FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2097624027> ?p ?o ?g. }

• W2097624027 endingPage "S54" @default.
• W2097624027 startingPage "S54" @default.
• W2097624027 abstract "OBJECTIVE: The aim of this study was to determine whether exposure to Activin-A followed by FSH would enhance growth and development of isolated human preantral follicles within a recently developed two-step serum free culture system.DESIGN: Ovarian cortical biopsies of approximately 5x4mm were obtained after informed consent during laparoscopic oophorectomy (n=2) and at caesarean section (n=1) from woman aged 40-41 years. Approval for this study was given by the local ethics committee.MATERIALS AND METHODS: Ovarian cortical strips containing primordial and primary follicles were cultured in serum free medium (McCoys + human serum albumen) for 6 days. At the end of this culture period secondary follicles were isolated from the strips. Intact secondary follicles with a mean diameter of 74 +/−5 microns (n = 33) were selected for further culture. Isolated follicles were placed individually within v shaped micro well culture plates in serum free medium in the presence of 100ng/ml of human recombinant activin A. After 2 days 50ng/ml of rhFSH in combination with 100ng/ml of activin A was added to approximately half of the cultured follicles (n=18) whilst the remainder (n=15) were exposed to only activin A for a further 2 days. Follicle diameters were measured on day 2 and day 4, cultured follicles at day 4 were fixed in Bouin's solution and prepared for histology and subsequent morphological analysis to determine oocyte and granulosa cell health.RESULTS: Isolated preantral follicles showed a significant increase in size during the first 2 days of culture in the presence of activin A. No follicular rupture or degeneration was observed during this period. Follicles exposed to FSH after 2 days in vitro showed a significantly higher increase in size (130+/−22 microns) when compared with those cultured in activin alone (91 +/− 13 microns) (p< 0.05). Morphological analysis showed an improvement in follicle health in those follicles exposed to both activin A and FSH.CONCLUSIONS: Primordial follicles can be activated to grow in vitro and develop to preantral follicles within 6 days of culture in cortical strips. Isolated preantral follicles show significant growth during a further 4 day culture period in the presence of activin A. Follicles exposed to a combination of activin A and FSH show a significantly greater rate of growth and improved morphology compared to activin alone. These preliminary results represent further progress towards achieving full in vitro growth of human oocytes. OBJECTIVE: The aim of this study was to determine whether exposure to Activin-A followed by FSH would enhance growth and development of isolated human preantral follicles within a recently developed two-step serum free culture system. DESIGN: Ovarian cortical biopsies of approximately 5x4mm were obtained after informed consent during laparoscopic oophorectomy (n=2) and at caesarean section (n=1) from woman aged 40-41 years. Approval for this study was given by the local ethics committee. MATERIALS AND METHODS: Ovarian cortical strips containing primordial and primary follicles were cultured in serum free medium (McCoys + human serum albumen) for 6 days. At the end of this culture period secondary follicles were isolated from the strips. Intact secondary follicles with a mean diameter of 74 +/−5 microns (n = 33) were selected for further culture. Isolated follicles were placed individually within v shaped micro well culture plates in serum free medium in the presence of 100ng/ml of human recombinant activin A. After 2 days 50ng/ml of rhFSH in combination with 100ng/ml of activin A was added to approximately half of the cultured follicles (n=18) whilst the remainder (n=15) were exposed to only activin A for a further 2 days. Follicle diameters were measured on day 2 and day 4, cultured follicles at day 4 were fixed in Bouin's solution and prepared for histology and subsequent morphological analysis to determine oocyte and granulosa cell health. RESULTS: Isolated preantral follicles showed a significant increase in size during the first 2 days of culture in the presence of activin A. No follicular rupture or degeneration was observed during this period. Follicles exposed to FSH after 2 days in vitro showed a significantly higher increase in size (130+/−22 microns) when compared with those cultured in activin alone (91 +/− 13 microns) (p< 0.05). Morphological analysis showed an improvement in follicle health in those follicles exposed to both activin A and FSH. CONCLUSIONS: Primordial follicles can be activated to grow in vitro and develop to preantral follicles within 6 days of culture in cortical strips. Isolated preantral follicles show significant growth during a further 4 day culture period in the presence of activin A. Follicles exposed to a combination of activin A and FSH show a significantly greater rate of growth and improved morphology compared to activin alone. These preliminary results represent further progress towards achieving full in vitro growth of human oocytes." @default.
