingPage "411" @default.
- W2097524921 abstract "The human hyperpolarization-activated cyclic nucleotide-gated 1 (hHCN1) subunit was heterologously expressed in mammalian cell lines (CV-1 and CHO) and its properties investigated using whole-cell patch-clamp recordings. Activation of this recombinant channel, by membrane hyperpolarization, generated a slowly activating, noninactivating inward current. The pharmacological properties of hHCN1-mediated currents resembled those of native hyperpolarization-activated currents (I(h)), that is, blockade by Cs(+) (99% at 5 mm), ZD 7288 (98% at 100 microm) and zatebradine (92% at 10 microm). Inhibition of the hHCN1-mediated current by ZD 7288 was apparently independent of prior channel activation (i.e. non-use-dependent), whereas that induced by zatebradine was use-dependent. The VR1 receptor antagonist capsazepine inhibited hHCN1-mediated currents in a concentration-dependent (IC(50)=8 microm), reversible and apparently non-use-dependent manner. This inhibitory effect of capsazepine was voltage-independent and associated with a leftward shift in the hHCN1 activation curve as well as a dramatic slowing of the kinetics of current activation. Elevation of intracellular cAMP or extracellular K(+) significantly enhanced aspects of hHCN1 currents. However, these manipulations did not significantly affect the capsazepine-induced inhibition of hHCN1. The development of structural analogues of capsazepine may yield compounds that could selectively inhibit HCN channels and prove useful for the treatment of neurological disorders where a role for HCN channels has been described." @default.
- W2097524921 created "2016-06-24" @default.
- W2097524921 creator A5015824905 @default.
- W2097524921 creator A5018828484 @default.
- W2097524921 creator A5019383039 @default.
- W2097524921 creator A5023756649 @default.
- W2097524921 creator A5023865728 @default.
- W2097524921 creator A5031775664 @default.
- W2097524921 creator A5047677418 @default.
- W2097524921 creator A5062531780 @default.
- W2097524921 creator A5074232553 @default.
- W2097524921 creator A5075924009 @default.
- W2097524921 creator A5081300350 @default.
- W2097524921 creator A5086807788 @default.
- W2097524921 creator A5088642953 @default.
- W2097524921 date "2004-10-01" @default.
- W2097524921 modified "2023-10-15" @default.
- W2097524921 title "Characterization of the human HCN1 channel and its inhibition by capsazepine" @default.
- W2097524921 cites W1800327488 @default.
- W2097524921 cites W1963531856 @default.
- W2097524921 cites W1965000550 @default.
- W2097524921 cites W1974273055 @default.
- W2097524921 cites W1974549206 @default.
- W2097524921 cites W1978734924 @default.
- W2097524921 cites W1981835164 @default.
- W2097524921 cites W1983678541 @default.
- W2097524921 cites W1987873309 @default.
- W2097524921 cites W1988080361 @default.
- W2097524921 cites W1998698456 @default.
- W2097524921 cites W2004855428 @default.
- W2097524921 cites W2006132476 @default.
- W2097524921 cites W2010973571 @default.
- W2097524921 cites W2011272321 @default.
- W2097524921 cites W2012064273 @default.
- W2097524921 cites W2015268846 @default.
- W2097524921 cites W2016334402 @default.
- W2097524921 cites W2018527368 @default.
- W2097524921 cites W2020505352 @default.
- W2097524921 cites W2023486326 @default.
- W2097524921 cites W2039347902 @default.
- W2097524921 cites W2063286250 @default.
- W2097524921 cites W2066559312 @default.
- W2097524921 cites W2070031472 @default.
- W2097524921 cites W2090736494 @default.
- W2097524921 cites W2102160264 @default.
- W2097524921 cites W2106201634 @default.
- W2097524921 cites W2112196515 @default.
- W2097524921 cites W2118621245 @default.
- W2097524921 cites W2132785335 @default.
- W2097524921 cites W2140845062 @default.
- W2097524921 cites W2165340969 @default.
- W2097524921 cites W2171502806 @default.
- W2097524921 cites W2395879567 @default.
- W2097524921 cites W2403995597 @default.
- W2097524921 cites W2471685616 @default.
- W2097524921 cites W4241209760 @default.
- W2097524921 doi "https://doi.org/10.1038/sj.bjp.0705945" @default.
- W2097524921 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1575350" @default.
- W2097524921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15351778" @default.
- W2097524921 hasPublicationYear "2004" @default.
- W2097524921 type Work @default.
- W2097524921 sameAs 2097524921 @default.
- W2097524921 citedByCount "49" @default.
- W2097524921 countsByYear W20975249212012 @default.
- W2097524921 countsByYear W20975249212013 @default.
- W2097524921 countsByYear W20975249212014 @default.
- W2097524921 countsByYear W20975249212015 @default.
- W2097524921 countsByYear W20975249212016 @default.
- W2097524921 countsByYear W20975249212017 @default.
- W2097524921 countsByYear W20975249212018 @default.
- W2097524921 countsByYear W20975249212019 @default.
- W2097524921 countsByYear W20975249212020 @default.
- W2097524921 countsByYear W20975249212021 @default.
- W2097524921 countsByYear W20975249212022 @default.
- W2097524921 countsByYear W20975249212023 @default.
- W2097524921 crossrefType "journal-article" @default.
- W2097524921 hasAuthorship W2097524921A5015824905 @default.
- W2097524921 hasAuthorship W2097524921A5018828484 @default.
- W2097524921 hasAuthorship W2097524921A5019383039 @default.
- W2097524921 hasAuthorship W2097524921A5023756649 @default.
- W2097524921 hasAuthorship W2097524921A5023865728 @default.
- W2097524921 hasAuthorship W2097524921A5031775664 @default.
- W2097524921 hasAuthorship W2097524921A5047677418 @default.
- W2097524921 hasAuthorship W2097524921A5062531780 @default.
- W2097524921 hasAuthorship W2097524921A5074232553 @default.
- W2097524921 hasAuthorship W2097524921A5075924009 @default.
- W2097524921 hasAuthorship W2097524921A5081300350 @default.
- W2097524921 hasAuthorship W2097524921A5086807788 @default.
- W2097524921 hasAuthorship W2097524921A5088642953 @default.
- W2097524921 hasBestOaLocation W20975249211 @default.
- W2097524921 hasConcept C104317684 @default.
- W2097524921 hasConcept C12554922 @default.
- W2097524921 hasConcept C131453863 @default.
- W2097524921 hasConcept C131829789 @default.
- W2097524921 hasConcept C147944092 @default.
- W2097524921 hasConcept C155164980 @default.
- W2097524921 hasConcept C160145004 @default.
- W2097524921 hasConcept C170493617 @default.