FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097599449> ?p ?o ?g. }

• W2097599449 endingPage "2646" @default.
• W2097599449 startingPage "2636" @default.
• W2097599449 abstract "Pyridoxal 5‘-phosphate (PLP) binds tightly to bovine low Mr protein tyrosine phosphatase (BPTP), but it is a very poor substrate for the enzyme. The structural basis of this tight binding of PLP is examined here by a variety of methods. Binding constants of a number of PLP analogues were measured with wild-type BPTP, and PLP binding constants of some site-specific mutants of BPTP were determined at pH 5.0 through the use of several independent methods. The tight binding of PLP (Ki = 7.6 μM) causes a downfield shift of the His-72 Cε1H resonance in the 1H NMR spectrum of the protein, consistent with a structural alteration in the phosphate binding loop transmitted through a complex hydrogen bond network that exists between His-72 and Asn-15, which is a residue in the phosphate binding loop. 1H NMR spectroscopy with an MLEV-17 spectral editing scheme was used to monitor the aldehyde resonance of PLP during titration of a catalytically inactive C12A mutant of BPTP. The aldehydic proton resonance of PLP shifted from 10.43 to 10.26 ppm upon complex formation with the C12A mutant. This resonance occurs far from the region where a hemithioacetal hydrogen would be expected to appear, consistent with the conclusion that the Cys-17 side chain of BPTP does not add to the aldehyde group of PLP. UV−visible spectrophotometric titration also supported this conclusion. The binding constant of PLP to a C17A mutant was similar to that exhibited with wild-type protein. These results show that Cys-17 makes virtually no contribution to the tight binding of PLP by BPTP, in contrast to a published report that it is “essential” for binding PLP. On the other hand, Asp-129 of BPTP was found to be very important for binding PLP. It is concluded that Asp-129 binds to the pyridinium nitrogen of PLP and that this renders Asp-129 effectively unavailable to serve its essential catalytic role as a general acid. The interactions described here should be useful in the design of specific inhibitors of this and related phosphotyrosyl protein phosphatases." @default.
• W2097599449 created "2016-06-24" @default.
• W2097599449 creator A5054017835 @default.
• W2097599449 creator A5087286917 @default.
• W2097599449 date "1999-02-10" @default.
• W2097599449 modified "2023-09-24" @default.
• W2097599449 title "Structural Basis of the Tight Binding of Pyridoxal 5‘-Phosphate to a Low Molecular Weight Protein Tyrosine Phosphatase^†" @default.
• W2097599449 cites W1661428152 @default.
• W2097599449 cites W1693419395 @default.
• W2097599449 cites W2051899270 @default.
• W2097599449 cites W2069528909 @default.
• W2097599449 cites W2086195728 @default.
• W2097599449 cites W2097558464 @default.
• W2097599449 cites W2330138679 @default.
• W2097599449 cites W253339776 @default.
• W2097599449 cites W3004565440 @default.
• W2097599449 doi "https://doi.org/10.1021/bi9823737" @default.
• W2097599449 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10052933" @default.
• W2097599449 hasPublicationYear "1999" @default.
• W2097599449 type Work @default.
• W2097599449 sameAs 2097599449 @default.
• W2097599449 citedByCount "12" @default.
• W2097599449 countsByYear W20975994492012 @default.
• W2097599449 countsByYear W20975994492015 @default.
• W2097599449 countsByYear W20975994492020 @default.
• W2097599449 crossrefType "journal-article" @default.
• W2097599449 hasAuthorship W2097599449A5054017835 @default.
• W2097599449 hasAuthorship W2097599449A5087286917 @default.
• W2097599449 hasConcept C107824862 @default.
• W2097599449 hasConcept C112887158 @default.
• W2097599449 hasConcept C178790620 @default.
• W2097599449 hasConcept C181199279 @default.
• W2097599449 hasConcept C184866935 @default.
• W2097599449 hasConcept C185592680 @default.
• W2097599449 hasConcept C197957613 @default.
• W2097599449 hasConcept C2776165026 @default.
• W2097599449 hasConcept C2776834122 @default.
• W2097599449 hasConcept C2780859938 @default.
• W2097599449 hasConcept C32909587 @default.
• W2097599449 hasConcept C41183919 @default.
• W2097599449 hasConcept C55493867 @default.
• W2097599449 hasConcept C66974803 @default.
• W2097599449 hasConcept C71240020 @default.
• W2097599449 hasConcept C85528070 @default.
• W2097599449 hasConceptScore W2097599449C107824862 @default.
• W2097599449 hasConceptScore W2097599449C112887158 @default.
• W2097599449 hasConceptScore W2097599449C178790620 @default.
• W2097599449 hasConceptScore W2097599449C181199279 @default.
• W2097599449 hasConceptScore W2097599449C184866935 @default.
• W2097599449 hasConceptScore W2097599449C185592680 @default.
• W2097599449 hasConceptScore W2097599449C197957613 @default.
• W2097599449 hasConceptScore W2097599449C2776165026 @default.
• W2097599449 hasConceptScore W2097599449C2776834122 @default.
• W2097599449 hasConceptScore W2097599449C2780859938 @default.
• W2097599449 hasConceptScore W2097599449C32909587 @default.
• W2097599449 hasConceptScore W2097599449C41183919 @default.
• W2097599449 hasConceptScore W2097599449C55493867 @default.
• W2097599449 hasConceptScore W2097599449C66974803 @default.
• W2097599449 hasConceptScore W2097599449C71240020 @default.
• W2097599449 hasConceptScore W2097599449C85528070 @default.
• W2097599449 hasIssue "9" @default.
• W2097599449 hasLocation W20975994491 @default.
• W2097599449 hasLocation W20975994492 @default.
• W2097599449 hasOpenAccess W2097599449 @default.
• W2097599449 hasPrimaryLocation W20975994491 @default.
• W2097599449 hasRelatedWork W2018469585 @default.
• W2097599449 hasRelatedWork W2025931729 @default.
• W2097599449 hasRelatedWork W2048372851 @default.
• W2097599449 hasRelatedWork W2051757307 @default.
• W2097599449 hasRelatedWork W2091286894 @default.
• W2097599449 hasRelatedWork W2091472909 @default.
• W2097599449 hasRelatedWork W2095003093 @default.
• W2097599449 hasRelatedWork W2163088472 @default.
• W2097599449 hasRelatedWork W2469739895 @default.
• W2097599449 hasRelatedWork W2808318619 @default.
• W2097599449 hasVolume "38" @default.
• W2097599449 isParatext "false" @default.
• W2097599449 isRetracted "false" @default.
• W2097599449 magId "2097599449" @default.
• W2097599449 workType "article" @default.