• W2097624027 created "2016-06-24" @default.
• W2097624027 creator A5019395613 @default.
• W2097624027 creator A5022777994 @default.
• W2097624027 creator A5040050696 @default.
• W2097624027 creator A5042263357 @default.
• W2097624027 creator A5045226918 @default.
• W2097624027 date "2008-09-01" @default.
• W2097624027 modified "2023-09-30" @default.
• W2097624027 title "Development of human preantral follicles in vitro is enhanced by timed exposure to activin-A followed by FSH" @default.
• W2097624027 doi "https://doi.org/10.1016/j.fertnstert.2008.07.726" @default.
• W2097624027 hasPublicationYear "2008" @default.
• W2097624027 type Work @default.
• W2097624027 sameAs 2097624027 @default.
• W2097624027 citedByCount "2" @default.
• W2097624027 crossrefType "journal-article" @default.
• W2097624027 hasAuthorship W2097624027A5019395613 @default.
• W2097624027 hasAuthorship W2097624027A5022777994 @default.
• W2097624027 hasAuthorship W2097624027A5040050696 @default.
• W2097624027 hasAuthorship W2097624027A5042263357 @default.
• W2097624027 hasAuthorship W2097624027A5045226918 @default.
• W2097624027 hasBestOaLocation W20976240271 @default.
• W2097624027 hasConcept C102570824 @default.
• W2097624027 hasConcept C126322002 @default.
• W2097624027 hasConcept C134018914 @default.
• W2097624027 hasConcept C16685009 @default.
• W2097624027 hasConcept C196843134 @default.
• W2097624027 hasConcept C2776690073 @default.
• W2097624027 hasConcept C2778324911 @default.
• W2097624027 hasConcept C2780536345 @default.
• W2097624027 hasConcept C5512455 @default.
• W2097624027 hasConcept C71924100 @default.
• W2097624027 hasConcept C86803240 @default.
• W2097624027 hasConcept C87073359 @default.
• W2097624027 hasConcept C95444343 @default.
• W2097624027 hasConceptScore W2097624027C102570824 @default.
• W2097624027 hasConceptScore W2097624027C126322002 @default.
• W2097624027 hasConceptScore W2097624027C134018914 @default.
• W2097624027 hasConceptScore W2097624027C16685009 @default.
• W2097624027 hasConceptScore W2097624027C196843134 @default.
• W2097624027 hasConceptScore W2097624027C2776690073 @default.
• W2097624027 hasConceptScore W2097624027C2778324911 @default.
• W2097624027 hasConceptScore W2097624027C2780536345 @default.
• W2097624027 hasConceptScore W2097624027C5512455 @default.
• W2097624027 hasConceptScore W2097624027C71924100 @default.
• W2097624027 hasConceptScore W2097624027C86803240 @default.
• W2097624027 hasConceptScore W2097624027C87073359 @default.
• W2097624027 hasConceptScore W2097624027C95444343 @default.
• W2097624027 hasLocation W20976240271 @default.
• W2097624027 hasOpenAccess W2097624027 @default.
• W2097624027 hasPrimaryLocation W20976240271 @default.
• W2097624027 hasRelatedWork W1982656190 @default.
• W2097624027 hasRelatedWork W2045822318 @default.
• W2097624027 hasRelatedWork W2068375641 @default.
• W2097624027 hasRelatedWork W2081394320 @default.
• W2097624027 hasRelatedWork W2114542496 @default.
• W2097624027 hasRelatedWork W2148951415 @default.
• W2097624027 hasRelatedWork W2164328274 @default.
• W2097624027 hasRelatedWork W2172100709 @default.
• W2097624027 hasRelatedWork W3151884350 @default.
• W2097624027 hasRelatedWork W178837743 @default.
• W2097624027 hasVolume "90" @default.
• W2097624027 isParatext "false" @default.
• W2097624027 isRetracted "false" @default.
• W2097624027 magId "2097624027" @default.
• W2097624027 workType "article" @default.