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- W2097613634 abstract "Efficient target gene delivery into eukaryotic cells is important for biotechnological research and gene therapy. Gene delivery based on proteins, including histones, has recently emerged as a powerful non-viral DNA transfer technique. Here, we investigated the potential use of a recombinant mussel adhesive protein, hybrid fp-151, as a gene delivery material, in view of its similar basic amino acid composition to histone proteins, and cost-effective and high-level production in Escherichia coli. After confirming DNA binding affinity, we transfected mammalian cells (human 293T and mouse NIH/3T3) with foreign genes using hybrid fp-151 as the gene delivery carrier. Hybrid fp-151 displayed comparable transfection efficiency in both mammalian cell lines, compared to the widely used transfection agent, Lipofectamine™ 2000. Our results indicate that this mussel adhesive protein may be used as a potential protein-based gene-transfer mediator. Biotechnol. Bioeng. 2009;102: 616–623. © 2008 Wiley Periodicals, Inc." @default.
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- W2097613634 date "2009-02-01" @default.
- W2097613634 modified "2023-10-03" @default.
- W2097613634 title "Recombinant mussel adhesive protein as a gene delivery material" @default.
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- W2097613634 doi "https://doi.org/10.1002/bit.22086" @default.
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